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1996 Fiscal Year Final Research Report Summary

Biochemical and Molecular Biological S tudies on Isoprenoid

Research Project

Project/Area Number 08044052
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

OGURA Kyozo  Tohoku, Univ., Institute for Chemical Reaction Science professor, 反応化学研究所, 教授 (80006303)

Co-Investigator(Kenkyū-buntansha) LENNARZ W.J  State Univ.of New York, Department of Biochemistry and Cell Biology Professor, 生化学及び細胞生物化学科, 教授
SHIDOGI Yoshihiro  Gifu Univ.School of Medicine, First Department of Internal Medicine Research Ass, 医学部, 助手 (00111518)
TANAKA Yasuyuki  Tokyo Univ.of Agricultuer and Technology, Faculty of Technology professor, 工学部, 教授 (80015114)
KOYAMA Tanetoshi  Tohoku, Univ., Department of Biochemistry and Engineering Associate Professor, 助教授 (20089808)
SAGAMI Hiroshi  Tohoku, Univ., Institute for Chemical Reaction Science Associate Professor, 反応化学研究所, 助教授 (10134058)
Project Period (FY) 1996
Keywordsprenyltransferase / affinity labeling / isoprenoid / geranylgeranoicacid / dolichol / polyprenol / natural rubber
Research Abstract

In order to promote and stimulate the mechanistic studies of enzymes involved in the biosynthesis of physiologically important isoprenoids, we invited five eminent American researchers working actively in this field to exchange information with them. Based on the result of discussion, three pieces of joint-research have been started as follows :
1) Studies on the active center of prenyltransferase by means of photoaffinity labeling Collaborator : G.D.Prestwich (Utah University)
2) Studies on the geranylgeranoate receptor Collabortor : Y.Shidoji (Gifu University)
3) Studies on enzymens involved in the biosynthesis of natural rubber Collaborator : Y.Tanaka (Tokyo University of Agriculture and Technology)

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] H.Sagami et al.: "Enzymatic Formation of Dehydrodolichal and Dolichal,New Products Related to Yeast Dolichol Biosynthesis" J.Biol.Chem.271. 9560-9566 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Koyama et al.: "Identification of Significant Residues in the Substrate Binding Site of Farnesyl Diphosphate Synthase" Biochemistry. 35. 9533-9538 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Ohnuma et al.: "Conversion from Farnesyl Diphosphate Synthase to Geranylgeranyl Diphosphate Synthase by Random Chemical Mutagenesis" J.Biol.Chem.271. 10087-10095 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Kurisaki et al.: "Proteolytic Release of Dehydrodolichyl Diphosphate Synthase from Pig Testis Microsome" Biosci.Biotech.Biochem.60. 1109-1114 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Koyama et al.: "Isopentenyl Diphosphate Isomerase in Rubber Latex" Phytochemistry. 43. 769-772 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Kurisaki et al.: "Distribution of Polyprenols and Dolichols in Soybean Plant" Phytochemistry. 44. 45-50 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Koike-Takeshita et al.: "J.Biol.Chem." Identification of Novel Gene Cluster Participating in Menaquinone (Vitamin K_2) Biosynthesis. Cloning and Sequence Determination of the 2-Heptaprenyl-1,4-naphthoquinone Methyltransferase Gene of Bacillus stearothermophilus, ((in press))

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Zhan et al.: "J.Bacteriol." Two Cistrons of the gerC Operon of Bacillus subtilis Encode the Two Subunits Heptaprenyl Diphosphate Synthase, ((in press))

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Ohkura et al.: "J.Biol.Chem." A Partial Defeciency of Dehydrodolichol Reduction is a Cause of Carbohydrate-Defecient Glycoprotein Syndrome TypeI, ((in press))

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Ogura: "Polyprenyl Diphosphate Synthases" Plenum Publishing Corporation (in press),

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Ogura,T.Koyama: "Mechanistic Enzymology and Molecular Cenetics of Chain Elongation in Isoprenoid Biosynthesis" Kodansha Scientific, 23 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Sagami et al: "Enzymatic Formation of Dehydrodolichal and Dolichal, New Products Related to Yeast Dolichol Biosynthesis" J.Biol.Chem. 271. 9560-9566 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Koyama et al: "Identification of Significant Residues in the Subustrate Binding Site of Farnesyl Diphosphate Synthase" Biochemistry. 35. 9533-9538 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Ohnuma et al: "Conversion from Farnesy Diphoshoate Synthase to Geranylgeranyl Diphoshate Synthase by Random Chemical Mutagenesis" J.Biol.Chem. 271. 10087-10095 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Kurisaki et al: "Proteolytic Release of Dehydrodolichyl Diphosphate Synthase from Pig Testis Microsomes" Biosci.Biotech.Biochem. 60. 1109-1114 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Koyama et al: "Isopentenyl Diphoshate Isomerase in Rubber Latex" Phytochemistry. 43. 769-772 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Kurisaki et al: "Distribution of Polyprenols and Dolichols in Soybean Plant" Phytochemistry. 44. 45-50 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Koike-Takeshita et al: "Identification of Novel Gene Cluster Participating in Menaquinone (Vitamin K_2) Biosynthesis. Cloning and Sequence Determination of the 2-Heptaprenyl-1,4-naphthoquinone Methyltransferase Gene of Bacillus stearothermophilus" J.Biol.Chem. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Zhang et al: "Two Cistrons of the gerc Operon of Bacillus subtilis Encode the Two Subunits Heptaprenyl Diphosphate Synthase" J.Bacteriol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Ohkura et al: "A Partial Defeciency of Dehydrodolichol Reduction is a Cause od Carbohydrate-Defecient Glycoprotein Syndrome Type I" J.Biol.Chem. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Ogura et al: Mechanistic Enzymology and Molecular Genetics of Chain Elongation in Isoprenoid Biosynthesis. "Dynamic Aspects of Natural Products Chemintry" , Kodansha Scientific, Tokyo, K.Ohura and U.Sankawa ed., 1-23 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Ogura et al: Polyprenyl Diphoshate Synthases. "Subcellular Biochemistry, Vol.28, Cholesterol : Its Functions and Metabolism in Biology and Medicine" , Plenum Publishing Corporation, London, J.R.Harris ed., in press,

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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