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1998 Fiscal Year Final Research Report Summary

Development of New Glycosyltransferase Inhibitors

Research Project

Project/Area Number 08044129
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field 高分子合成
Research InstitutionTokyo Institute of Technology

Principal Investigator

HATANAKA Kenichi  Tokyo Institute of Technology, Faculty of Bioscience and Biotechnology, Associate Professor, 生命理工学部, 助教授 (70167584)

Co-Investigator(Kenkyū-buntansha) ヤンツェン パン  アーカンソー大学, 生化学分子生物学科, 助教授
アラン エルバイン  アーカンソー大学, 生化学分子生物学科, 主任教授
KOBAYASHI Yuichi  Tokyo Institute of Technology, Faculty of Bioscience and Biotechnology, Associat, 生命理工学部, 助教授 (90153650)
PAN Yuan Tseng  University of Arkansas, Department of Biochemistry and Molecular Biology, Resear
ELBEIN Alan D.  University of Arkansas, Department of Biochemistry and Molecular Biology, Chairm
Project Period (FY) 1996 – 1998
KeywordsCridine / Glycosyl Transferase / Inhibitor / Cell Adhesion / Polystyrene / Biopolymer
Research Abstract

2' , 3' -Di-O-acetyluridine 5' -p-styrenesulfonate was synthesized by the reaction of 2' , 3' -di-O-acetyluridine with p-styrenesulfonyl chloride and polymerized. After removal of acetyl groups, the polymeric product was shown by NMR spectroscopy and gel permeation chromatography to be poly(uridine 5' -p-styrenesulfonate). This uridine-containing polymer was tested against the galactosyl transferase that synthesizes lactose in the presence of alpha-lactalbumin. The polymeric compound did inhibit the enzyme strongly. And this polymer inhibited also trehalose phosphate synthase which was purified from Mycobacterium.
The adsorption of poly(uridine 5' -p-styrenesulfonate) on the polystyrene 96-well mutiplate was confirmed by ESCA measurements. On the poly(uridine 5' -p-styrene-sulfonate)-coated plate, adhesion of 3T3-L1 fibroblast which has galactosyl transferase on the cell surface was increased, compared with non-coated plate. And the increase in adhesive cell number was inhibited by the addition of EDTA, indicating that the cell adhesion needs metal ions. This result showed the 3T3-L1 fibroblast adhesion on the poly(uridine 5'-p-styrene-sulfonate)-coated plate may occur through the galactosyl transferase on the cell surface because this enzyme needs a divalent metal ion. On the other hand, Hem cell which does not have galactosyl transferase on the cell surface did not show the specific adhesion on the poly(uridine 5' -p-s tyrene-sulfonate)-coated plate. Moreover, it was suggested that the cell migration may occur by using the interaction betwecn galactosyl transferase on the cell surface of 3T3-L1 and poly(uridine 5'-p-styrene-sul fonate).

  • Research Products

    (1 results)

All Other

All Publications (1 results)

  • [Publications] 西村紳一郎, 畑中研一, 佐藤智典, 和田健彦: "生命高分子科学入門" 講談社サイエンティフィク(印刷中), (1999)

    • Description
      「研究成果報告書概要(和文)」より

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Published: 1999-12-08  

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