• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1998 Fiscal Year Final Research Report Summary

Myosin-establishing its role and location in plant cells

Research Project

Project/Area Number 08044219
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field 植物生理
Research InstitutionInternational Christian University

Principal Investigator

RIDGE Robert  International Christian University Department of Biology, Associate Professor, 教養学部, 助教授 (10211666)

Co-Investigator(Kenkyū-buntansha) SCHWARTZ O  Australian National University, Research School of Biol.Sciences, Research Fello, Research F
WILLIAMSON Re  Australian National University, Research School of Biol.Sciences, Professor, 教授
PLAZINSKI J  Australian National University, Research School of Biol.Sciences, Senior Researc, Senior Res
SHIMMEN Teruo  Himeji Institute of Technology, Department of Life Science, Professor, 理学部, 教授 (80114510)
Project Period (FY) 1996 – 1998
KeywordsMyosin / Actin / Cytoplasmic streaming / Monoclonal antibodies / Anti-sense plants / Endoplasmic reticulum / Arabidopsis / Green fluorescent protein
Research Abstract

Over the last three years we have investigated the role and location of plant myosin in plant cells. During this period we have isolated five myosin isotypes, purified many homozygous lines of anti-sense plants, and have inserted the green fluorescent protein DNA from the jellyfish Aqueorus into Arabidopsis plants. We have been able to analyze the act in-myosin-based movement of endoplasmic reticulum using the confocal laser microscope in developing cells of the root and hypocotyl. We have published a number of publications related to this work in international journals including Science, Protoplasma, Plant Physiology and Plant Cell Physiology. Currently we have submitted several papers to journals such as Plant Cell. We consider this project a large success and will continue to research this major area of basic research in plants.

  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Shimmen T.: "Studies on Mechano-Perception in Characeae : Dev't of a Monitoring Apparatus." Plant Cell Physiol.37. 591-597 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Rolfe B.G., R.W.Ridge et al.: "Defective infection and nodulation of clovers by exopolysaccharide mutants of Rhizobium leguminosarum bv.trifolii." Australian J.Plant Physiol.23. 285-303 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Collings D.A., et al.: "Actin-microtubule interactions in the alga Nitella : analysis of the mechanism by which microtubule depolymerization potentiates cytochalasin's effects on cytoplasmic streaming" Protoplasma. 191. 178-190 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Plazinski J.et al.: "Myosin from angiosperms, ferns and algae : amplification of gene fragments with PCR primers, detection of protein products with a monoclonal antibody to a conserved head epitope." Protoplasma. 196. 78-86 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tominaga, M.et al.: "Microtubules regulate the organization of actin filaments at the cortical region in root hair cells of Hydrocharis." Protoplasma. 199. 83-92 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimmen T.: "Studies on Mechano-Perception in Characeae : Pharmacological Analysis." Plant Cell Physiol.38. 139-148 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimmen T.: "Studies on Mechano-Perception in Characeae : Effects of External Ca2+ and Cl-" Plant Cell Physiol.38. 691-697 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kikuyama M., Shimmen T.: "Role of Ca2+ in Triggering Tonoplast Action Potential in Intact Nitella flexilis." Plant Cell Physiol.38. 941-944 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takabatake, R., Shimmen, T.: "Inhibition of Electrogenesis by Aluminum in Characean Cells." Plant Cell Physiol.38. 1264-1271 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimmen, T.: "Studies on Mechano-Perception in Characeae : Decrease in Electrical Membrane Resistance in Receptor Potentials." Plant Cell Physiol.38. 1298-1301 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kropf D.L.et al: "Microtubule orientation and dynamics in elongating characean internodal cells following cytosolic acidification, induction of pH bands, or premature growth arrest." Protoplasma. 197. 188-198 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arioli T.et al.: "Molecular analysis of cellulose biosynthesis in Arabidopsis thaliana." Science. 179. 717-720 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arioli T.et al.: "How many cellulose synthase-like gene products make cellulose?" Trends in Plant Science. 3. 165-166 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ridge, R.W.et al.: "The diversity of lectin-detectable sugar residues on root hair tips of selected legumes correlates with the diversity of their host ranges for rhizobia." Protoplasma. 202. 84-90 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tominaga M.et al.: "Mechanism of inhibition of cytoplasmic streaming by auxin in root hair cells of Hydrocharis." Plant Cell Physiol.39. 1342-1349 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakayasu, T.et al.: "Purification of an actin-binding protein composed of 115-kDa polypeptide from pollen tubes of lily." Biochem.Biophys.Res.Comm.249. 61-65 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokota, E.et al.: "Actin-bundling protein isolated from pollen tubes of lily. Biochemical and immunocytochemical characterization." Plant Physiol.116. 1421-1429 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokota, E.et al.: "Inhibitory regulation of higher-plant myosin by Ca2+ ions." Plant Physiol.119. 231-239 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimmen T.: "Studies on Mechano-Perception in Characeae : Development of a Monitoring Apparatus." Plant Cell Physiol.37. 591-597 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Rolfe B.G., R.W.Carlson, R.W.Ridge, F.B.Dazzo, P.F.Mateos, C.E.Pankhurst: "Defective infection and nodulation of clovers by exopolysaccharide mutants of Rhizobium leguminosarum bv.trifolii." Australian J.Plant Physiol.23. 285-303 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Collings D.A., Wasteneys G.O., Williamson R.E.: "Actin-microtubule interactions in the alga Nitella : analysis of the mechanism by which microtubule depolymerization potentiates cytochalasin's effects on cytoplasmic streaming." Protoplasma. 191. 178-190 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Plazinski J., Elliott J., Hurley U.A., Burch J., Arioli T., Williamson R.E.: "Myosin from angiosperms, ferns and algae : amplification of gene fragments with versatile PCR primers and detection of protein products with a monoclonal antibody to a conserved head epitope." Protoplasma. 196. 78-86 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tominaga, M., Morita, K., Sonobe, S., Yokota, E.and Shimmen, T.: "Microtubules regulate the organization of actin filaments at the cortical region in root hair cells of Hydrocharis." Protoplasma. 199. 83-92 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimmen, T.: "Studies on Mechano-Perception in Characeae : Pharmacological Analysis." Plant Cell Physiol.38. 139-148 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimmen, T.: "Studies on Mechano-Perception in Characeae : Effects of External Ca2+ and Cl-" Plant Cell Physiol.38. 691-697 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kikuyama M., shimmen T.: "Role of Ca2+ in Triggering Tonoplast Action Potential in Intact Nitella flexilis." Plant Cell Physiol.38. 941-944 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takabatake, R., Shimmen, T.: "Inhibition of Electrogenesis by Aluminum in Characean Cells." Plant and Cell Physiol.38. 1264-1271 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimmen, T.: "Studies on Mechano-Perception in Characeae : Decrease in Electrical Membrane Resistance in Receptor Potentials." Plant Cell Physiol.38. 1298-1301 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kropf D.L., Wasteneys G.O., Williamson R.E.: "Microtubule orientation and dynamics in elongating characean internodal cells following cytosolic acidification, induction of pH bands, or premature frowth arrest." Protoplasma. 197. 188-198 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arioli T., Peng L., Betzner A., Burn J., Wittke W., Herth W., Camilleri C., Hofte H., Plazinski J., Birch R., Cork A., Glover J., Redmond J., Williamson R.E.: "Molecular analysis of cellulose biosynthesis in Arabidopsis thaliana." Science. 179. 717-772 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arioli T., Burn J.E., Betzner A.S., Williamson R.E.: "How many cellulose synthase-like gene products make cellulose?" Trends in Plant Science. 3. 165-166 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ridge, R.W., R.Kim and F.Yoshida: "The diversity of lectin-detectable sugar residues on root hair tips of selected legumes correlates with the diversity of their host ranges for rhizobia." Protoplasma. 202. 84-90 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tominaga M., Sonobe S., Shimmen, T.: "Mechanism of inhibition of cytoplasmic streaming by auxin in root hair cells of Hydrocharis." Plant Cell Physiol.39. 1342-1349 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakayasu, T., Yokota, E.and Shimmen, T.: "Purification of an actin-binding protein composed of 115-kDa polypeptide from pollen tubes of lily." Biochem.Biophys.Res.Comm.249. 61-65 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokota, E., Takahara, K.and Shimmen, T.: "Actin-bundling protein isolated from pollen tubes of lily.Biochemical and immunocytochemical characterization." Plant Physiol.116. 1421-1429 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokota E., Muto S., Shimmen T.: "Inhibitory regulation of higher-plant myosin by Ca^<2+> ions." Plant Physiol.119. 231-239 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-12-08  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi