1997 Fiscal Year Final Research Report Summary
Molecular mechanisms of pathogenicity of rinderpestvirus and its molecular epidemio1ogy
| Project/Area Number |
08044226
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Nippon Institute for Biological Science |
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Principal Investigator |
YAMANOUCHI Kazuya Nippon Inst Bio1 Sc1, Director, 主任研究員 (30072888)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIYAMA Makoto Gifu Univ.Assoc.Prof., 農学部, 助手 (80196774)
KAMATA Hiroshi Nippon Univ.Assoc.Prof., 農獣医学部, 助手 (30139050)
KAI Chieko Tokyo Univ.Assoc.Prof., 農学部, 助教授 (10167330)
DIALLO Adama フランス熱帯獣医学研究所, 主任研究員
BARRETT Thom 英国家畜衛生研究所, 主任研究員
NUNOYA Tetsuo Nippon Inst.Biol.Sci.Director (80099994)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Rinderpest virus / Recombinant vaccine / Infection Immunity / Cell-mediated immunity / H protein / NP protein |
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| Research Abstract |
1. Studies on the efficacy of recombinant rinderpest vaccine
(1) Duration of immunity by vaccinia virus expressing the H gene of rinderpest virus
The immunity induced by the virus had already shown to last for one year. The challenge test was conducted two years after the vaccination. As a result, most of the cattle vaccinated survived the challenge except few animals, indicating the duration of immunity for two years
(2) Immune effect of vaccinia virus expressing the N gene of rinderpest virus
As the protective immunity by the virus was not shown in rabbits in the previous year, test was conducted on cattle. By the challenge with rinderpest of moderate level of virulence the cattle survived the challenge. However, the animals developed c1inical signs of rinderpest by the challenge with highly virulent rinderpest virus. These results indicate that the immunizing effect of the virus is lower than that by the vaccinia virus expressing the H gene.
2. Cell-mediated immunity in rinderpest virus infection
As the involvement of cell-mediated immunity was suggested for the vaccinia virus expressing the H gene, a method for cytotoxic test was developed by using the autologous target cells expressing the H gene. By this method appearance of cytotoxic T cells was confirmed at 1 week after the vaccination.
An involvement of specific epitope in the development of cell-mediated immunity was also indicated for the vaccinia virus expressing the NP protein.
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