1996 Fiscal Year Final Research Report Summary
Studies of protective immunity in Chages' disease-the role of immune response related genes-
| Project/Area Number |
08044241
|
|---|
| Research Category |
Grant-in-Aid for international Scientific Research
|
| Allocation Type | Single-year Grants |
|---|
| Section | Joint Research |
|---|
| Research Institution | Yamagata University |
|---|
Principal Investigator |
SAITO Susumu Yamagata University School of Medicine Associate Professor, 医学部, 助教授 (50034004)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KAMAGAWA Osami Washington University School of Medicine Asociate Professor, 医学部, 助教授
TADA Isao Kyusyu University School of Medicine Professor, 医学部, 教授 (60064531)
SENDO Fujiro Yamagata University School of Medicine Professor, 医学部, 教授 (80091833)
GRACIELA Russomando Universidad Nacional de Asuncion IICS Asociate Professor, 保健科学研究所, 主任研究員
YAMASHITA Takao Yamagata University School of Medicine Assistant, 医学部, 助手 (80018928)
|
|---|
| Project Period (FY) |
1996
|
| Keywords | Trypanosoma cruzi / Chagas' disease / cytokine / IFN-γ / South America / genetic / susceptibility / resistance |
|---|
| Research Abstract |
The susceptibility to trepanations cruzi is associated with the expansion IL-4 producing Th2 cells, whereas the resistance is correlates with the expansion of Th1 cells producing interferon γ(IFN-γ). The mechanisms operating at the initiation of Th1 or Th2 cell development form common CD4'T cell precursors after infection are not cleat. To understand the fuction of IFN-γ released from Th1, epimastigote were cultured with IFN-γ. The prolifreation of epimastigote increased by IFN-γ. Infection to LLC-M cells of tryposatigotes increased by IFN-γ.
The clinical pattern of Chagas' disease in Central America is different from that in South America; cardiac disorder is characteristic in Central America, while doth the digestive and cardiac disorders are common is South America. One of the possible causes of this differences seems to be genetic difference in T. cruzi. So far there have been few reports on the genetic differences between T. cruzi from Central America and those from South. We carried out isozyme analysis of isolates of T. cruzi obtained from Guatemala, Central America and those from South American countries, and compared the genetic structures of this parasite from both areas. Among various patterns obtained, several zymodemes genetically closely related to each other were found in Guatemalan populations, although some were found in isolates from South America, too. Two closely related zymodemes unique to South America, differed greatly from Guatemalan zymodemes. The results of this study suggest that the genetic variations might be related to the differences in pathogenicity of T. cruzi in Centraland South Americas
|
