• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1996 Fiscal Year Final Research Report Summary

Molecular mechanism of alcoholism : Ethanol and cell signaling

Research Project

Project/Area Number 08044264
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

SUZUKI Osamu  Hamamatsu University School of medicine, 医学部, 教授 (70093044)

Co-Investigator(Kenkyū-buntansha) ADRIENNE Gor  カリフォルニア大学, 神経学教室, 教授
IVAN Diamond  カリフォルニア大学, 神経学教室, 教授
浅井 清文  名古屋市立大学, 分子医学研究所, 助手 (70212462)
石井 晃  浜松医科大学, 医学部, 助手 (30252175)
妹尾 洋  浜松医科大学, 医学部, 助教授 (50236113)
Project Period (FY) 1996
Keywordsalcohol / neuronal culture cell / promotor / adenosine receptor / cAMP / protein kinase A / protein kinase C / intracellvlar distribution
Research Abstract

Alcoholism induces serious functional and pathological abnormalities in many organs, particularly in the central nervous system. However, the molecular mechanisms accounting for these effects have not been identified, because ethanol can affect many different receptors and transpoters on the cell membrane. Recent studies suggest ethanol can modify intracellular signal transduction systems. We have proposed NG108-15, neuronal cultured cells as a model, and we have found that cAMP system is involved in the induction of heterologous desensitization, an example of ethanol tolerance at a cellular level. In the present project, we have established NG108-15 cells expressing transmitter receptors by stable transfection to identify receptors of which signal transduction system could be modified by chronic ethanol.
At first, we checked the effect of chronic ethanol on promotor activity. We chose CMV,RSV and SV40 promotors and assessed the changes of these activeties using receptor binding assays … More or CAT assay. In NG108 cells transiently expressing CAT,chronic ethanol activated the activities of all promotors. However, chronic ethanol treatment did not change the expression of A_1 or D_2 receptors or CAT in some stable transfectants. These finding suggest not only promotors, but also cis elements juxtaposing with inserted cDNA would affect the effect of ethanol on the gene expresion.
In our previous study, adenosine A_2 receptor is attributed to the generation of heterologous desensitization by accumulating extracellular adenosine after chronic ethanol treatment, but A_1 receptor seems not to be involved in the induction of these phenomenon.
Protein Kinases play important roles in the intracellular signal transduction system and the translocation of these kinases is accompanied with these activation. So we focused on the translocation of protein kinases, especially A kinase (PDA) and C kinase (PKC), induced by ethanol. In NG108 cells, ethanol translocated catalytic subunit of PKA from cytosol to nucleus, but RI,a regulatory subunit of PKA was not translocated. Also, ethanol translocated some isozymes of PKC in NG108 cells. We will perform further studies on those translocations by chronic ethanol. Less

  • Research Products

    (48 results)

All Other

All Publications (48 results)

  • [Publications] O.Suzuki.,et al: "Analytical Toxicology" Forensic Science International. 80・1,2. 137-146 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kumazawa.,et al: "Reduction of pyrroloquinoline quinone by DT-diaphorase from clostridium kluyveri" Biochemical Archives. 12. 201-206 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kumazawa.,et al: "Extraction of local anaesthetics from human blood by direct immersion-solid phase micro extraction (SPME)" Chromatographia. 43・1,2. 59-62 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kumazawa.,et al: "Detection of ethanol in human body fiuids by headspace solid-phasemicro extraction (SPME) /capillary gas chromatography" Chromatographia. 43・7,8. 393-398 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Minakata.,et al: "Effect of dietary paraquat on a rat mutant unable to synthesize ascorbic acid" Arch.Toxicol.70. 256-258 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Seno.,et al: "Datection of some phenothiazines by headspace solid phase microextraction and gas chromatography" Jpn.J.Forensic Toxicol.14・1. 30-34 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Seno.,et al: "Determination of some carbamate pesticides in human body fluids by headspace solid phase micro extraction and gas chromatography" Jpn.J.Forensic Toxicol.14・3. 199-203 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Seno.,et al: "Detection of benzodiazepines in human urine by direct immersion solid phase micro extraction and gas chromatography" Jpn.J.Forensic Toxicol.15・1. 16-20 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akira.Ishii.,et al: "Simple and sensitive detection of phencyclidine in body fluids by gas chromatography with surface ionization detection" Int.J.Legal Med.108・5. 244-247 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akira.Ishii.,et al: "Sensitive quantitation of clozapine in body fluids by gas chromatography with surface ionization detection" Jpn.J.Forensic Toxicol.14・3. 215-220 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akira.Ishii.,et al: "Simple clean-up methamphetamine and amphetamine in human urineby directimmersion solid phase micro extraction (DI-SPME)" Jpn.J.Forensic Toxicol.14・3. 228-232 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akira.Ishii.,et al: "Simple extraction of phencyclidine from human body fluids by headspace solid-phase microextraction (SPME)" Chromatographia. 43・5,6. 331-334 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akira.Ishii.,et al: "A simple and sensitive quantitation of N,N-dimethyltryptamine by gas chromatography with surface ionization detection" J.Anal.Toxicol.21・1. 36-40 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] X-P.Lee.,et al: "Detection of organophosphate pesticides in human body fluids by headspace solid-phase microextraction (SPME) and capillary gas chromatography with nitrogen-phosphorus detection" Chromatographia. 42・3,4. 135-140 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Nishikawa.,et al: "The ARS-independent synthesis of DNA complementary to transfer strand in a recipient yeast during trans-Kingdomconjugation between Escherecia coli and Saccharomyces cerevisiae" Nucleic Acids Symposium Series. 35. 273-274 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Watanabe.,et al: "Simultaneous determination of cocaethylene and cocainein blood gas chromatography with surface ionization detection" Chromatographia. 44・1,2. 55-58 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] I.Isobe.,et al: "Astrocytic contributions to blood-brain barrier (BBB) formation by endothelialcells : A possible use of aortic endothelial cell for in vitro BBB model" Neurochem Int. 28・5. 759-763 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Fuse.,et al: "Heat shock-mediated cell cycle arrest is accompanied by induction of p21 CKI" Biochem.Biophys.Res.Commum. 225・3. 759-763 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Matsukawa.,et al: "Tissue distribution of human gliostatin/plateletderived endothelial cell growth factor (PD-ECGF) and its drug-induced expression" Biochemica et Biophysica Acta. 1371. 71-82 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Mizuno.,et al: "Glucocorticoid induced the expression of mRNA and the secretion of lipocortin 1 in rat astrocytoma cells" Brain Reserch. 746. 256-268 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ivan Diamond.,et al: "Cellular and molecular neuroscience of alcoholism" Physiological Reviews. 77・1. 1-20 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Douglas P.Dohrman.,et al: "Ethanol causes translocation of cAMP-dependent protein kinase catalytic subunit to nucleus" Cell Biology. 93. 10217-10221 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Imogen R.Coe.,et al: "The role of protein kinase C in cellular tolerance to ethanol" J.Biol.Chem.271・46. 29468-29474 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Imogen R.Coe.,et al: "Activation of a cyclic AMP-Dependent protein kinase reverses tolerance of nucleoside transporter to ethanol" J.Pharmacol.Exp.Therap.276・2. 365-369 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] O.Suzuki., et al: "Analytical Toxicology" Forensic Science International. 80・1,2. 137-146 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kumazawa., et al: "Reduction of pyrroloquinoline quinone by dt-diaphorase from clostridium kluyveri" Biochemical Archives. 12. 201-206 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kumazawa., et al: "Extraction of local anaesthetics from human blood by direct immersion-solid phase micro extraction (SPME)" Chromatographia. 43・1,2. 59-62 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kumazawa., et al: "Detection of ethanol in human body fluids by headspace solid-phase micro extraction (SPME)/capillary gas chromato graphy" Chromatographia. 43・7,8. 393-398 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Minakata., et al: "Effect of dietary paraquat on a rat mutant unable to synthesize ascorbic acid." Arch.Toxicol.70. 256-258 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Seno., et al: "Detection of some phenothiazines by headspace solid phase microextraction and gas chromatog-raphy" Jpn.J.Forensic Toxicol. 14・1. 30-34 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Seno., et al: "Determination of some carbamate pesticides in human body in human body fluids by headspace solid phase micro extraction and gas chromatography" Jpn.J.Forensic Toxico l. 14・3. 199-203 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Seno., et al: "Detection of benzodiazepines in human urine by direct immersion solid phase micro extraction and gas chromatography" Jpn.J.Forensic Toxicol. 15・1. 16-20 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira.Ishii., et al: "Simple and sensitive detection of phencyclidine in body fluids by gas chromatography with surface ionization detection" Int.J.Legal Med.108・5. 244-247 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira.Ishii., et al: "Sensitive zuantitation of clozapine in body fluids by gas chromatography with surface ionization detection" Jpn.J.Forensic Toxicol.14・3. 2215-220 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira.Ishii., et al: "Simple clean-up methamphetamine and amphetamine in human urine by direct-immersion solid phase micro extraction (DI-SPME)" Jpn.J.Forensic Toxicol.14・3. 228-232 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira.Ishii., et al: "Simple extraction of phencyclidine from human body fruids by headspace solid-phase microextraction (SPME)" Chromatogrsphia. 43・5,6. 331-334 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akira.Ishii., et al: "A simple and sensitive quantitation of N,N-dimethyltryptamine by gas chromatography with surface ionization detection" J.Anal.Toxicol.21・1. 36-40 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] X.-P.Lee., et al: "Detection of organophosphate pesticides in human body fluids by headspace solid-phase microextraction (SPME) and capillary gas chromatography with nitrogen-phosphorus detection" Chromatographia. 42・3,4. 135-140 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Nishikawa., et al: "ARS-independent synthesis of DNA complementary to transfer strand in arecipient yeast during trans-kingdomconjugation between Escherichia coli and Saccharomyces cerevisiae" Nucleic Acids Symposium Series, The. 273-274 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Watanabe., et al: "Simultaneous determination of cocaethylene and cocaine in blood gas chromatography with surface ionization detection" Chromatographia. 44・1,2. 55-58 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] I.Isobe., et al: "Astrocytic contributions to blood-brain barrier (BBB) formation by endothelial cells : A possible use of aortic endothelial cell for in vitro BBB model" Neurochem Int. 28・5. 523-533 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Fuse., et al: "Heat shock-mediated cell cycle arrest is accompanied by induction of p21 CKI" Biochem.Biophys.Res.Commum. 225・3. 759-763 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Matsukawa., et al: "Tissue distribution of human gliostatin/plateletderived endothelial cell growth factor (PD-ECGF) and its drug-induced expression" Biochemica et Biophysica Acta. 1371. 71-82 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Mizuno., et al: "Glucocorticoid induced the expression of mRNA and the secretion of lipocortin 1 in rat astrocytoma cells" Brain Reserch. 746. 256-268 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ivan Diamond., et al: "Cellular and molecular neuroscience of alcoholis" Physiclogical Reviews. 77・1. 1-20 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Douglas P.Dohrman., et al: "Ethanol causes translocation of cAMP-dependent protein kinase catalytic subunit to nucleus" Cell Biology. 93. 10217-10221 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imogen R.Coe., et al: "The role of protein kinase Cin cellular tolerance to ethanol" J.Biol.Chem.271,46. 29468-29274 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Imogen R.Coe., et al: "Activation of a cyclic AMP-Dependent protein kinase reverses tolerance of nucleoside transporter to ethanol" J.Pharmacol.Exp.Therap.276・2. 365-369 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-09  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi