| Research Abstract |
This project is aimed to clarify the critical signaling pathway for the regulation of cell morphology and adhesion that depends on protein-protein interactions regulated by SH2-phosphotyrosine binding or SH3-proline bindings, under the international collaborative study. Advance of recent study revealed that signaling pathway that regulates cell morphology and adhesion plays a critical role in cell growth, differentiation, organ formation and others essential for life.
Collaborators of this project, Mayr and Hanafusa have revealed that protein-protein interactions regulated by SH2-phosphotyrosine binding or SH3-proline bindings play a key role in this signaling pathway. The director of this project, Hamaguchi showed that cadherin-dependent cell adhesion was regulated by tyrosine phosphorylation. Based on these studies, Hamaguchi studied, in collaboration with Mayr and Hanafusa, the signaling pathway that regulate cadherin function, and found that c-ras playd an important role in cell-cell adhesion in v-src and v-fps transformed cells. In addition, Hamaguchi found that c-ras also playd an important role in cell-cell adhesion in v-crk transformed cells. Further studies of tyrosine phosphorylated proteins critical for cell adhesion are currently underwent by use of GST-SH2 fusion protein that offered by Mayr.
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