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1996 Fiscal Year Final Research Report Summary

Molecular cell pharmacology on Novel calcium signaling

Research Project

Project/Area Number 08044270
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionMie University

Principal Investigator

TANAKA Toshio  Mie University Faculty of Medicine, Molecular and Cellular Pharmacology. Professor and Chairman, 医学部, 教授 (00135443)

Co-Investigator(Kenkyū-buntansha) HEIZMANN C.W  Zurich University, Clinical Chemistry, Professor, 医学部, 教授
INADA Hiroyasu  Mie University Faculty. of Medicine, Molecular and cellular Pharmacology, Resear, 医学部, 助手 (90283522)
NAKA Michiko  Mie University Faculty of Medicine, Molecular and Cellular Pharmacology, Researc, 医学部, 助手 (10093139)
Project Period (FY) 1996
KeywordsS100C / S100L / Calcium-binding Proteins / Proplanolol / Differential Gene Expression / Heart Disease / Eosinophilic Chemotactic Factor / Tumor Cell
Research Abstract

The calcium signal is transmitted into the intracellular response in part via families of calcium-binding proteins which are thought to regulate a variety of biological processes. These calcium-binding protein families become of major interest because of their different expression in each disease. But it has not been reported about relation disease to S100C we first purified. we already cloned cDNA for S100C and decided the first structure of S100C.So we studied about physiological, pharmacological and pathological roles of S100C in diseases.
The cardioprotective effect of propranolol is now believed to be independent on its BETA-blocking effect and exert through the direct interaction wuth cardiac muscle, but the pathway of its action remains unclear. We found that S100C bound to propranolol and that the concentrations of propranolol for binding with S100C was similar in which propranolol was reported to exert cardioprotective effects. The concentration of propranolol was lower than those to need to interact with other calcium-binding proteins, such as calmodulin or troponin c. It sugested that S100C might be intracellular target molecule of propranolol and share other actions than B-blockade action. we also found that the gene expression of S100C was changed in the heart of myocardial infarction model rats compared with normal rats. Theses results sugest that S100C might have an important role in heart disease. And we demonstrated that S100L was a novel selective eosinophilic chemotactic factor, and had a most potent chemotactic activity on guinea pig eosinophils of all the chemotactic proteins. C.W.Heizmann found differential expression of S100L in tumor cells and normal cells and we found cell-cycle dependent differential gene expression of S100C.Thus, S100C and S100L might be thought to play an important role in many diseases.

  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Takuya Komada, et al.: "Novel Specific Chemotactic Receptor for S100L Protein on Guinea Pig Eosinophils." Biochem.Biophys.Res.Commun.220. 871-874 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaname Nakatani,et al.: "Interaction of Propranolol with S100 Proteins of the Cardiac Muscle." Eur.J.Pharmacol.315. 335-338 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田中利男 他: "カルシウムと好酸球性炎症" Clinical Calcium. 107. 9-12 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 田中利男 他: "薬理学メーリングリストの創設と活用状況" 日薬理誌Folia Pharmacol.Japan. 108. 31-35 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Beat W.Schafer et al.: "The S100 family of EF-hand calcium-binding proteins : functions and pathology" Trends in Biochemical Sciences. 21. 134-140 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Evelyn C.Ilg,et al.: "Amino Acid Sequence Determination of Human S100A12 (P6,Calgranulin C,CGRP,CAAF-1) by Tandem Mass Spectrometry" Biochem.Biophys.Res.Commun.225. 146-150 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Andrew Remppis,et al.: "Altered Expression of the Ca^<2+>-binding Proteins S100A1 in Human Cardiomyopathy" Biochemica et Biophysica Acta. 1313. 253-257 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Roland Wicki,et al.: "Characterization of the Human and Mouse cDNAs Coding for S100A13,a New member of the S100 Protein Family" Biochem.Biophys.Res. Commun. 227. 594-599 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Roland Wicki,et al.: "Characterization of the human S100A12 (calgranulin C,p6,CAAF1,CGRP) gene,a new member of the S100 gene cluster on chromosome 1q21" Cell Calcium. 20. 459-464 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Evelyn C.Ilg,et al.: "Expression Pattern of S100 Calcium-Binding Proteins in Human Tumors" Int.J.Cancer. 68. 325-332 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.Toury, et al.: "Ultrastructural Localization of α-Parvalbumin in the Epiphyseal Plate Cartilage and Bone of Growing Rats" Bone. 19. 245-253 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jorg Dreessen,et al.: "α-Parvalbumin reduces depolarization-induced elevations of cytosolic free calcium in human neuroblastoma cells" Cell Calcium. 19. 527-533 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshio Tanaka,et al.: "A SAMBROOK&TOOZE PUBLICATION AT OXFORD UNIVERSITY PRESS" Guidebook to the Calcium-binding Proteins.Edr M. R. Celio., 155-156 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takuya Komada, et al.: "Novel Specific Chemotactic Receptor for S100L Protein on Guinea Pig Eosinophils." Biochem.Biophys.Res.Commun.220. 871-874 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaname Nakatani, et al.: "Interaction of Propranolo with S100 Proteins of the Cardiac Muscle." Eur.J.Pharmacol. 315. 335-338 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshio Tanaka, et al: "Calcium and eosinophilic inflammation" Clinical Calcium. 107. 9-12 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshio Tanaka, et al: "Institution and Usefluness of pharmacological Mailing Lists" Folia Pharmacol.Japan. 108. 31-35 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Beat w.Schafer et al.: "The S100 family of EF-hand calcium-binding proteins : functions and pathology" Trends in Biochemical Sciences. 21 (4). 134-140 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Evelyn C.Ilg, et al.: "Amino Acid Sequence Determination of Human S100A12 (P6, Calgranulin C,CGRP,CAAF-1) by Tandem Mass Spectrometry" Biochem. Bio Phys, Res.commun.225 (1). 146-150 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Andrew Remppis, et al.: "Altered Expression of the ca^<2+>-binding protein S100A1 in Human Cardiomyopathy" Biochemica et Biophysica Acta. 1313. 253-257 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Roland Wicki, et al.: "Characterization of the Human and Mouse cDNAs Coding for S100Aa13, a New member of the S100 Protein Family" Biochem.Biophys.Res.Commun. 227. 594-599 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Roland Wicki, et al.: "Characterization of the human S100A12 (calgranulin c, p6, CAAF,CGRP) gene, a new member of the S100 gene cluster on chromosome 1 q21" Cell Calcium. 20. 459-464 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Evelyn C.Ilg, et al.: "Expression Pattern of S100 Calcium-Binding Proteins in Human Tumors" Int.J.Cancer. 68. 325-332 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R.Toury, et al.: "Ultrastructural Localization of alpha-Parvalbumin in the Epiphyseal Plate cartilage and Bone of Growing Rats" Bone. 19. 245-253 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jorg Dreessen, et al.: "alpha-Parvalbumin reduces depolarization-induced elevations of cytosolic free calcium in human neuroblastoma cells" Cell calcium. 19. 527-533 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshio Tanaka, et al.: "Guidebook to the Calcium-binding Proteins. Edr M.R.Celio." A SAMBROOK & TOOZE PUBLTCATION AT OXFORD UNIVERSITY PRESS. 155-156 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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