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1997 Fiscal Year Final Research Report Summary

Membrane inhibitors of complement in experimental animals

Research Project

Project/Area Number 08044311
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field Immunology
Research InstitutionNagoya City University

Principal Investigator

OKADA Hidechika  Nagoya City Univ.Sch.Med., Dep.Mol.Biol., Prof., 医学部, 教授 (30160683)

Co-Investigator(Kenkyū-buntansha) LATZKOVITS Laszlo  A.St.-Gyorgyi, Med.Univ., Neuroscience, Assoc.Prof., 助教授
MORGAN B.Paul  Univ.Wales College Med., Dep.Med.Biochem., Prof., 医学部, 教授
LAJOS Baranyi  Choju Med.Inst., Res.Fellow, 長寿医学研究所, 主任研究員 (10244542)
AKATSU Hiroyasu  Choju Med.Inst., Res.Fellow, 長寿医学研究所, 研究員
OKADA Noriko  Nagoya City Univ.Sch.Med., Dep.Mol.Biol., Asist.Prof., 医学部, 助手 (20160682)
Project Period (FY) 1996 – 1997
KeywordsComplement / Animals / DAF / MCP / CD59 / 512Ag / Crry / C5a receptor
Research Abstract

Species specitic membrane inhibitors of complement such as DAF,MCP and HRF20 (CD59) were identified in experimental animals. We have previously purified guinea pig DAF and rat 512 Ag and cloned cDNA of the molecules. Since guinea pig DAF was found to have several isotypes due to alternative splicing of mRNA,cDNA of each isotypes were transfectedinto CHO cells and the function of those geneproducts on the transfected cells were analyzed. We found that the longer the S/T rich region, the stronger the inhibitory function was observed.
We have also cloned cDNa of guinea pib MCP,mouse DAF,mouse MCP,mouse CD59, rat MCP,and rat DAF.Mouse DAFcDNA was transfectedinto CHO cells and monoclonal antibodies to mouse DAF were generated by immunizing the transfected CHO cells into hamuster and making hybridoma cells fusing the spleen cells with mouse myeloma cells. Tissue distribution of guinea pig DAF,mouse DAF and rat DAF was determined by immunostaining with monoclonal antibodies. RT-PCR methodand Western blotting methodwere also succfessfully used to determine the tissues expressing guinea pig DAF and MCP,mouse DAF,MCP and CD59 as well as rat DAF,MCP and CD59. Furthermore we have also cloned cDNA of anaphylatoxin receptors of mouse and rat.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Akatsu,H. et al.: "Unipue expression of HRF20(CD59)in human nervous tissue." Microbiol.Immunol.41. 321-330 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akatsu,H. et al.: "cDNA cloning and characterization of rat C5a anaphylatoxin receptor." Microbiol.Immunol.41. 575-580 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akahori,T. et al.: "Role of sialyl Lewisx-like epitope selectively expressed on vascular endothelial cells in lokal skin inflammation of the rat." J.Immunol.158. 5384-5392 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawamoto,Y. et al.: "The serum resistance of malaria-infected erythrocytes." Immunology,. 91. 7-12 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tada,T. et al.: "Membrane attack complex of complement and 20kDa homologous resriction facter(CD59)in myocardial infarction." Virchows Arch.430. 327-332 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naito,A. et al.: "Stability of C3 convertase in the rat classical complement pathway." Microbiol.Immunol.412. 621-624 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nonaka,M. et al.: "A new repetitive sequence uniquely present in the decay-accelerating factor genes." Immunogenetics.47. 246-255 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Farkas,I. et al.: "Neuromal C5a receptor and an associated apoptotic signal transduction pathway." J.Physiol.507. 679-687 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wang,G. et al.: "Functional defferences among multiple isoforms of guinea pig decay accelerating facter(DAF)." J.Immunol.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akatsu, H., et.al.: "Unique expression of HRF20 (CD59) in human nervous tissue." Microbiol.Immunol.41. 321-330 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akatsu, H., et.al.: "cDNA cloning and characterizationb of rat C5a anaphylatoxin receptor." Microbiol.Immunol.41. 575-580 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akahori, T., et.al.: "Role of sialyl Lewisx-like epitope selectively expressed on vascular endothelial cells in local skin inflammation of the rat." J.Immunol.158. 5384-5392 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawamoto, Y., et.al.: "The serum resistance of malaria-infected erythrocytes." Immunology. 91. 7-12 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tada, T., et.al.: "Membrane attack complex of complement and 20kDa homologous restriction factor (CD59) in myocardial infarction" Virchow Arch.430. 327-332 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naito, A.et.al.: "Stability of C3 convertase in the rat classical complement pathway." Microbiol.Immunol.412. 621-624 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nonaka, M., et.al.: "A new repetitive sequenceuniquely present in the decay-accelerating factor genes." Immunogenetics. 47. 264-255 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Farkas, I., et al.: "Neuronal C5a receptor and associatedapoptotic signal transduction pathway." J.Physiol.507. 679-687 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wang, G., et.al.: "Functional differences among multiple isoforms of guinea pig decay accelerating factor (DAF)." J.Immunol.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16   Modified: 2021-04-07  

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