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1997 Fiscal Year Final Research Report Summary

Crosstalk of immune and nervous systems by 3-D imaging

Research Project

Project/Area Number 08044312
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field Physical pharmacy
Research InstitutionNagoya City University

Principal Investigator

NAKANISHI Mamoru  Nagoya City University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (90090472)

Co-Investigator(Kenkyū-buntansha) ブレナーハセット マイケ  マクマスター大学, 健康科学部, 準教授
パデュー マリー  マクマスター大学, 健康科学部, 教授
BIENENSTOCK John  McMaster Univ., Health Sci., Professor, 健康科学部, 教授
TESHIMA Reiko  National Institute of Health Sciences, Principal Investigator, 主任研究官 (50132882)
FURUNO Tadahide  Nagoya City University, Faculty of Pharmaceutical Sciences, Assistant professor, 薬学部, 講師 (80254308)
PERDUE Mary  McMaster Univ., Health Sci., Professor
BLENNERHASSETT Michael  McMaster Univ., Health Sci., Associate Professor
Project Period (FY) 1996 – 1997
KeywordsNervous systems / Immune systems / Neurotransmitters / Mast cells / RBL-2H3 / Bradykinin / Scorpion venom / Substance P
Research Abstract

It has been well known for a long time that the mind may influence the body. Related to this, the idea of bidirectional communication between nervous and immune systems has been discussed extensively. It is based on experimental evidence showing that neurotransmitters and neuronal activity can affect immune cells and induce immunological responses. On the other hand, immune activation can influence the function of the central and autonomic nervous systems. Here, to understand the molecular mechanism of these kinds of communication between the immune and nervous systems we established a time culture model of murine symphathetic neurons co-cultured with rat basophilic leukaemia (RBL-2H3) cells (homologues of mucosal mast cells) in collaboration with Prof.John Bienenstock at McMaster University. In co-cultures of up 3 days, light microscopy identified neurite contacts with RBL-2H3 cells. Then, we have studied the calcium signals in both nerve and mast cells after the interaction by confocal laser scanning microscopy. Fluo-3 fluorescence intensity in the neurites increased after stimulation with bradykinin. this indicated that calcium ion concentration increased in the neurites with bradykinin stimulation. After that calcium ion concentration increased also in RBL-2H3 cells which were contacted with neurites. However, such kinds of calcium signals were never observed in RBL-2H3 cells which were not contacted with the neurites. Similar kinds of calcium signals were also induced in RBL-2H3 cells when the neurites were stimulated with scorpion venom. By the experiments using anti-substance P antibody, we found that substance P,which were released from the neurites, triggered these calcium signals in RBL-2H3 cells.

  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] C.Kawaura: "Atomic force microscopy for studying gene transfection mediated by cationic liposomes with a cationic cholesterol derivative." FEBS Lett.421. 69-72 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.Teshima: "Effect of an ectokinase inhibitor,K252b,on degranulation and Ca2+ signals of RBL-2H3 cells and human basophils." J.Immunol.159. 964-969 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.Okayama: "Cationic cholesterol with a hydroxyethylamino head group promotes significantly liposome-mediated gene transfection." FEBS Lett.408. 232-234 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Nakanishi: "Intracellular localization of antisense DNA transferred by cationic liposomes with a cationic cholesterol derivative." Cell Vision. 4. 128-129 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Furuno: "Surface Expresstion of CD63 Antigen(ADI Antigen)in P815 Mastocytoma Cells by Transfected IgE Receptors." Biochem.Biophys.Res.Commun.219. 740-744 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Okabe: "Confocal Fluorescence Microscopy for Antibodies against a Highly Conserved Sequence in SH2 Domains." Biochem.Biophys.Res.Commun.223. 245-249 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中西 守: "生物のスーパーセンサー" 共立出版, 186 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中西 守: "見る技術(分子・細胞のバイオイメージング)" 蛋白質核酸酵素増刊号, 350 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C.Kawaura: "Atomic force microscopy for studying gene transfection mediated by cationic liposomes with a cationic cholesterol derivative." FEBS Lett.421. 69-72 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R.Teshima: "Effect of an ectokinase inhibitor, K252b, on degranulation and Ca^<2+> signals of RBL-2H3 cells and human basophils." J.Immunol.159. 964-969 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Shimizu: "Internalization of non-permeant materials into cultured mammalian cells by vortex-stiming in the presence of a high molecular weight" Biol.Pharm.Bull. 20. 727-731 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R.Okayama: "Cationic cholesterol with a hydroxyethylamino head group promotes significantly liposome-mediated gene transfection." FEBS Lett.408. 232-234 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Nakanishi: "Intracellular localization of antisense DNA transferred by cationic liposomes with a cationic cholesterol derivative." Cell Vision. 4. 128-129 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Furuno: "Surface expression of CD63 antigen (AD1 antigen) in P815 mastocytoma cells by transfected IgE receptors." Biochem.Biophys.Res.Commun.219. 740-744 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Okabe: "Confocal fluorescence microscopy for antibodies against a highly conserved sequence in SH2 domains." Biochem.Biophys.Res.Commun.223. 245-249 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C.Torigoe: "Quantitative and realtime correlation between receptor aggregation and intracellular calcium signal transduction." Immunol.Lett.49. 169-174 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Takeuchi: "Effect of zeta potential of cationic liposomes containing cationic cholesterol derivatives on gene transfection." FEBS Lett.397. 207-209 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Nagao: "Calcium signals in helper T cells after anteraction with antigen-specific and antigen-nonspecific B cells." Biol.Pharm.Bull.19. 697-700 (1996)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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