1997 Fiscal Year Final Research Report Summary
Striatal vulnerability and function repair by neural grafts
| Project/Area Number |
08044314
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
神経・脳内生理学
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| Research Institution | Nagoya City University |
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Principal Investigator |
NISHINO Nitoo Nagoya City University Medical School, Department of Physiology, Professor, 医学部, 教授 (60073730)
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| Co-Investigator(Kenkyū-buntansha) |
BORLONGAN Ce 健康科学防衛大学, 研究員
SANBERG Paul R. University of South Florida, Department of Surgery, Professor, 医学部, 教授
MORIKAWA Shigehiro Shiga University of Medical Science, Molecular Neurobiology Research Center, Ass, 分子神経生物学研究センター, 助教授 (60220042)
INUBUSHI Toshiro Shiga University of Medical Science, Molecular Neurobiology Research Center, Pro, 分子神経生物学研究センター, 教授 (20213142)
FUKUDA Atsuo Nagoya City University Medical School, Department of Physiology, Associate profe, 医学部, 助教授 (50254272)
BORLONGAN Cesario V. Univormed Services University of the Health Sciences, Department of Anatomy and
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| Project Period (FY) |
1996 – 1997
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| Keywords | mitochondrial toxin / 3-nitropropionate / dinitrophenol / Huntington's disease / cell death / neurodegeneration / astrocytes / neural transplantation |
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| Research Abstract |
The mechanisms of the high vulnerability of the striatal neurons and glias to intoxication with metabolic inhibitors were investigated in the rat.
1. Systemic intoxication with 3-nitropropionic acid (3-NPA,a mitochondrial toxin) induced striatum selective lesions (glial necrotic cell death, dysfunction of the BBB) accompanied with Huntington's disease-like motor symptoms. Another mitochondrial toxin, 2,4-dinitrophenol (DNP), also induced selective damages in striatal neurons and vessels.
2.3-NPA and DNP have one and two-NO_2 residues in their molecule, respectively. Thus, the high vulnerability of the striatum might be due to an high sensitivity to nitrate, NO and peroxynitrite.
3. We started to analyze the variation of extracellular nitrate adn nitrite that reflect the metabolic rate of NO,using in vivo microdialysis and HPLC with electro-chemical detector.
4. Motor function and neural metworks were tried to be repaired by striatal neural transplantation.
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