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1996 Fiscal Year Final Research Report Summary

Analysis of the intracellular second messenger pathway in the intestinal epithelial cell

Research Project

Project/Area Number 08044331
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field General physiology
Research InstitutionUniversity of Shizuoka

Principal Investigator

KUWAHARA Atsukazu  Univ. of Shizuoka, 大学院, 助教授 (60142890)

Co-Investigator(Kenkyū-buntansha) SUGIYA Hiroshi  Nihon Univ. Sch., of Dentistry, 松戸歯学部, 助教授 (20050114)
MOTIZUKI Tohru  Univ. of Shizuoka, 薬学部, 助教授 (00117780)
FUJIMIYA Mineko  Shiga Univ. of Medical Sci, 医学部, 助教授 (10199359)
RECHKEMMER G.  Inst., fur Ernaehrungsphysiologie, 部長
LABURTHE M.  Inserm, Frace, 部長
Project Period (FY) 1996 – 1997
KeywordsGuinea - pig / Distal colon / Chloride secretion / Cytosolic free calcium / Tachykinin / Crypt cell / Conforcal micorscopy / Ion transport
Research Abstract

In epithelial cells of the intestine , fluid secretion is driven by the accumulation of ClィイD1-ィエD1 in the cytosol by the action of the NaィイD1+ィエD1-KィイD1+ィエD1-2ClィイD1-ィエD1 co - transporter , the NaィイD1+ィエD1-HィイD1+ィエD1 exchanger which are in the basolateral membrane . Chloride ion then enter the lumen through channels in the apical membrane and this electrogenic anion efflux is balanced by KィイD1+ィエD1 effluex through KィイD1+ィエD1 channel in the basolateral and possibly the apical membrane . Various neurpeptides including substance P control the rate of secretion by increasing the intracellular CaィイD12+ィエD1 concentration which in turn activates the ClィイD1-ィエD1channels and the KィイD1+ィエD1 channels . Substance P has been reported to activate several intracellular signal transduction system . In despite of the physiological importance of chloride secretion and its regulation by substance P in distal colon , neither the colonic epithelium signaling mechanisms nor the specific receptor present on … More these cells have been characterized .
Thus , We have examined the effect of substance P on CaィイD12+ィエD1ィイD2IィエD2 of isolated crypt cells from guinea - pig colon by confocal laser scanning microscope . Substance P , neurokinin A and neurokinin B evoked a concentration - dependent in crease in [CaィイD12+ィエD1]ィイD2iィエD2 with a rank order of potencies of substance P > neurokinin A >> neurokinin B . The selective NK-1 receptor antagonist , FK888 reduced the responses to substance P and neurokini A . In extracellular CaィイD12+ィエD1 free condition , integrated pattern of substance P - induced increase in [CaィイD12+ィエD1]ィイD2iィエD2 were significantly decreased ; substance P - induced increase in [CaィイD12+ィエD1]ィイD2IiィエD2 decreased to 50 % of control . Thapsigargin also decreased the substance P - induced increase in [CaィイD12+ィエD1]ィイD2iィエD2 to 60 % of control . Combination of exracellular CaィイD12+ィエD1 free and thapsigargin pretreatment caused a greater decrease of substance P- induced increase in [CaィイD12+ィエD1]ィイD2iィエD2 . The results suggest that both extracellular CaィイD12+ィエD1 and intracellular thapsigargin - sensitive CaィイD12+ィエD1 and intracellular thapsigargin - sensitive CaィイD12+ィエD1 stores contribute to substance P - induced increase in [CaィイD12+ィエD1]ィイD2iィエD2 . Less

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Kadowaki, M., Kuramoto, H. and Kuwahara A.: "Communication of serotonergic neurgic and nitrergic neurons to secrete electrolyes in the subnucosus plexus of the guinea-pig distal colon"Brain Research. (submitted).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagahama, M. et al.,: "Light and electronmicroscopical studies of pituitary adenylate cyclase activating peptide (PACAP) immunoreactive neurons in the enteric nervous system of rat small and large intestines"Anatomy & Embryology. 198(No.5). 341-352 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuwahara, A., Kuramoto, H. and Kadowaki, M.: "5-HT activates nitric oxide generating neurons to stimulate fluid secretion in the gut"American Journal of Physiology. 275(4). G829-G834 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujimiya, M., Yamamoto, H. and Kuwahara, A.: "Effect of VIP and PACAP on basal release of serotonin from isolated vascularly and luminally perfused rat duodenum"American Journal of Physiology. 275(4). G731-G739 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kakuyama, H., Kuwahara, A., et al.,: "Role of N-terminal active sites of galanin on neurally-evoked ciruclar muscle contractions in the guinea^pig ileum"Europeal Journal of Pharmacology. 329. 85-91 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujiyama, M., Okumiya, K. and Kuwahara, A.: "Immunoelectron microscopic demonstration of luminal release of serotonin from rat enterochromaffin cells induced by high intraluminal pressure"Histochemistry and Cell Biology. 108. 105-113 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yanaihara, N. Mochizuki, T., Kuwahara, A., et al.,: "Annals of the New York Academy of Sciences, Vol. 863"New York Academy of Sciences. 129-142 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujimiya, M., Yamamoto, I. and Kuwahara, A.: "Gut HormoneXIV"医学図書出版. 70-75 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kadowaki M., Kuramoto H., and Kuwahara A.: "Communication of serotonergic neurons and nitrergic neurons to secrete electrolytes in the submucosus plexus of the guinea - pig distal colon"Brain Research. (submitted). -1

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagahama M., Tsuzui M., Motizuki T., Iguchi K., and Kuwahara: "Light and electronmicroscopical studies of pituitary adenylate cyclase activating peptide ( PACAP ) immunoreactive neurons in the enteric nervous system of rat small and large intestines"Anatomy & Embryology. 198(No.5). 341-352 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuwahara A., Kuramoto H., and Kadowaki M.: "5-HT activates nitric oxide generating neurons to simulate fluid secretion in the gut"American Journal of Physiology. 275(4). G829-G834 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujimiya M., Yamamoto H., and Kuwahara A.: "Effect of VIP and PACAP on basal release of serotonin from isolated vascularly and luminally perfused rat duodenum"American Journal of physiology. 275(4). G731-G739 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kakuyama H., Kuwahara A., Mochizuki T., Hoshino M., and yanaihara Y.: "Role of N-terminal active sites of galanin on neurally-evoked ciruclar muscle contractions in the guinea-pig ileum"Europian Jounal of Pharmacology. 329. 85-91 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujimiya M., Okumiya K., and Kuwahara A.: "Immunoelectron microscopic demonstration of luminal release of serotonin from rat enterochromaffin cells induced by high intraluminal pressure"Histochemistry and Cell Biology. 108. 105-113 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fujimiya M., Yamamoto I., and Kuwahara A.: "Effect of increase of intraluminal pressure on release of serotonin from isolated vascularly and luminally perfused rat duodeum"Gut Hormone XIV (in Japanese). 70-75 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yanaihara N., Mochizuki T,. Kuwahara A., Hoshino M., Kakuyama H., Iguchi K., Iwanaga T., Li, J., Futai Y., Kanno T., Yamabe K., and Yanaihara C.: "Endocrine and Gastrointestinal Action of Galanin"Annales of the New York Academy of Sciences Vol. 863. 129-142 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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