1996 Fiscal Year Annual Research Report

gp34/OX40系のT細胞の増殖と癌化への関与

Research Project

Project/Area Number	08264210
Research Institution	Tokyo Medical and Dental University
Principal Investigator	中村正孝東京医科歯科大学, 疾患遺伝子実験センター, 教授 (30180392)
Co-Investigator(Kenkyū-buntansha)	田中勇悦北里大学, 理学部, 助教授 (30163588)
Keywords	HTLV-I / Tax / gp34 / OX40 / T細胞 / 細胞増殖
Research Abstract	gp34はHTLV-I感染細胞に特異的に発現する膜表面分子として同定され、我々がその遺伝子を単離した。さらに、HTLV-I Taxにより転写レベルで発現が誘導されることを明らかにしている。その後、活性化T細胞に発現するOX40と結合することが明らかとなった。gp34/OX40はTNF/TNF受容体ファミリーに属するがその機能は不明である。OX40もHTLV-I感染細胞に恒常的に発現しており、しかもHTLV-I Taxを発現している細胞のみがOX40を発現していた。これはgp34の発現と共通している。Taxの発現を誘導できるJPX-9細胞で調べたところ、OX40はTaxの発現と並行しておりIL-2受容体α鎖のTaxによる発現誘導とよく似たパターンを示した。Taxの作用は、OX40の場合もmRNAの誘導を伴うもので、転写レベルによることが明らかとなっている。gp34は正常B細胞を活性化すると発現するようになる。活性化T細胞はOX40も発現するので、両分子が結合することで免疫反応時に機能していると考えられる。HTLV-I感染T細胞ではgp34、OX40ともT細胞に発現するため、感染T細胞間で機能を発揮して異常増殖に向かう可能性がある。

Research Products

(16 results)

All Other

All Publications (16 results)

[Publications] Ohbo,K.: "Modulation of hematopoiesis in mouse with a truncated mutant of the interleukin 2 receptor γ chain." Blood. 87. 956-967 (1996)
[Publications] Saito,S.: "Expression of the interleukin-2 receptor (IL-2R) γ chain on cord blood mononuclear cells." Blood. 87. 3344-3350 (1996)
[Publications] Yanagisawa,H.: "A unique origin and multistep process for generation of expanded DRPLA triple repeats." Human Mol.Genetics. 5. 373-379 (1996)
[Publications] Higashimura,N.: "Induction of OX40,a receptor of gp34,on T cells by trans-acting transcriptional activator,Tax,of human T-cell leukemia virus typr I." Jpn.J.Cancer Res.87. 227-231 (1996)
[Publications] Sugamura,K.: "The Interleukin-2 receptor γ chain : its role in the multiple cytokine receptor complexes and T cell development in XSCID." Annu.Rev.Immunol.14. 179-205 (1996)
[Publications] Ohbo,K.: "Demonstration of a crosstalk between IL-2 and IL-5 in phosphorylation of the IL-2 receptor β chain and IL-5 receptor β chain." Int.Immunol.8. 951-960 (1996)
[Publications] Higuchi,M.: "Dispensability of Jak1 tyrosine kinase for IL-2-induced cell growth signaling in a human T cell line." Eur.J.Immunol.26. 1322-1327 (1996)
[Publications] Moffatt,S.: "A cytotoxic non-structural protein,NS-1,of human parvovirus B19 induces activation of interleukin (IL-6) gene expression." J.Virol.70. 8485-8491 (1996)
[Publications] Tanaka,N.: "Essential requirement of Itk,a T cell specific tyrosine kinase in CD2-mediated IL-2 promoter activation in human T cell line Jurkat." Eur.J.Immunol.(In press). (1997)
[Publications] Saito,S.: "Functional expression on human trophoblasts of interleukin 2 and interleukin 7 receptor complexes with the common γ chain." Biochem.Biophy.Res.Commun.(In press). (1997)
[Publications] Kariya,N.: "Protection of rabbits against HTLV-II infection with a synthetic peptide corresponding to HTLV-II neutralization region." Arch.Virol.141. 471-480 (1996)
[Publications] Imura,A.: "The human OX40/gp34 system directly mediates adhesion of activated T cells to endothelial cells." J.Exp.Med.183. 2185-2195 (1996)
[Publications] Tanaka,Y.: "Productive and lytic infection of human CD4+type 1 helper T cells with macrophage-tropic human immunodeficiency virus type 1." J.Virol.71. 465-470 (1996)
[Publications] Imura,A.: "OX40 expressed on fresh leukemic cells from ATL patients mediates cell adhesion to vascular endothelial cells : mplication for the possible involvement of the OX40 in leukemic cell infiltration." Blood.(In press). (1997)
[Publications] Koyanagi,Y.: "Induction of primary viremia and CNS invasion with HIV-I in hu-PBL-NOD-scid mouse." J.Virol.(In press). (1997)
[Publications] Nakamura,M.: "Recombinant Cell Surface Receptors" M.J.Browne (In press), (1997)

1996 Fiscal Year Annual Research Report

gp34/OX40系のT細胞の増殖と癌化への関与

Principal Investigator

中村 正孝 東京医科歯科大学, 疾患遺伝子実験センター, 教授 (30180392)

Research Products

[Publications] Ohbo,K.: "Modulation of hematopoiesis in mouse with a truncated mutant of the interleukin 2 receptor γ chain." Blood. 87. 956-967 (1996)

[Publications] Saito,S.: "Expression of the interleukin-2 receptor (IL-2R) γ chain on cord blood mononuclear cells." Blood. 87. 3344-3350 (1996)

[Publications] Yanagisawa,H.: "A unique origin and multistep process for generation of expanded DRPLA triple repeats." Human Mol.Genetics. 5. 373-379 (1996)

[Publications] Higashimura,N.: "Induction of OX40,a receptor of gp34,on T cells by trans-acting transcriptional activator,Tax,of human T-cell leukemia virus typr I." Jpn.J.Cancer Res.87. 227-231 (1996)

[Publications] Sugamura,K.: "The Interleukin-2 receptor γ chain : its role in the multiple cytokine receptor complexes and T cell development in XSCID." Annu.Rev.Immunol.14. 179-205 (1996)

[Publications] Ohbo,K.: "Demonstration of a crosstalk between IL-2 and IL-5 in phosphorylation of the IL-2 receptor β chain and IL-5 receptor β chain." Int.Immunol.8. 951-960 (1996)

[Publications] Higuchi,M.: "Dispensability of Jak1 tyrosine kinase for IL-2-induced cell growth signaling in a human T cell line." Eur.J.Immunol.26. 1322-1327 (1996)

[Publications] Moffatt,S.: "A cytotoxic non-structural protein,NS-1,of human parvovirus B19 induces activation of interleukin (IL-6) gene expression." J.Virol.70. 8485-8491 (1996)

[Publications] Tanaka,N.: "Essential requirement of Itk,a T cell specific tyrosine kinase in CD2-mediated IL-2 promoter activation in human T cell line Jurkat." Eur.J.Immunol.(In press). (1997)

[Publications] Saito,S.: "Functional expression on human trophoblasts of interleukin 2 and interleukin 7 receptor complexes with the common γ chain." Biochem.Biophy.Res.Commun.(In press). (1997)

[Publications] Kariya,N.: "Protection of rabbits against HTLV-II infection with a synthetic peptide corresponding to HTLV-II neutralization region." Arch.Virol.141. 471-480 (1996)

[Publications] Imura,A.: "The human OX40/gp34 system directly mediates adhesion of activated T cells to endothelial cells." J.Exp.Med.183. 2185-2195 (1996)

[Publications] Tanaka,Y.: "Productive and lytic infection of human CD4+type 1 helper T cells with macrophage-tropic human immunodeficiency virus type 1." J.Virol.71. 465-470 (1996)

[Publications] Imura,A.: "OX40 expressed on fresh leukemic cells from ATL patients mediates cell adhesion to vascular endothelial cells : mplication for the possible involvement of the OX40 in leukemic cell infiltration." Blood.(In press). (1997)

[Publications] Koyanagi,Y.: "Induction of primary viremia and CNS invasion with HIV-I in hu-PBL-NOD-scid mouse." J.Virol.(In press). (1997)

[Publications] Nakamura,M.: "Recombinant Cell Surface Receptors" M.J.Browne (In press), (1997)

中村正孝東京医科歯科大学, 疾患遺伝子実験センター, 教授 (30180392)