1999 Fiscal Year Annual Research Report

インターフェロン誘導6-16遺伝子の発現とその機能解析

Research Project

Project/Area Number	08265103
Research Institution	Hiroshima University
Principal Investigator	田原榮一広島大学, 医学部, 教授 (00033986)
Co-Investigator(Kenkyū-buntansha)	横崎宏広島大学, 医学部, 講師 (10200891) 安井弥広島大学, 医学部, 助教授 (40191118)
Keywords	6-16 gene / apoptosis / CIB / Bcl-2 / caspase-3 / cytochrome c / 5-FU / cycloheximide
Research Abstract	インターフェロン誘導遺伝子6-16は、細胞の老化に伴い発現が増加し、また胃癌、大腸癌の腫瘍細胞で高発現し、その上、様々なストレスによっても発現が増加してくる。一方、6-16は、サイクロヘキシミド、5-FU、無血清条件下において誘導されるアポトーシスを抑制し、その機構は、caspase-3活性に依存し、ミトコンドリアからのチトクロム_cの放出を抑制していることが明らかとなった。即ち、6-16は、ミトコンドリアに局在し、新しいミトコンドリアに関連したアポトーシス抑制蛋白質であることが明らかにされた。また6-16蛋白質は、Yeast Two-Hybrid法からCIB(Calucium-Integrin Binding Protein)と結合する。CIBは、Bcl-2と結合し、アポトーシスを促進する蛋白質である可能性があり、6-16とCIBさらにはBcl-2蛋白質ファミリーのバランスでアポトーシスを調節している可能性が考えられた。現在、6-16とCIBのノックアウトマウス、トランスジェニックマウスの作製により、個体レベルでの解析を行なっている。

Research Products
(17 results)

All Other

All Publications (17 results)

[Publications] Yasui,W.: "Immunohistochemical detection of human telomerase reverse transcriptase in normal mucosa and precancerous lesions of the stomach"Jpn J Cancer Res. 90. 589-595 (1999)
[Publications] Yokozaki,H.: "Effect of 3,4-dihydro-6-[4-(3,4-dimethoxybenzoyl)-1-piperazinyl]-2(1H)-quinolinone (vesnarinone) on the growth of gastric cancer cell lines"Cancer Lett. 140. 121-128 (1999)
[Publications] Yokozaki,H.: "Alteration p73 gene preferentially in foveolar type gastric carcinomas"Int J Cancer. 83. 192-196 (1999)
[Publications] Kuniyasu,H.: "Relative expression of E-cadherin and type IV collagenase genes predicts disease outcome in patients with resectable pancreatic carcinoma"Clin Cancer Res. 5. 25-33 (1999)
[Publications] Tahara,H.: "Immuno-histochemical detection of human telomerase catalytic component, hTERT, in human colorectal tumor and non-tumor tissue sections"Oncogene. 18. 1561-1567 (1999)
[Publications] Naka,K.: "Effect of antisense human telomerase RNA transfection on the growth of human gastric cancer cell lines"Biochem Biophys Res Commun. 255. 753-758 (1999)
[Publications] Kitadai,Y.: "Evidence for the causal role of IL-8 in angiogenesis and tumorigenecity of human gastric carcinoma cells"Br J Cancer. 81. 647-653 (1999)
[Publications] Hiyama,T.: "Overexpression of human telomerase RNA in an early event in oesophageal carcinogenesis"Virchows Arch. 434. 483-487 (1999)
[Publications] Sato,S.: "Silencing of the CD44 gene by CpG methylation in a human gastric carcinoma cell line"Jpn J Cancer Res. 90. 485-489 (1999)
[Publications] Suzuki,T.: "Expression of the E2F family in human gastrointestinal carcinomas"Int J Cancer. 81. 535-538 (1999)
[Publications] Xu,X.C.: "Expression and up-regulation of retinoic acid receptor-beta is associated with retinoid sensitivity and colony formation in esophageal cancer cell lines"Cancer Res. 59. 2477-2483 (1999)
[Publications] Yasui,W.: "Expression of cell cycle regulating transcription factor E2F-1 in colorectal carcinomas"Pathobiol. 67. 174-179 (1999)
[Publications] Nakamura,Y.: "Possible posttranscriptional regulation in cancerous and non-cancerous gastrointestinal tissues by quantitative re-evaluation of telomerase activity"Mol Carcinogenesis. 26. 312-320 (1999)
[Publications] Tahara,E.: "Molecular Pathology of Early Cancer"IOS press. 481 (1999)
[Publications] Tahara,E.: "Gastritis"Lippincott Williams & Wilkins. (1999)
[Publications] Tahara,E.: "3rd International Gastric Cancer Congress"Monduzzi Editore. 872 (1999)
[Publications] Tahara,E.: "The principles and practice of gastrointestinal oncology, Part2. Management of Specific"Oxford University Press (in press).

1999 Fiscal Year Annual Research Report

インターフェロン誘導6-16遺伝子の発現とその機能解析

Principal Investigator

田原 榮一 広島大学, 医学部, 教授 (00033986)

Research Products

[Publications] Yasui,W.: "Immunohistochemical detection of human telomerase reverse transcriptase in normal mucosa and precancerous lesions of the stomach"Jpn J Cancer Res. 90. 589-595 (1999)

[Publications] Yokozaki,H.: "Effect of 3,4-dihydro-6-[4-(3,4-dimethoxybenzoyl)-1-piperazinyl]-2(1H)-quinolinone (vesnarinone) on the growth of gastric cancer cell lines"Cancer Lett. 140. 121-128 (1999)

[Publications] Yokozaki,H.: "Alteration p73 gene preferentially in foveolar type gastric carcinomas"Int J Cancer. 83. 192-196 (1999)

[Publications] Kuniyasu,H.: "Relative expression of E-cadherin and type IV collagenase genes predicts disease outcome in patients with resectable pancreatic carcinoma"Clin Cancer Res. 5. 25-33 (1999)

[Publications] Tahara,H.: "Immuno-histochemical detection of human telomerase catalytic component, hTERT, in human colorectal tumor and non-tumor tissue sections"Oncogene. 18. 1561-1567 (1999)

[Publications] Naka,K.: "Effect of antisense human telomerase RNA transfection on the growth of human gastric cancer cell lines"Biochem Biophys Res Commun. 255. 753-758 (1999)

[Publications] Kitadai,Y.: "Evidence for the causal role of IL-8 in angiogenesis and tumorigenecity of human gastric carcinoma cells"Br J Cancer. 81. 647-653 (1999)

[Publications] Hiyama,T.: "Overexpression of human telomerase RNA in an early event in oesophageal carcinogenesis"Virchows Arch. 434. 483-487 (1999)

[Publications] Sato,S.: "Silencing of the CD44 gene by CpG methylation in a human gastric carcinoma cell line"Jpn J Cancer Res. 90. 485-489 (1999)

[Publications] Suzuki,T.: "Expression of the E2F family in human gastrointestinal carcinomas"Int J Cancer. 81. 535-538 (1999)

[Publications] Xu,X.C.: "Expression and up-regulation of retinoic acid receptor-beta is associated with retinoid sensitivity and colony formation in esophageal cancer cell lines"Cancer Res. 59. 2477-2483 (1999)

[Publications] Yasui,W.: "Expression of cell cycle regulating transcription factor E2F-1 in colorectal carcinomas"Pathobiol. 67. 174-179 (1999)

[Publications] Nakamura,Y.: "Possible posttranscriptional regulation in cancerous and non-cancerous gastrointestinal tissues by quantitative re-evaluation of telomerase activity"Mol Carcinogenesis. 26. 312-320 (1999)

[Publications] Tahara,E.: "Molecular Pathology of Early Cancer"IOS press. 481 (1999)

[Publications] Tahara,E.: "Gastritis"Lippincott Williams & Wilkins. (1999)

[Publications] Tahara,E.: "3rd International Gastric Cancer Congress"Monduzzi Editore. 872 (1999)

[Publications] Tahara,E.: "The principles and practice of gastrointestinal oncology, Part2. Management of Specific"Oxford University Press (in press).

田原榮一広島大学, 医学部, 教授 (00033986)