1997 Fiscal Year Annual Research Report

細胞複製装置:染色体の複製・分配機構

Research Project

Project/Area Number	08277103
Research Institution	Kumamoto University
Principal Investigator	平賀壯太熊本大学, 医学部, 教授 (40027321)
Co-Investigator(Kenkyū-buntansha)	吉川寛奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (70019876) 柳田充弘京都大学, 大学院・理学研究科, 教授 (80025428) 伊藤嘉明京都大学, ウイルス研究所, 教授 (80004612) 滝澤温彦大阪大学, 大学院・理学研究科, 助教授 (60154944) 杉野明雄大阪大学, 微生物病研究所, 教授 (90231737)
Keywords	DNA複製 / 染色体分配 / 複製蛋白 / 細胞分裂 / 複製起点 / ライセンス因子
Research Abstract	1.総括班会議を開催して今年度の運営方針について討議し、現在までの研究成果をまとめ平賀壯太、杉野明雄、滝澤温彦の3班長が出席して行った文部省での研究経過ヒアリングについての報告を行った。 2.各班会議にて各研究班の進展状況を把握し、評価、助言を行った。またこれらの班会議への経費の援助を行った。 3.第3回公開シンポジウム「分子細胞生物学の新しい流れ」を「細胞核の機能構造」代表/水野重樹、「生殖細胞の特質」代表/山本正幸の3重点領域研究で共催し・「3R(Replication,Recombination and Repair)シンポジウム」を「DNA修復欠損の分子病態」代表/花岡文雄の3重点領域研究と共催した。「DNA複製と染色分配のワークショップ」オ-ガナイザー:正井久雄・「第12回遺伝的組換えとその制御ワークショップ」オ-ガナイザー:大坪栄一の後援を行った。 4.総括班の松影昭夫がアメリカで開催された国際会議Cold Spring Harbor Meetingで、平賀壯太が同じくアメリカで開催されたKye Stone Symposiumでで行った研究成果発表及び、情報収集への経費を援助した。 5.サ-キュラーを発行し、総括班・計画研究班・公募研究の班員間へワークショップやシンポジウムの連絡また報告等を行い連絡をはかった。 6.今年度の計画研究班員及び、公募研究班員の研究成果をまとめ、研究成果報告書を作成し、各班員に配布した。

Research Products

(12 results)

All Other

All Publications (12 results)

[Publications] Hiraga, S.: "Cell cycle-dependent duplication and bidirectional migration of SeqA-associated DNA-protein complexes in E. coli." Mol. Cell. 1. 381-387 (1998)
[Publications] Niki, H.: "Subcellular distribution of actively partitioning F plasmid during the cell division cycle in E. coli." Cell. 90. 951-957 (1997)
[Publications] Kubota, Y.: "Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs." EMBO J.16. 3320-3331 (1997)
[Publications] Ryu, J. -R.: "Transcriptional regulation of the Drosophila raf protooncogene by the DNA replication-related element(DRE)/DRE-binding factor(DRF)system." Nucleic Acids Res.25. 794-799 (1997)
[Publications] Masai, H.: "Frpo, a novel single-stranded DNA promoter for transcription and for primer RNA synthesis of DNA replication." Cell. 89. 897-907 (1997)
[Publications] Zhang, Y. W.: "A novel transcript encoding an N-terminally truncated AML1/PEBP2 alphaB protein interferes with transactivation and blocks qranulocytic differentiation of 32Dcl3 myeloid cells." Mol. Cell Biol.17. 4133-4145 (1997)
[Publications] Nozaki, N.: "Immunoattinity puritication and characterization of CACGIG sequence binding proteins from cultured mammalian cells using an anti c-Myc monoclonal antibody recognizing the DNA binding domain." J. Biochem.121. 550-559 (1997)
[Publications] Maeda, K.: "A nalysis of an expression profile of genes in the human adipose tissue." Gene. 190. 227-235 (1997)
[Publications] Nakaseko, Y.: "A telomerase mutant defective in sister chromatidseparation at mitosis." Bioessays. 19. 557-559 (1997)
[Publications] Lei, M.: "Mcm2 is a target of regulation by Cdc7-Dbf4 during the initiation of DNA synthesis." Genes & Dev.11. 3365-3374 (1997)
[Publications] Noskov, V.: "The RFC2 gene encoding the third largest subunit of the RFC complex is required for chromosomal DNA replication and cell cycle check point in the yeast Saccharomyces cerevisiae." Mol. Cell. Biol.(in press). (1998)
[Publications] Sugino, A.: "DNA polymerase e of S. pombe encoded by cdc20_+ is required for chromosomal DNA replication." Genes to Cells.(in press). (1998)

1997 Fiscal Year Annual Research Report

細胞複製装置:染色体の複製・分配機構

Principal Investigator

平賀 壯太 熊本大学, 医学部, 教授 (40027321)

Research Products

[Publications] Hiraga, S.: "Cell cycle-dependent duplication and bidirectional migration of SeqA-associated DNA-protein complexes in E. coli." Mol. Cell. 1. 381-387 (1998)

[Publications] Niki, H.: "Subcellular distribution of actively partitioning F plasmid during the cell division cycle in E. coli." Cell. 90. 951-957 (1997)

[Publications] Kubota, Y.: "Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs." EMBO J.16. 3320-3331 (1997)

[Publications] Ryu, J. -R.: "Transcriptional regulation of the Drosophila raf protooncogene by the DNA replication-related element(DRE)/DRE-binding factor(DRF)system." Nucleic Acids Res.25. 794-799 (1997)

[Publications] Masai, H.: "Frpo, a novel single-stranded DNA promoter for transcription and for primer RNA synthesis of DNA replication." Cell. 89. 897-907 (1997)

[Publications] Zhang, Y. W.: "A novel transcript encoding an N-terminally truncated AML1/PEBP2 alphaB protein interferes with transactivation and blocks qranulocytic differentiation of 32Dcl3 myeloid cells." Mol. Cell Biol.17. 4133-4145 (1997)

[Publications] Nozaki, N.: "Immunoattinity puritication and characterization of CACGIG sequence binding proteins from cultured mammalian cells using an anti c-Myc monoclonal antibody recognizing the DNA binding domain." J. Biochem.121. 550-559 (1997)

[Publications] Maeda, K.: "A nalysis of an expression profile of genes in the human adipose tissue." Gene. 190. 227-235 (1997)

[Publications] Nakaseko, Y.: "A telomerase mutant defective in sister chromatidseparation at mitosis." Bioessays. 19. 557-559 (1997)

[Publications] Lei, M.: "Mcm2 is a target of regulation by Cdc7-Dbf4 during the initiation of DNA synthesis." Genes & Dev.11. 3365-3374 (1997)

[Publications] Noskov, V.: "The RFC2 gene encoding the third largest subunit of the RFC complex is required for chromosomal DNA replication and cell cycle check point in the yeast Saccharomyces cerevisiae." Mol. Cell. Biol.(in press). (1998)

[Publications] Sugino, A.: "DNA polymerase e of S. pombe encoded by cdc20_+ is required for chromosomal DNA replication." Genes to Cells.(in press). (1998)

平賀壯太熊本大学, 医学部, 教授 (40027321)