1998 Fiscal Year Annual Research Report

細胞複製装置:染色体の複製・分配機構

Research Project

Project/Area Number	08277103
Research Institution	Kumamoto University
Principal Investigator	平賀莊太熊本大学, 医学部, 教授 (40027321)
Co-Investigator(Kenkyū-buntansha)	吉川寛奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (70019876) 柳田充弘京都大学, 大学院・理学研究所, 教授 (80025428) 伊藤嘉明京都大学, ウイルス研究所, 教授 (80004612) 滝澤温彦大阪大学, 大学院・理学研究科, 助教授 (60154944) 杉野明雄大阪大学, 微生物病研究所, 教授 (90231737)
Keywords	DNA複製 / 染色体分配 / 複製蛋白 / 細胞分裂 / 複製起点 / ライセンス因子
Research Abstract	1. 各班会議にて各研究班の進展状況を把握し、評価、助言を行った。班会議への経費の援助を行った。 3. 東京ガーデンパレスで開催された第4回公開シンポジウム「分子細胞生物学の新しい流れ」を「細胞核の機能構造」代表/水野重樹、「生殖細胞の特質」代表/山本正幸の3重点領域研究で共催し、福岡海の中道で開催された「第14回DNA複製ワークショップ」を九州大学・薬学部・関水和久と共催した。 4. 総括班の平賀壮太がアメリカで開催された米国微生物学会の「染色体分配」で研究成果発表及び、情報収集への経費を援助した。 5. サーキュラーを発行し、総括班・計画研究班・公募研究の班員間へワークショップやシンポジウムの連絡また報告等を行い連絡をはかった。 6. 今年度の計画研究班員及び、公募研究班員の研究成果をまとめ、研究成果報告書を作成し、各班員に配付した。

Research Products

(16 results)

All Other

All Publications (16 results)

[Publications] Onogi,T.: "The assembly and migration of SeqA-Gfp fusion in living cells of Escherichia coli." Mol.Microbiol.(in press). (1999)
[Publications] Hiraga,S.: "Cell cycle-dependent duplication and bidirectional migration of SeqA-assciated DNA-protein complexes in E.coli." Mol.Cell.1. 381-387 (1998)
[Publications] Hirano,M.: "Autoregulation of the partition genes of mini-F plasmid and the intracellilar localization of their products in Escherichia coli." Mol.Gen.Genet.257. 392-403 (1998)
[Publications] Niki,H.: "Polar localization of the replication origin and terminus in Escherichia coli nucleoids during chromosome partitioning." Genes&Development. 12. 1036-1045 (1998)
[Publications] Sugino,A.: "DNA polymerase epsilon encoded by cdc20^+ is required for chromosomal DNA replication in the fission yeast Schizosaccharomyces pombe." Genes Cells. 3. 99-110 (1998)
[Publications] Noskov,V.N: "The RFC2 gene,encoding the third-largest subunit of the replication factor C complex,is required for an S-phase checkpoint in saccharomyces cervisiae." Mol Cell Biol. 18. 4914-4923 (1998)
[Publications] Maki,S.: "DNA polymerase II(epsilon)of Saccharomyces cerevisiae dissociates from the DNA template by sensing single-stranded DNA." J Biol Chem. 273. 21332-21341 (1999)
[Publications] Kamimura,Y.: "Sld2,which interacts with Dpb11 in Saccharomyces cerevisiae,is required for chromosomal DNA replication." Moi Cell Biol. 18. 6102-6109 (1998)
[Publications] Kamimura,YI: "DNA helicase III of Saccharomyces cereviase,encoded by YER176w(HEL1),highly unwinds covalently closed,circular DNA in the presence of a DNA topoismoerase and yRF-A." J Biochem. 125. 236-244 (1999)
[Publications] 0hno,K.: "cDNA cloning and expression during development of Drosophila melanogaster MCM3,MCM6 and MCM7." Gene. 217. 177-185 (1998)
[Publications] Takahashi,Y.: "Identification of three conserved regions in the DREF transcription factors from drosophila melanogaster and drosophila virilis." Nucleic Acids Res. 27. 510-516 (1999)
[Publications] Sawado,T.: "The DNA replication-related element(DRE)/DRE-binding factor system is a transcriptional regulator of the Drosophila E2F gene." J Biol Chem. 273. 26042-26051 (1998)
[Publications] Sawado,T.: "dE2F,a novel E2F-family transcription factor in Drosophila melanogaster." Biochem.Biophys.RES.Commun.251. 409-451 (1998)
[Publications] Mimura,S.: "Xenoppus Cdc45-dependent loading of DNA polymerase alpha onto chromatin under the control of S-shape cdk." EMBOJ. 17. 5699-5707 (1998)
[Publications] 滝沢温彦: "MCM/P1ファミリータンパク質とDNA複製のライセンス化" 実験医学. 16. 997-1002 (1998)
[Publications] Mizushima,Y.: "Studies on inhibitors of mammalian DNA polymerase α and β,Sulfolipids from a pteridophyte,Athyrium miponicum." Biochem.Pharmacol.55. 537-541 (1998)

1998 Fiscal Year Annual Research Report

細胞複製装置:染色体の複製・分配機構

Principal Investigator

平賀 莊太 熊本大学, 医学部, 教授 (40027321)

Research Products

[Publications] Onogi,T.: "The assembly and migration of SeqA-Gfp fusion in living cells of Escherichia coli." Mol.Microbiol.(in press). (1999)

[Publications] Hiraga,S.: "Cell cycle-dependent duplication and bidirectional migration of SeqA-assciated DNA-protein complexes in E.coli." Mol.Cell.1. 381-387 (1998)

[Publications] Hirano,M.: "Autoregulation of the partition genes of mini-F plasmid and the intracellilar localization of their products in Escherichia coli." Mol.Gen.Genet.257. 392-403 (1998)

[Publications] Niki,H.: "Polar localization of the replication origin and terminus in Escherichia coli nucleoids during chromosome partitioning." Genes&Development. 12. 1036-1045 (1998)

[Publications] Sugino,A.: "DNA polymerase epsilon encoded by cdc20^+ is required for chromosomal DNA replication in the fission yeast Schizosaccharomyces pombe." Genes Cells. 3. 99-110 (1998)

[Publications] Noskov,V.N: "The RFC2 gene,encoding the third-largest subunit of the replication factor C complex,is required for an S-phase checkpoint in saccharomyces cervisiae." Mol Cell Biol. 18. 4914-4923 (1998)

[Publications] Maki,S.: "DNA polymerase II(epsilon)of Saccharomyces cerevisiae dissociates from the DNA template by sensing single-stranded DNA." J Biol Chem. 273. 21332-21341 (1999)

[Publications] Kamimura,Y.: "Sld2,which interacts with Dpb11 in Saccharomyces cerevisiae,is required for chromosomal DNA replication." Moi Cell Biol. 18. 6102-6109 (1998)

[Publications] Kamimura,YI: "DNA helicase III of Saccharomyces cereviase,encoded by YER176w(HEL1),highly unwinds covalently closed,circular DNA in the presence of a DNA topoismoerase and yRF-A." J Biochem. 125. 236-244 (1999)

[Publications] 0hno,K.: "cDNA cloning and expression during development of Drosophila melanogaster MCM3,MCM6 and MCM7." Gene. 217. 177-185 (1998)

[Publications] Takahashi,Y.: "Identification of three conserved regions in the DREF transcription factors from drosophila melanogaster and drosophila virilis." Nucleic Acids Res. 27. 510-516 (1999)

[Publications] Sawado,T.: "The DNA replication-related element(DRE)/DRE-binding factor system is a transcriptional regulator of the Drosophila E2F gene." J Biol Chem. 273. 26042-26051 (1998)

[Publications] Sawado,T.: "dE2F,a novel E2F-family transcription factor in Drosophila melanogaster." Biochem.Biophys.RES.Commun.251. 409-451 (1998)

[Publications] Mimura,S.: "Xenoppus Cdc45-dependent loading of DNA polymerase alpha onto chromatin under the control of S-shape cdk." EMBOJ. 17. 5699-5707 (1998)

[Publications] 滝沢温彦: "MCM/P1ファミリータンパク質とDNA複製のライセンス化" 実験医学. 16. 997-1002 (1998)

[Publications] Mizushima,Y.: "Studies on inhibitors of mammalian DNA polymerase α and β,Sulfolipids from a pteridophyte,Athyrium miponicum." Biochem.Pharmacol.55. 537-541 (1998)

平賀莊太熊本大学, 医学部, 教授 (40027321)