1999 Fiscal Year Annual Research Report

細胞複製装置:染色体の複製・分配機構

Research Project

Project/Area Number	08277103
Research Institution	Kumamoto University
Principal Investigator	平賀壯太熊本大学, 医学部, 教授 (40027321)
Co-Investigator(Kenkyū-buntansha)	伊藤嘉明京都大学, ウイルス研究所, 教授 (80004612) 滝澤温彦大阪大学, 大学院・理学研究科, 教授 (60154944) 杉野明雄大阪大学, 微生物病研究所, 教授 (90231737) 吉川寛奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (70019876) 柳田充弘京都大学, 大学院・理学研究科, 教授 (80025428)
Keywords	DNA複製 / 染色体分配 / 複製蛋白 / 細胞分裂 / 複製起点 / SeqA / DnaA / 複製開始
Research Abstract	1.各班会議にて各研究班の進展状況を把握し、評価、助言を行った。 3.兵庫県・グリーンピア三木で開催された第2回"3Rシンポジウム"を特定領域(A)「DNA修復欠損の分子病態」大阪大学・花岡文男教授と共催し、大阪府・オオサカサンパレスで開催した「平成11年度文部省科学研究費補助金特定領域研究(A)公開シンポジウム」も同様に大阪大学・花岡文男教授と共催した。 4.福岡市・シーホークホテル&リゾートで開催された「第20会日本分子生物学会年会」のシンポジウム「染色体分配」(オーガナイザー:平賀壯太)に連合王国・オクスフォード大学・Jeffery Errington教授とアメリカ合衆国・ソーク研究所・Gary Karpen助教授を招待し、またErrington教授は熊本大学(平賀壯太、小椋光)を、Karpen助教授は奈良先端科学技術大学院大学(吉川寛、白髭勝彦、釣本敏樹、真木寿冶、守屋成紀)・名古屋大学(桝本寛、杉本勝則、吉田松年、小川徹、依田欣哉)・京都大学(柳田充弘、伊藤嘉明、村上洋太、大森治夫、和田千恵子)の各大学の総括班および班員の研究室を訪問してもらい研究成果の評価を受けた。 5.サーキュラーを発行し、総括班・計画研究班・公募研究の班員間へワークショップやシンポジウムの連絡また報告等を行い連絡をはかった。 6.班員間の連絡のために今年度の計画研究班員及び、公募研究班員の名簿を作成した。

Research Products

(17 results)

All Other

All Publications (17 results)

[Publications] Hiraga S.: "Bidirectional migration of SeqA-bound hemimethylated DNA clusters and pairing of oriC copies in Escherichia coli"Genes Cdlls. (in press). (2000)
[Publications] Niki H.: "Dynamic organization of chromosomal DNA in Escherichia coli"Genes Dev. 14. 212-223 (2000)
[Publications] Niki H.: "Progressing molecullar cell biology in bacteria:chromosome partitioning and cell division"蛋白質核酸酵素. 45. 156-163 (2000)
[Publications] Yagi R.: "A WW domain-containing yes-associated protein(YAP)is a novel transcriptional co-activator"EMBO J.. 18. 2551-2562 (1999)
[Publications] Yamazoe,M.: "Complex formation of MukB,MukE and MukF proteins involved in chromosome partitioning in Escherichia coli"EMBO J.. 18. 5873-5884 (1999)
[Publications] Fujita M.: "Cell cycle dependent topological changes of chromosomal replication origins in Saccharomyces cerevisiae"Genes Cells. 3. 737-749 (1999)
[Publications] Kamimura Y.I.: "DNA helicase III of Saccharomyces cerevisiae,encoded by YER176w(HEL1),highly unwinds covalently closed,circular DNA in the presence of a DNA topoisomerase and yRF-A"J Biochem(Tokyo). 125. 236-244 (1999)
[Publications] Eto K.: "Ca^<2+>/Calmodulin-dependent protein kinase cascade in Caenorhabditis elegans.Implication in transcriptional activation"J Biol Chem. 274. 22556-22562 (1999)
[Publications] Yanagida M.: "From phage to chromosome biology:a personal account."J Mol Biol. 293. 181-185 (1999)
[Publications] Esashi F: "Cdc2 phosphorylation of Crb2 is required for reestablishing cell cycle progression after the damage checkpoint"Mol Cell. 4. 167-174 (1999)
[Publications] Hirose F.: "Targeted expression of the DNA binding domain of DRE-binding factor,a Drosophila transcription factor,attenuates DNA replication of the salivary gland and eye imaginal disc"Mol Cell Biol. 19. 6020-6028 (1999)
[Publications] Kohzaki H.: "Context-dependent modulation of replication activity of saccharomyces cerevisiae autonomously replicating sequences by transcription factors"Mol Cell Biol. 19. 7428-7435 (1999)
[Publications] Niki H.: "Subcellular localization of plasmids containing the oriC region of the Escherichia coli chromosome,with or without the sopABC partitioning sytem"Mol Microbiol. 34. 498-503 (1999)
[Publications] Onogi T.: "The assembly and migration of SeqA-Gfp fusion in living cells of Escherichia coli"Mol.Microbiol. 31. 1775-1782 (1999)
[Publications] Takahashi Y.: "Identification of three conserved regions in the DREF transcription factors from Drosophila melanogaster and Drosophila virilis"Nucleic Acids Res. 27. 510-516 (1999)
[Publications] Yamaguchi M.: "Ectopic expression of human p53 inhibits entry into S phase and induces apoptosis in the Drosophila eye imaginal disc"Oncogene. 18. 6767-6775 (1999)
[Publications] Mimura S.: "Replication licensing and S-phase CDK"生化学. 71. 255-268 (1999)

1999 Fiscal Year Annual Research Report

細胞複製装置:染色体の複製・分配機構

Principal Investigator

平賀 壯太 熊本大学, 医学部, 教授 (40027321)

Research Products

[Publications] Hiraga S.: "Bidirectional migration of SeqA-bound hemimethylated DNA clusters and pairing of oriC copies in Escherichia coli"Genes Cdlls. (in press). (2000)

[Publications] Niki H.: "Dynamic organization of chromosomal DNA in Escherichia coli"Genes Dev. 14. 212-223 (2000)

[Publications] Niki H.: "Progressing molecullar cell biology in bacteria:chromosome partitioning and cell division"蛋白質核酸酵素. 45. 156-163 (2000)

[Publications] Yagi R.: "A WW domain-containing yes-associated protein(YAP)is a novel transcriptional co-activator"EMBO J.. 18. 2551-2562 (1999)

[Publications] Yamazoe,M.: "Complex formation of MukB,MukE and MukF proteins involved in chromosome partitioning in Escherichia coli"EMBO J.. 18. 5873-5884 (1999)

[Publications] Fujita M.: "Cell cycle dependent topological changes of chromosomal replication origins in Saccharomyces cerevisiae"Genes Cells. 3. 737-749 (1999)

[Publications] Kamimura Y.I.: "DNA helicase III of Saccharomyces cerevisiae,encoded by YER176w(HEL1),highly unwinds covalently closed,circular DNA in the presence of a DNA topoisomerase and yRF-A"J Biochem(Tokyo). 125. 236-244 (1999)

[Publications] Eto K.: "Ca^<2+>/Calmodulin-dependent protein kinase cascade in Caenorhabditis elegans.Implication in transcriptional activation"J Biol Chem. 274. 22556-22562 (1999)

[Publications] Yanagida M.: "From phage to chromosome biology:a personal account."J Mol Biol. 293. 181-185 (1999)

[Publications] Esashi F: "Cdc2 phosphorylation of Crb2 is required for reestablishing cell cycle progression after the damage checkpoint"Mol Cell. 4. 167-174 (1999)

[Publications] Hirose F.: "Targeted expression of the DNA binding domain of DRE-binding factor,a Drosophila transcription factor,attenuates DNA replication of the salivary gland and eye imaginal disc"Mol Cell Biol. 19. 6020-6028 (1999)

[Publications] Kohzaki H.: "Context-dependent modulation of replication activity of saccharomyces cerevisiae autonomously replicating sequences by transcription factors"Mol Cell Biol. 19. 7428-7435 (1999)

[Publications] Niki H.: "Subcellular localization of plasmids containing the oriC region of the Escherichia coli chromosome,with or without the sopABC partitioning sytem"Mol Microbiol. 34. 498-503 (1999)

[Publications] Onogi T.: "The assembly and migration of SeqA-Gfp fusion in living cells of Escherichia coli"Mol.Microbiol. 31. 1775-1782 (1999)

[Publications] Takahashi Y.: "Identification of three conserved regions in the DREF transcription factors from Drosophila melanogaster and Drosophila virilis"Nucleic Acids Res. 27. 510-516 (1999)

[Publications] Yamaguchi M.: "Ectopic expression of human p53 inhibits entry into S phase and induces apoptosis in the Drosophila eye imaginal disc"Oncogene. 18. 6767-6775 (1999)

[Publications] Mimura S.: "Replication licensing and S-phase CDK"生化学. 71. 255-268 (1999)

平賀壯太熊本大学, 医学部, 教授 (40027321)