1996 Fiscal Year Annual Research Report

アゲハの幼虫食餌選択の機構

Research Project

Project/Area Number	08304048
Section	総合
Research Institution	Chiba University
Principal Investigator	丸山工作千葉大学, 学長 (60012267)
Co-Investigator(Kenkyū-buntansha)	徳永史生大阪大学, 理学研究科, 教授 (80025452) 遠藤克彦山口大学, 理学部, 教授 (70089845) 御子柴克彦東京大学, 医科学研究科, 教授 (30051840) 神崎亮平筑波大学, 生物科学系, 講師 (40221907) 西田律夫京都大学, 農学部, 助教授 (30135545)
Keywords	産卵誘起物質 / アゲハチョウ / 昆虫の本能 / 感覚毛
Research Abstract	アゲハの感覚器官を調べるためには、まず大量のアゲハを飼育しなければならない。そのため、カラタチなどの食草を確保し、アゲハを採集し産卵させ、飼育につとめた。その結果約600個のサナギを得ることができた。そのサナギを羽化させた後、アゲハのメスのみ300羽から味覚器官である前肢フ節を切り取り、それからRNAを抽出した。前肢フ節と前肢腿節を切り取り、グアニジン塩酸存在下でホモジェナイズしたのちに抽出液をフェノール処理してRNAを分離した。約180μgの全RNAが得られた。これからmRNAを分画する予定である。mRNAをcDNAクローン化し、めざすミカン産卵誘起物質レセプターcDNAを求めるスクリーニングを試みる。そのさい、何をindexとするかが問題となる。まずは、ハエで知られている味覚レセプターの細胞膜貫通部分の塩基配列を用いてスクリーニングをおこなう予定でいる。そのほかとしては、ハエの前肢フ節の甘味感覚レセプターの探究が進んでいる。前肢フ節cDNAライブラリーから細胞膜7回貫通アミノ酸配列構造をプローブとしてクローニングした結果、いくつかのクローンが得られたので、その配列を決定し、そのどれがグルコースレセプターであるかを検討中である。困難なのは、その検定法である。目指すcDNAを培養細胞DNAに組み換え、その発現を確かめたのち、グルコースに対する応答をチェックしなければならないからである。

Research Products
(24 results)

All Other

All Publications (24 results)

[Publications] Arikawa,K.,Suyama,D.,Fujii,T.: "Light on butterfly mating." Nature. 382. 119- (1996)
[Publications] Arikawa,K.,Uchiyama,H.: "Red receptors dominate the proximal tier of the retina in the butterfly Papilio xuthus" Journal of Comparative Physiology A. 178. 55-61 (1996)
[Publications] Arikawa,K.,Miyako-Shimazaki.Y.: "Combination of physiological and anatomical methods for studying extr aocular photoreceptors on the genitalia of the butterfly,Papilio xuthus" Journal of Neuroscience Methods. 69. 75-82 (1996)
[Publications] Katayama,E.,…… & Mikoshiba,K.: "Native structure and arrangement of inositol-1,4,5-trisphosphate receptor molecules in bovine cerebellar Purkinje cells as studied by EM." The EMB Jounal. 15. 4844-4851 (1996)
[Publications] Yoshikawa,F.,…… & Mikoshiba,K.: "Mutational analysis of the ligand binding site of the inositol 1,4,5-tisphosphate receptor" J.Biol.Chem.271. 18277-18284 (1996)
[Publications] Matsumoto,M.,Nakagawa,T.et al.: "Ataxia and epileptic seizures in mice lacking type l inositol 1,4,5-tisphosphate receptor" Nature. 379. 168-171 (1996)
[Publications] Nishida,R.et al.: "Male sex pheromone of the giant danaine butterfly,Idea leuconoe." J.Chem.Ecol.22. 949-972 (1996)
[Publications] Schulz,S. & Nishida,R.: "Composition of the pheromene system of the male danaine butterfly,Idea leuconoe." Bioorg.Med.Chem.3. 341-349 (1996)
[Publications] Tan,K.H. & Nishida,R.: "Sex pheromone and mating competition after methyl eugenol consumption in the Bactrocera dorsalis complex.in B.A.MacPheron and G.J.Steck (eds.)" Fruit Fly Pests.St.Lucie,Delray Beach,Fla.147-153 (1996)
[Publications] Yokoyama,I.,Endo,K.,Yamanaka,A.,Kumagai,K.: "Species-specificity in the action of big and small prothoracicotropic hormone of the swallowtail butterfly,Papilio xuthus,P.machaon,and P.helenus." Zool.Sci.13. 449-454 (1996)
[Publications] Tanaka,D.,…… & Endo,K.: "Separation of bombyxin from a neuropeptide of Bombyx mori showing summer-morph-producing hormone activity in the Asian comma butterfly." J.Insect Physiol.(in press.). (1997)
[Publications] Endo,K.,et al.: "Stage-dependent changes of the prothoracicotropic hormone (PTTH) activity of brain extracts and of the PTTH sensitivity of the prothoracic glands." Zool.Sci.14(in press.). (1997)
[Publications] Kanzaki,R.: "Behavioral and neural basis of instinctive behavior in insects : Odor-source searching strategies without memory and learning." Robotics and Autonomus Systems. 18. 33-43 (1996)
[Publications] Okada,K.,Kanzaki,R. & Kawachi,K.: "High-speed voltage-sensitive dye imaging of an in vivo insect brain." Neurosci.Lett.209. 1-4 (1996)
[Publications] Kanzaki,R. & Mishima,T.: "Pheromone-Triggered 'Flipflopping' Neural Signals Correlated with Activities of Neck Motor Neurons of a Male Moth,Bombyx mori." Zool.Sci.13. 79-87 (1996)
[Publications] Yajima,H.,Ohtsuka,H.et al.: "Molecular cloning of a partial cDNA clone encoding the C terminal region of chicken breast muscle connectin." Zool.Sci.13. 119-123 (1996)
[Publications] Ohtani,Y.,Maki,S.,Kimura,S. & Maruyama,K.: "Localization of connectin-like proteins in leg and flight muscles of insects." Tissue & Cell. 28. 1-8 (1996)
[Publications] Yajima,H.,Ohtsuka,H.et al.: "A 11.5-kb 5′-terminal cDNA sequence of chicken breast muscle connectin/titin reveals its Z line binding region." Biochem.Biophys.Res.Commun.223. 160-164 (1996)
[Publications] Ohtsuka,H.,Yajima,H.,Maruyama & Kimura,S.: "Binding of the N-terminal 63 kDa protein of connectin/titin to α-actinin as revealed by the yeast two-hybrid system." FEBS Lett.401. 65-67 (1997)
[Publications] Hisatomi,O.,…… & Tokunaga,F.: "Molecular cloning and characterization of the putative ultraviolet-sens itive visualpigment of goldfish." Vision Res.36. 933-939 (1996)
[Publications] Kojima,D.,Ohura,T.,et al.: "Mlecular properties of chimerical of gecko blue and bovine rhod opsin." Biochemistry. 35. 2625-2629 (1996)
[Publications] Sakamoto,K.,Hisatomi,O.,Tokunaga,F. & Eguchi,E.: "Two opsins from the compound eye of the crab,Hemigrapsus sanguineus." J.Exp.Biol.199. 441-450 (1996)
[Publications] Ishikawa,M.,Tonosaki,A.,et al.: "Lectin cytochemical analysis of glycoconjugates in photoreceptor cell membranes of Lampetra japonica" Vision Res.36. 1513-1520 (1996)
[Publications] Kawamura,S.,…… & Tokunaga,F.: "Photoreceptor protein s26,a cone homologue of S-modulin in frog retina." J.Biol.Chem.271. 21359-21364 (1996)

1996 Fiscal Year Annual Research Report

アゲハの幼虫食餌選択の機構

Principal Investigator

丸山 工作 千葉大学, 学長 (60012267)

Research Products

[Publications] Arikawa,K.,Suyama,D.,Fujii,T.: "Light on butterfly mating." Nature. 382. 119- (1996)

[Publications] Arikawa,K.,Uchiyama,H.: "Red receptors dominate the proximal tier of the retina in the butterfly Papilio xuthus" Journal of Comparative Physiology A. 178. 55-61 (1996)

[Publications] Arikawa,K.,Miyako-Shimazaki.Y.: "Combination of physiological and anatomical methods for studying extr aocular photoreceptors on the genitalia of the butterfly,Papilio xuthus" Journal of Neuroscience Methods. 69. 75-82 (1996)

[Publications] Katayama,E.,…… & Mikoshiba,K.: "Native structure and arrangement of inositol-1,4,5-trisphosphate receptor molecules in bovine cerebellar Purkinje cells as studied by EM." The EMB Jounal. 15. 4844-4851 (1996)

[Publications] Yoshikawa,F.,…… & Mikoshiba,K.: "Mutational analysis of the ligand binding site of the inositol 1,4,5-tisphosphate receptor" J.Biol.Chem.271. 18277-18284 (1996)

[Publications] Matsumoto,M.,Nakagawa,T.et al.: "Ataxia and epileptic seizures in mice lacking type l inositol 1,4,5-tisphosphate receptor" Nature. 379. 168-171 (1996)

[Publications] Nishida,R.et al.: "Male sex pheromone of the giant danaine butterfly,Idea leuconoe." J.Chem.Ecol.22. 949-972 (1996)

[Publications] Schulz,S. & Nishida,R.: "Composition of the pheromene system of the male danaine butterfly,Idea leuconoe." Bioorg.Med.Chem.3. 341-349 (1996)

[Publications] Tan,K.H. & Nishida,R.: "Sex pheromone and mating competition after methyl eugenol consumption in the Bactrocera dorsalis complex.in B.A.MacPheron and G.J.Steck (eds.)" Fruit Fly Pests.St.Lucie,Delray Beach,Fla.147-153 (1996)

[Publications] Yokoyama,I.,Endo,K.,Yamanaka,A.,Kumagai,K.: "Species-specificity in the action of big and small prothoracicotropic hormone of the swallowtail butterfly,Papilio xuthus,P.machaon,and P.helenus." Zool.Sci.13. 449-454 (1996)

[Publications] Tanaka,D.,…… & Endo,K.: "Separation of bombyxin from a neuropeptide of Bombyx mori showing summer-morph-producing hormone activity in the Asian comma butterfly." J.Insect Physiol.(in press.). (1997)

[Publications] Endo,K.,et al.: "Stage-dependent changes of the prothoracicotropic hormone (PTTH) activity of brain extracts and of the PTTH sensitivity of the prothoracic glands." Zool.Sci.14(in press.). (1997)

[Publications] Kanzaki,R.: "Behavioral and neural basis of instinctive behavior in insects : Odor-source searching strategies without memory and learning." Robotics and Autonomus Systems. 18. 33-43 (1996)

[Publications] Okada,K.,Kanzaki,R. & Kawachi,K.: "High-speed voltage-sensitive dye imaging of an in vivo insect brain." Neurosci.Lett.209. 1-4 (1996)

[Publications] Kanzaki,R. & Mishima,T.: "Pheromone-Triggered 'Flipflopping' Neural Signals Correlated with Activities of Neck Motor Neurons of a Male Moth,Bombyx mori." Zool.Sci.13. 79-87 (1996)

[Publications] Yajima,H.,Ohtsuka,H.et al.: "Molecular cloning of a partial cDNA clone encoding the C terminal region of chicken breast muscle connectin." Zool.Sci.13. 119-123 (1996)

[Publications] Ohtani,Y.,Maki,S.,Kimura,S. & Maruyama,K.: "Localization of connectin-like proteins in leg and flight muscles of insects." Tissue & Cell. 28. 1-8 (1996)

[Publications] Yajima,H.,Ohtsuka,H.et al.: "A 11.5-kb 5′-terminal cDNA sequence of chicken breast muscle connectin/titin reveals its Z line binding region." Biochem.Biophys.Res.Commun.223. 160-164 (1996)

[Publications] Ohtsuka,H.,Yajima,H.,Maruyama & Kimura,S.: "Binding of the N-terminal 63 kDa protein of connectin/titin to α-actinin as revealed by the yeast two-hybrid system." FEBS Lett.401. 65-67 (1997)

[Publications] Hisatomi,O.,…… & Tokunaga,F.: "Molecular cloning and characterization of the putative ultraviolet-sens itive visualpigment of goldfish." Vision Res.36. 933-939 (1996)

[Publications] Kojima,D.,Ohura,T.,et al.: "Mlecular properties of chimerical of gecko blue and bovine rhod opsin." Biochemistry. 35. 2625-2629 (1996)

[Publications] Sakamoto,K.,Hisatomi,O.,Tokunaga,F. & Eguchi,E.: "Two opsins from the compound eye of the crab,Hemigrapsus sanguineus." J.Exp.Biol.199. 441-450 (1996)

[Publications] Ishikawa,M.,Tonosaki,A.,et al.: "Lectin cytochemical analysis of glycoconjugates in photoreceptor cell membranes of Lampetra japonica" Vision Res.36. 1513-1520 (1996)

[Publications] Kawamura,S.,…… & Tokunaga,F.: "Photoreceptor protein s26,a cone homologue of S-modulin in frog retina." J.Biol.Chem.271. 21359-21364 (1996)

丸山工作千葉大学, 学長 (60012267)