| Project/Area Number |
08304048
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
動物生理・代謝
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| Research Institution | Chiba University |
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Principal Investigator |
MARUYAMA Kosaku Chiba University, President, 学長 (60012267)
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| Co-Investigator(Kenkyū-buntansha) |
ARIKAWA Kentaro Yokohama City University, Faculty of Science, Associate Professor, 理学部, 助教授 (20167232)
ENDO Katsuhiko Yamaguchi University, Faculty of Science, Professor, 理学部, 教授 (70089845)
MIKOSHIBA Katsuhiko University of Tokyo, Institute of Medical Science, Professor, 医科学研究所, 教授 (30051840)
KANZAKI Ryohei University of Tsukuba, Institute of Biological Science, Assistant professor, 生物科学系, 講師 (40221907)
NISHIDA Ritsuo Kyoto University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (30135545)
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| Project Period (FY) |
1996 – 1997
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| Keywords | insect instinct / butterfly / ovulation stimulant / sensory fair / insect physiology |
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| Research Abstract |
Yellow sword butterfly, Papilio xuthus, lays eggs on the surface of Rutaceae leaf, after drumming the leaf surface by front leg tips. There are chemical sensory cells in the hair at the tip of the butterfly front leg.
In the present study, it was found that 5'-p-coumaroylquinic acid, epicatechin, hesperidin and hyperin induce the oviposition of the butterfly.
By use of the tip-recording technique, electric response of the sensory hairs of femal butterfly front leg to the extract of host leaves. High action potentials were produced when the extract of host leaves was applied whereas low action potentials occurred in response to the extract of non host leaves. An investigation using oviposition-inducing chemicals is under way.
A trial has been attempted to identify receptors to oviposition-inducing chemicals. Front leg tips of 500 butterflies were cut out and total RNA was extracted. By differential display method cDNAs abundant in femal legs but much less in male legs were analyzed by a DNA sequencer. One of them turned out to be p-450-cytochrome b oxidase. cDNAs corresponding to chemical receptors are being sought for.
There are light-receptive cells at the tip of the abdomen of Papilio butterfly. It was found that male light-receptive cells are necessary for copulation. On the other hand, female light-receptive cells were turned out to be needed for oviposition behavior.
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