• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1997 Fiscal Year Final Research Report Summary

Molecular analyzes of the mechanism of plant virus pathogenicity

Research Project

Project/Area Number 08306003
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 植物保護
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

FURUSAWA Iwao  Kyoto University, Guraduate School of Agriculuture, Professor, 農学研究科, 教授 (10026594)

Co-Investigator(Kenkyū-buntansha) WATANABE Yuichirou  Department of Life Science, Graduate School of Arts and Sciences, University of, 総合文化研究科生命環境科学系, 助教授 (60183125)
ISHIKAWA Masayuki  Hokkaido University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (70192482)
HOSOKAWA Daijirou  Tokyo University of Agriculuture and Technology, Professor, 農学部, 教授 (50014957)
UEDA Ichiro  Hokkaido University, Faculty of Agriculture, Professor, 農学部, 教授 (10113523)
HIBI Tadaaki  University of Tokyo, Faculty of Agriculture, Professor, 農学部, 教授 (50261954)
Project Period (FY) 1996 – 1997
KeywordsCaMV / TMV / CMV / PVX / ToMV / RNA polymerase / host factor / host specificity
Research Abstract

We have comprehensively studied the mechanism of genome replication of various plant viruses. Chronological analyzes of the accumulation of viral gene products in cauliflower mosaic virus (CaMV)-infected turnip protoplasts showed that the expression of each gene is differentially regulated. It has also been suggested that subgenomic RNAs as well as the polycistronic 35S RNA are involved in the expression of CaMV genes, indicating that CaMV uses a unique strategy for the gene expression during virus multiplication. Two domains of the 180K protein gene of tobacco mosaic virus (TMV)-OM,each containing the sequence motifs of methyltransferase or RNA polymerase, have shown to exhibit the binding activity with TMV-RNA.By immunoaffinity chromatography using antibodies specfic to the domains, heterodimers consisting of 130K and 180K viral proteins, which exhibited TMV-RNA dependent RNA synthesis in vitro, were isolated from TMV-infected tobacco leaves. We have analyzed Arabidopsis thaliana mutants designated cum1 and cum2, in which the multiplication of cucumber mosaic virsu (CMV) is delayd. Furthermore, we have been trying to clone the CUM1 gene by the positional cloning procedure. We have isolated solubilized RNA polymerase synthesizing subgenomic RNA from tobacco protoplasts inoculated with potato virus V (PVX). Amino acid 979 of the 130-and 180-kDa proteins of tomato mosaic tobamovirus (ToMV) plays an important role in overcoming the tomato Tm-1 resistance gene. Introduction of a single amino acid substitution (Gln979 to Ile) into the ToMV proteins rendered the mutant virtually unable to replicate in tomato cells while it replicated perfectly in tobacco cells, indicating the importance of the region surrounding the amino acid 979 in terms of host specificity determinants.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Kappei Kobayashi: "Accumuiation Kinetics of Viral Gene Products in Cauliflower Mosaic Virus-Infected Turnip Protoplasts" Microbiol.Immunol.42(1). 65-69 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 海道 正典: "Auto-cleavable Ribozyme Sequence Attached to Brome Mosaic Virus cDNA As Enhances Accumulation of Viral RNAs Transcribed In Vivo from the cDNAs" 日本植物病理学会報. 63(2). 95-98 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideaki Nagano: "Deletion of the C-Terminal 33 Amino Acid of Cucumber Mosaic Virus Movement Protein Enables a Chimeric Brome Mosaic Virus To Move from Cell to Cell" Journal of Virology. 71(3). 2270-2276 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kappei Kobayashi: "Accumuiation on Kinetics of Viral Gene Products in Cauliflower Mosaic Virus-Infected Turnip Protoplasts" Microbiol.Immunol.42(1). 65-69 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masanori Kaido: "Auto-cleavable Ribozyme Sequence Attached to Brome Mosaic Virus cDNA As Enhances Accumulation of Viral RNAs Transcribed In Vivo from the cDNAs" Ann.Phytopathol.Soc.Jpn.63(2). 95-98 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideaki Nagano: "Deletion of the C-Terminal 33 Amino Acids of Cucumber Mosaic Virus Movement Protein Enables a Chimeric Brome Mosaic Virus To Move from Cell to Cell" Journal of Virology. 71(3). 2270-2276 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi