光による生体内情報リズムの制御機構

1996 Fiscal Year Annual Research Report

• Project/Area Number: 08307002
• Section: 総合
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: 井端 泰彦 京都府立医科大学, 医学部, 教授 (10079684)
• Co-Investigator(Kenkyū-buntansha): 井上 慎一 山口大学, 理学部, 教授 (10274151) ; 岡村 均 神戸大学, 医学部, 教授 (60158813) ; 千原 和夫 神戸大学, 医学部, 教授 (00107955) ; 本間 さと 北海道大学, 医学部, 助教授 (20142713) ; 貴邑 冨久子 横浜市立大学, 医学部, 教授 (40046066)
• Keywords: 概日リズム / 視交叉上核 / 遺伝子 / 内分泌 / 行動 / 自律神経 / ゲッ歯類 / ヒト

Research Abstract

本研究は12名の班員による研究で、それぞれ生体リズムの発現及び同調機構、内分泌リズム、自律神経リズム、ヒトにおけるリズム発現、リズム異常、時間記憶などの研究課題について以下のような研究を行い、それぞれ成果が得られた。平成8年度は視交叉上核における光同調機構への神経回路(井端)、視交叉上核における遺伝子発現のリズムパターン(岡村)、視交叉上核にVIPニューロンによる血糖調節の自律神経機構(永井)、マウス行動パターンとそれに関与する遺伝子(井上)、マウス概日リズムの遺伝学的解析(海老原)、視交叉上核における長期増強現象のメチルコバルミンの促進効果(柴田)、視床下部GnRHサージ機構への生体時計の関与(貴邑)、視床下部成長ホルモン超日性分泌リズムの制御機構(千原)、下垂体後葉ホルモン分泌リズムに対する情動ストレスの関与(八木)、昼間光照射によるメラトニンリズムの位相変位について(本間)ヒトの生物時計特に血圧、心拍数のリズム発現について(田村)、サーカディアンリズム睡眠障害と高照度光の影響について(佐々木)。それぞれの研究成果について平成8年11月26日〜27日に関西学術研究都市けいはんなプラザにおいて班会議を行い、それぞれ研究内容について30分、討論15分の発表を行った。この班は大きく分けると生体リズムの発振体である視交叉上核に研究の主体を置いている研究者、生体における種々の生理現象のリズム解析に主体を置いている研究者のクロストークを通しての将来の研究の展望を開くのが目的であり、8年度の研究成果を踏まえ平成9年度の研究を発展させて行いたいと考える。

Research Products

• [Publications] Tanaka, M.: "Somatostatin neurons form a distinct peptidergic neuronal group in the rat suprachiasmatic nucleus : a double labeling in situ hybrldization study" Neurosci Lett.215. 119-122 (1996)
• [Publications] Kawakami, F.: "Effect of serotonergic inpts to VIPmRNA in the rat suprachiasmatic nucleus" Int. Acad. Biomed. Drug. Res.11. 161-167 (1996)
• [Publications] Tanaka, M.: "The origin of catecholaminergic innervation in the rostral ventromedial medulla oblongata of the rat" Nerosci. Lett.207. 53-56 (1996)
• [Publications] Tanaka, M.: "Induction of NGFI-A gene expression in the rat suprachiasmatic nucleus photic stimulation" Brain Res.(in press). (1997)
• [Publications] Ibata, Y.: "The suprachiasmatic nucleus : Acircadian oscillator" The Neuroscientist. (in press). (1997)
• [Publications] 井端泰彦: "生体リズムの発現及び同調機構" BME. 10, 8. 12-21 (1996)
• [Publications] Toshiya Funabashi: "Fos expression by naloxone in LHRH neurons of the mediobasal hypcthalamus and effects of pentobarbital sodivin in the proestrous rat" Jourual of Neuroendocrinology. Vol. 9. 87-92 (1997)
• [Publications] Toshiya Funabashi: "Bicuculline infusion advancs the timing of Fos expression in LHRH neurens in the preoptic area of proestrous rats" Nevro Report. Vol. 8 (in press). (1997)
• [Publications] Dai Mitsushima: "Possible role of the γ-amincbutyric acid-A receptor system in the timing of the proestrous lutenizing hormone surge in rats" Endocrinology. (in press). (1997)
• [Publications] Kinji Yagi: "A benzodiazepine, chlordiazepoxide, blocks vasopressin and oxytocin release after footshocks but not osmotic stimulus in the rat." Neuroscience Letters. 203. 49-52 (1996)
• [Publications] Kinji Yagi: "Chlordiazepoxide discriminates between the neural circuits mediating neuroendocrine responses to fear-and anxiety-producing stimli in the rat." Neuroscience Research. 24. 151-158 (1996)
• [Publications] Tatsshi Onaka: "Norepinephrine depletion impairs neuroendocrine responses to fear but not novel environmental stimuli in the rat." Brain Research. 713. 261-268 (1996)
• [Publications] Tatsushi Onaka: "A selective role of brainstem noradrenrgic neurons in oxytocin release from the neurohypophysis following noxious stimuli in the rat." Neuroscience Research. 25. 67-75 (1996)
• [Publications] Kinji Yagi: "An NMDA-receptor antagonist, MK-801 blocks the supressive vasopressin response to fear-but not to anxiety-related stimuli in the rat." Neuroscience Research. 20 (Supplement). S253- (1996)
• [Publications] Tatsushi Onaka: "Effects of noradrenaline depletion in the bed uncleus of the stria terminalis on neuroendocrine responses to fear-related stimuli in the rat." Neuroscience Research. 20 (Supplement). S254- (1996)
• [Publications] Hashimoto, S.: "Midday exposure to bright light changes the circadian organization of plasma melatonin rhythm in humans." Neurosci. Lett.(印刷中). (1997)
• [Publications] Hashimoto, S.: "Free-running circadian rhythm of melatonin in a sighted young man despite a 24-hr sleep pattern : a non-24-hour circadian syndrome." Psychiat. Clin. Nerosci.(印刷中). (1997)
• [Publications] Nakamura, K.: "A sighted man with non-24-hour sleep-wake syndrome showed damped plasma melatonin rhythm." Psychiat. Clin. Nerosci.(印刷中). (1997)
• [Publications] Nakamura, K.: "Daily melatonin intake resets circadian rhythms of a sighted man with no-24-hour sleep-wake syndrome who lacks nocturnal melatonin rise." Psychiat. Chin. Nerosci.(印刷中). (1997)
• [Publications] Honma, K.: "Sleep and melatonin rhythm in humans." Sleep and Sleep Disorders-from Molecule to Behavior. (印刷中). (1997)
• [Publications] Hashimoto, S.: "Vitamin B12 enhances the phase-response of circadian melationin rhythm to a single bright light exposure in humans." Neruscience Letters. 220. 129-132 (1996)
• [Publications] Hashimoto, S.: "Melatonin rhythm is not shifited by lights which suppress noctural melatonin in humans under entrainment." American Journal of Physiology. 270. R1073-R1077 (1996)
• [Publications] Abe. H.: "Substance P receptor regulates the photic induction of Fos-like prontein in the suprachiasmatic nucleus of Syrian hamsters." Brain Research. 708. 135-142 (1996)
• [Publications] Yoshihara, T.: "Methamphetamine stimulates the release of neuropeptide Y and noradrenaline from the paraventricular nucleus in rats." Brain Research. 707. 119-121 (1996)
• [Publications] Y. Takahashi, H. Kaji, Y. Okimura, K. Goji, H. Abe K. Chihara: "Brief Report : Short stature caused by a mutant growth hormone" The New England Journal of Medicine.334. 432-436 (1996)
• [Publications] H. Yagi, H. Kaji, M. Sato, Y. Okimura, K. Chihara: "Effect of intravenours or intracerebroventricular injections of His-D-Trp-Ala-Trp-D-Phe-Lys-NH_2 on GH release in conscios, freely moving male ra" Neuroendocrinology. 63. 198-206 (1996)
• [Publications] M. Mitani, H. Kaji. H. Abe, K. Chihara: "Growth hormone (GH) -releasing peptide and GH releasing hormone stimulate GH release from subpopulations of somatotrophs in rats" Journal of Neroendocrinology. 8. 825-830 (1996)
• [Publications] Y. Xu, H. Kaji, Y. Okimura, T. Matsui, H. Abe, K. Chihara: "Paracrine stimulation of cell growth by cholecystokinin/gastrin through cholecystokinin-B receptor on GH_3 cells in vitro" Neuroendocrinology. 64. 280-285 (1996)
• [Publications] M. Tanaka, H. Okamura, T. Matsuda, Y. Shigeyoshi, Y. Hisa, K. Chihara: "Somatostatin neurons form a distinct peptidergic neuronal group in the rat suprachiasmatic nucleus : a double in situ hybridization study" Neroscience letters. 215. 119-122 (1996)
• [Publications] A. Nagata, M. Ito, N. Iwata, J. Kuno, H. Takano, O. Minowa, K. Chihara, T. Matsui, T. Noda.: "G protein-coupled cholecystokinin-B/gastrin receptors are responsible for physilogical cell growth of the stomach mucosa in vivo" Proc. Natl. Acad. Sci. USA. 93. 11825-11830 (1996)
• [Publications] Ijiri H, Iwasaki H, Mochizuki Y, et al.: "Circadian Blood Pressre Profile and Left Ventricular Hypertrophy in Essential Hypertension." Ther. Res.17 (8). 99-104 (1996)
• [Publications] Umetani K, Tamura K, Komori, etal.: "Inhibitoly Effect of CV4151, a Thromboxane A2 Synthetase Inhibitor, on Ventricular Arrhythmias Induced by Coronary" Jpn. Cir. J.60 (6). 349-354 (1996)
• [Publications] Mochizuki Y, Okutani M, Iwasaki H. et al.: "Reproducibility of Nocturnal Blood Pressure Reduction Rate and the Prevalence of 'Non-Dippers' Using 48-Hour Ambulatory Blood Pressnre Mohitring in Patients with Essential Hypertension." Annals of The New York Academy of Sciences. 783. 330-332 (1996)
• [Publications] 望月 淳 他9名: "健常者における夜間安静期血圧および昼間活動期血圧変動に及ぼす加令の影響。" 脈管学. 36 (6). 311-325 (1996)
• [Publications] 田村康二 他2名: "冠インターベンション療法と心不全" 日本成人病学会雑誌. 22. 40- (1996)
• [Publications] Okamura H, Sugano T, Ibata Y: "Reserpine-induced immnocytochemical change of neuropeptide Y in the hypothalamic arcuate nucleus" Neurochem. Res.21. 239-243 (1996)
• [Publications] Nakagawa T, UkaiK, OhyamaT Koida M, Okamura H: "Effects of the synthesized growth hormone releasing peptide, KP-102, on growth hormone release in sodium glutamate monohydrate-treated low growth rats" Life Sci.59. 705-712 (1996)
• [Publications] Uno T, Hisa Y, Tadaki N, Okamura H, Ibata Y: "Tyrosine hydroxylase immnoreactive cells in the nodose ganglion for the canine larynx" NeuroReport. 7. 1373-1376 (1996)
• [Publications] Umehara K, Kataoka, OguraT Esumi H, Ibata Y, Okamura H: "Comparative distribtion of nitric oxide synthase (NOS) in pancreas of the dog and rat" Brain Res. Bull.(In press).
• [Publications] Yokoyama C, Okamura H: "Self-injurious behavior and dopaminergic neuron system in neonatal 6-OHDA lesioned rat. 1 Dopaminergic neurons and receptors" J. Pharmacol. Exp. Ther.(In press).
• [Publications] Okamura H. Murakami T, Yokoyama, Nakamura, Ibata Y: "Self-injurious behavior and dopaminergic neuron system in neonatal 6-OHDA lesioned rat : Intracerebral microinjection of dopamine agonists and antagonists." J. Pharmacol. Exp. Ther.(In press).
• [Publications] Kimiko Shimizu: "An immunotoxin, anti-VIP antibody-ricin A chain conjugate eliminates neurons in the hypothalamic suprachiasmatic nucleus seletively and abolishes the circadian rhth of water intake." Brain Research Bulletin. 41 (6). 369-378 (1996)
• [Publications] Katsuya Nagai: "Bilateral lesions of the hypothalamic suprachiasmatic nucleus eliminated sympathetic response to intracranial injection of 2-treoxy-D-glucose and VIP resculd this respons" Brain Research Bulletin. 39 (5). 293-297 (1996)
• [Publications] Katsuya Nagai: "SCN output derives the autonomic nervous system : with special reference to the autonomic function related to the regulation of giucose metabolism" Progress in Brain Research. 111. 253-272 (1996)
• [Publications] S. I. T. Inouye: "Circadian phythms of neuscpeptidesin the Suprachisontic nuclecs" Progress in Brain Reseerch. 111. 75-90 (1996)
• [Publications] Nishikawa, Y.: "Methylcopolamine imduces a long-lasting enhece ment of the field potontal in rat sprachiassati mucleus slies" Neuroscine Letters. 220. 199-202 (1996)
• [Publications] Hisa Y, Uno T, Tadaki N, Koike, Okamra H, Ibata Y: "Nitric oxide participation in laryngeal innervation Vocal Fold Physiology (Davis PJ and Fletcher NF eds)" Singular Publishing Group (Sandiego), 433 (1996)