1996 Fiscal Year Annual Research Report

細菌の病原性の分子遺伝学的研究

Research Project

Project/Area Number	08307004
Research Category	Grant-in-Aid for Scientific Research (A)
Section	総合
Research Institution	Yamaguchi University
Principal Investigator	中澤晶子山口大学, 医学部, 教授 (40053053)
Co-Investigator(Kenkyū-buntansha)	林英生筑波大学, 基礎医学系, 教授 (40033203) 笹川千尋東京大学, 医科学研究所, 教授 (70114494) 小熊恵二岡山大学, 医学部, 教授 (00002262) 檀原宏文北里大学, 薬学部, 教授 (40114558) 寺脇良郎信州大学, 医学部, 教授 (10014333)
Keywords	病原細菌 / 緑膿菌 / 赤痢菌Ipa蛋白 / P.gingivalis / 腸炎ビブリオ / B.fragilis / ボツリヌス毒素 / ヘリコバクター・ピロリ
Research Abstract	1.病原細菌の定着・増殖に関与する因子について以下の結果を得た。 (1)H.pyloriの尿素走化性は粘稠環境で亢進し、ウレアーゼ依存性である。 (2)縁膿菌のシデロフォア合成に関与するpvd領域は菌の生存に必須でない。 (3)腸炎ビブリオのfur遺伝子を単離し、その制御下にある遺伝子単離法を確立した。 (4)腸管凝集・粘着性大腸菌の粘着因子は16kDaの外膜蛋白である。 (5)P.gingivalisの線毛形成遺伝子はfimA領域外にも存在する。 2.病原細菌の細胞障害性因子について以下の結果を得た。 (1)黄色ブドウ球菌はアルカリショックに応答して数個の遺伝子を発現する。 (2)P.gingivalisのグリシルプロリルペプチダーゼを精製し、遺伝子の単離を開始した。 (3)B.fragilisのノイラミニダーゼ遺伝子nanHの上流の調節遺伝子nanRを同定した。 (4)C.histolyticumのコラゲナーゼ遺伝子を枯草菌に導入し本酵素精製法を確立した。 (5)ボツリヌス菌A型毒素前駆体のHAは毒素分子構成と細胞への結合・吸収に重要である。 (6)ブドウ球菌のロイコシジンとガンマヘモリジンは共通の毒素成分と固有の赤血球認識成分から構成される。 (7)緑膿菌の細胞溶解毒素は前駆体のC末が切断され活性化される。また、本酵素遺伝子を運搬するファージは緑膿菌に広く存在する。 (8)赤痢菌Ipa蛋白はα5β1インテグリンと結合し、菌の付着部位にインテグリンの集積とアクチンの重合を誘導し、細胞骨格蛋白の再構築を介して細胞内へ侵入する。 (9)サルモネラの病原遺伝子slyAはTTGを開始コドンとする。

Research Products

(27 results)

All Other

All Publications (27 results)

[Publications] Abe M.et al.: "A genetic system of Burkholderia cepacia:construction of mobilizable transposons and a cloning vector." Gene. 174. 191-194 (1996)
[Publications] Abe M.et al.: "The dsbB Gene Product Is Required for Protease Production by Burkholderia cepacia." Infect.Immun.64(10). 4378-4380 (1996)
[Publications] Mizote T.et al.: "Urease-Independent Chemotactic Responses of Helicdobacter pylori to Urea,Urease Inhibitors,and Sodium Bicarbonate." Infect.Immun.65(4)in press. (1997)
[Publications] Mizote T.et al.: "The thiJ locus and its relation to phosphorylation of hydroxymethylpyrimidine in Escherichia coli." Microbiology(UK). 142. 2969-2974 (1996)
[Publications] Yamamoto T.et al.: "Detection of the enteroaggregative Escherichia coli heat-stable enterotoxin 1 gene sequences in enterotoxigenic E.coli stains phathogenic for humans." Infect.Immun.64. 1441-1445 (1996)
[Publications] Yamamoto T.et al.: "Characterization of a novel hemagglutinin of diarrhea-associated Escherichia coli that has characteristics of diffusely adhering E.coli and enteroaggregative E.coli" Infect.Immun.64. 3694-3702 (1996)
[Publications] Yamamoto T.et al.: "Detection and sequences of the enteroaggregative Escherichia coli heat-stable enterotoxin 1 gene in enterotoxigenic E.coli strains isolated from piglets and calves with diarrhea." J.Clin.Mircobiol.35. 223-227 (1997)
[Publications] Yamamoto T.et al.: "Comparison of the nucleotide sequence of enteroaggregative Escherichia coli heatstable enterotoxin 1 genes among diarrhea-associated Escherichia coli." FEMS Microbiol.Lett.147. 89-95 (1997)
[Publications] Nakayama K.et al.: "Involvement of Arginine-Specific Cysteine Protease(Arg-gingipain) in Fimbriation of Porphyromonas gingivalis." J.Bacteriol.178(10). 2818-2824 (1996)
[Publications] Watanabe K.et al.: "Sequence and Product Analyses of the Four Genes Downstream from the Fimbrilin Gene(fimA)of the Oral Amaerobe Porphyromonas gingivalis." Microbiol.Immunol.40(10). 725-734 (1996)
[Publications] Shimizu T.et al.: "Characterization of a toxin-deficient Clostridium perfringens strain KZ1340." Microbiol.Immunol.40. 141-145 (1996)
[Publications] Ohta T.et al.: "Atomic force microscopy proposes a nevel model for stem-loop structure thatbinds a heat shock protein in the Staphylococcus aureus HSP70 operon." Biochem.Biophys.Res.Comm.226. 730-734 (1996)
[Publications] Ba-thein,W.et al: "The virR/virS locus regulates the transcription of genes encoding extracellular toxin production in Clostridium perfringens." J.Bacteriol.178. 2514-2520 (1996)
[Publications] Ohtani K.et al.: "Collagenase gene(colA)is located in the 3′-flanking region of the perfringolysin O(pfoA)Iocus in Clostridium perfringens." FEMS Microbiol.Lett.146. 155-160 (1997)
[Publications] Kuwahara T.et al.: "Detection of Bacteroides fragilis by PCR assay targeting the neuraminidase-encoding gene." Letters in Applied Microbiology. 22. 361-365 (1996)
[Publications] Jung C.M.et al.: "Expression of the colH gene encoding Clostridium histolyticum collagenase in Bacillus subtilis and its application to enzyme purification." Microbiol.Immunol.40. 923-929 (1996)
[Publications] Moriishi K.et al.: "Mosaic structures of neurotoxins produced from Clostridium botulinum type C and D organisms." Biochim.Biophys.Acta.1307(2). 123-126 (1996)
[Publications] Inoue K.et al.: "Molecular composition of Clostridium botulinum type A progenitor toxins." Infect.Immun.64(5). 1589-1594 (1996)
[Publications] Moriishi K.et al.: "Molecular cloning of the gene encoding the mosaic neurotoxin composed of parts of botulinum neurotoxin types C1 and D and PCR detection of its gene from Clostridium botulinum type Corganisms." Appl.Environ. Microbiol.62(2). 662-667 (1996)
[Publications] Takeshi K.et al.: "Simple method for detection of Clostridium botulinum type A to F neurotoxin genes by polymerase chain reaction." Microbiol.Immnuol.40(1). 5-11 (1996)
[Publications] Kameyama K.et al.: "Analysis of the phospholipase C gene of Clostridium perfringens KZ 1340 isolated from Antarctic soil." Microbiol.Immunol.40(4). 255-263 (1996)
[Publications] Yamakawa K.et al.: "Enhancement of Clostridium difficle toxin production by biotin-limited conditions." J.Med. Microbiol.44(2). 111-114 (1996)
[Publications] MacSiomoin R.et al.: "Identification and characterization of ispA,a Shigella flexneri chromosomal gene essential for normal in vivo cell division and intercellular spreading." Mol.Microbiol.19. 599-609 (1996)
[Publications] Watarai M.et al.: "Interaction of Ipa proteins of Shigella flexneri with α5β1 integrin promotes entry of the bacteria into mammalian cells." J.Exp.Med.183. 991-999 (1996)
[Publications] Uchiya K.et al.: "Identification and charactrization of phoN-Sf,a gene on the large plasmid of Shigella flexneri 2a encodind a nonspecific phosphatase." J.Bacteriol.178. 4548-4554 (1996)
[Publications] Suzuki T.et al.: "Functional analysis of Shigella VirG domains essential for interaction with vinculin and actin-based motility." J.Biol.Chem.271. 21878-21885 (1996)
[Publications] Watarai M.et al.: "rho,a small GTP-binding protein, is essential for Shigella invasion of epithelial cells." J.Exp,Med.185. 281-292 (1997)

1996 Fiscal Year Annual Research Report

細菌の病原性の分子遺伝学的研究

Principal Investigator

中澤 晶子 山口大学, 医学部, 教授 (40053053)

Research Products

[Publications] Abe M.et al.: "A genetic system of Burkholderia cepacia:construction of mobilizable transposons and a cloning vector." Gene. 174. 191-194 (1996)

[Publications] Abe M.et al.: "The dsbB Gene Product Is Required for Protease Production by Burkholderia cepacia." Infect.Immun.64(10). 4378-4380 (1996)

[Publications] Mizote T.et al.: "Urease-Independent Chemotactic Responses of Helicdobacter pylori to Urea,Urease Inhibitors,and Sodium Bicarbonate." Infect.Immun.65(4)in press. (1997)

[Publications] Mizote T.et al.: "The thiJ locus and its relation to phosphorylation of hydroxymethylpyrimidine in Escherichia coli." Microbiology(UK). 142. 2969-2974 (1996)

[Publications] Yamamoto T.et al.: "Detection of the enteroaggregative Escherichia coli heat-stable enterotoxin 1 gene sequences in enterotoxigenic E.coli stains phathogenic for humans." Infect.Immun.64. 1441-1445 (1996)

[Publications] Yamamoto T.et al.: "Characterization of a novel hemagglutinin of diarrhea-associated Escherichia coli that has characteristics of diffusely adhering E.coli and enteroaggregative E.coli" Infect.Immun.64. 3694-3702 (1996)

[Publications] Yamamoto T.et al.: "Detection and sequences of the enteroaggregative Escherichia coli heat-stable enterotoxin 1 gene in enterotoxigenic E.coli strains isolated from piglets and calves with diarrhea." J.Clin.Mircobiol.35. 223-227 (1997)

[Publications] Yamamoto T.et al.: "Comparison of the nucleotide sequence of enteroaggregative Escherichia coli heatstable enterotoxin 1 genes among diarrhea-associated Escherichia coli." FEMS Microbiol.Lett.147. 89-95 (1997)

[Publications] Nakayama K.et al.: "Involvement of Arginine-Specific Cysteine Protease(Arg-gingipain) in Fimbriation of Porphyromonas gingivalis." J.Bacteriol.178(10). 2818-2824 (1996)

[Publications] Watanabe K.et al.: "Sequence and Product Analyses of the Four Genes Downstream from the Fimbrilin Gene(fimA)of the Oral Amaerobe Porphyromonas gingivalis." Microbiol.Immunol.40(10). 725-734 (1996)

[Publications] Shimizu T.et al.: "Characterization of a toxin-deficient Clostridium perfringens strain KZ1340." Microbiol.Immunol.40. 141-145 (1996)

[Publications] Ohta T.et al.: "Atomic force microscopy proposes a nevel model for stem-loop structure thatbinds a heat shock protein in the Staphylococcus aureus HSP70 operon." Biochem.Biophys.Res.Comm.226. 730-734 (1996)

[Publications] Ba-thein,W.et al: "The virR/virS locus regulates the transcription of genes encoding extracellular toxin production in Clostridium perfringens." J.Bacteriol.178. 2514-2520 (1996)

[Publications] Ohtani K.et al.: "Collagenase gene(colA)is located in the 3′-flanking region of the perfringolysin O(pfoA)Iocus in Clostridium perfringens." FEMS Microbiol.Lett.146. 155-160 (1997)

[Publications] Kuwahara T.et al.: "Detection of Bacteroides fragilis by PCR assay targeting the neuraminidase-encoding gene." Letters in Applied Microbiology. 22. 361-365 (1996)

[Publications] Jung C.M.et al.: "Expression of the colH gene encoding Clostridium histolyticum collagenase in Bacillus subtilis and its application to enzyme purification." Microbiol.Immunol.40. 923-929 (1996)

[Publications] Moriishi K.et al.: "Mosaic structures of neurotoxins produced from Clostridium botulinum type C and D organisms." Biochim.Biophys.Acta.1307(2). 123-126 (1996)

[Publications] Inoue K.et al.: "Molecular composition of Clostridium botulinum type A progenitor toxins." Infect.Immun.64(5). 1589-1594 (1996)

[Publications] Moriishi K.et al.: "Molecular cloning of the gene encoding the mosaic neurotoxin composed of parts of botulinum neurotoxin types C1 and D and PCR detection of its gene from Clostridium botulinum type Corganisms." Appl.Environ. Microbiol.62(2). 662-667 (1996)

[Publications] Takeshi K.et al.: "Simple method for detection of Clostridium botulinum type A to F neurotoxin genes by polymerase chain reaction." Microbiol.Immnuol.40(1). 5-11 (1996)

[Publications] Kameyama K.et al.: "Analysis of the phospholipase C gene of Clostridium perfringens KZ 1340 isolated from Antarctic soil." Microbiol.Immunol.40(4). 255-263 (1996)

[Publications] Yamakawa K.et al.: "Enhancement of Clostridium difficle toxin production by biotin-limited conditions." J.Med. Microbiol.44(2). 111-114 (1996)

[Publications] MacSiomoin R.et al.: "Identification and characterization of ispA,a Shigella flexneri chromosomal gene essential for normal in vivo cell division and intercellular spreading." Mol.Microbiol.19. 599-609 (1996)

[Publications] Watarai M.et al.: "Interaction of Ipa proteins of Shigella flexneri with α5β1 integrin promotes entry of the bacteria into mammalian cells." J.Exp.Med.183. 991-999 (1996)

[Publications] Uchiya K.et al.: "Identification and charactrization of phoN-Sf,a gene on the large plasmid of Shigella flexneri 2a encodind a nonspecific phosphatase." J.Bacteriol.178. 4548-4554 (1996)

[Publications] Suzuki T.et al.: "Functional analysis of Shigella VirG domains essential for interaction with vinculin and actin-based motility." J.Biol.Chem.271. 21878-21885 (1996)

[Publications] Watarai M.et al.: "rho,a small GTP-binding protein, is essential for Shigella invasion of epithelial cells." J.Exp,Med.185. 281-292 (1997)

中澤晶子山口大学, 医学部, 教授 (40053053)