1998 Fiscal Year Annual Research Report

細菌の病原性の分子遺伝学的研究

Research Project

Project/Area Number	08307004
Research Institution	Yamaguchi University
Principal Investigator	中澤晶子山口大学, 医学部, 教授 (40053053)
Co-Investigator(Kenkyū-buntansha)	林英生筑波大学, 医学専門群, 教授 (40033203) 笹川千尋東京大学, 医科学研究所, 教授 (70114494) 小熊恵二岡山大学, 医学部, 教授 (00002262) 檀原宏文北里大学, 薬学部, 教授 (40114558) 寺脇良郎信州大学, 医学部, 教授 (10014333)
Keywords	2成分制御系 / コラゲナーゼ / 運動性 / 溶血毒 / Ipaタンパク / ビブリオゲノム / ビルレンスプラスミド / φCTXファージ
Research Abstract	1. 病原細菌の定着・増殖に関して以下の結果を得た。 1) ポルフィロモナスの2成分制御系遺伝子領域の構造を明らかにした(吉村)。 2) 腸球菌の広域バクテリオシン産生分泌遺伝子群を解析した(池)。 3) ビブリオのFur大量発現に成功し、Fur制御遺伝子の同定を行った(山本重)。 4) ヘリコバクターの胃内運動性にウレアーゼが必須であることを明らかにした(中澤)。 5) 緑膿菌のdnaJ,grpEが増殖に必須であることを証明した(津田)。 6) 黄色ブドウ球菌の熱ショック誘導遺伝子と、ウエルシュ菌のquorum sensing機構を解明した(林)。 2. 宿主障害因子に関して以下の結果を得た。 1) ガス壊疸菌は2種類のコラゲナーゼにより組織コラーゲンを分解することを明らかにした(岡部)。 2) ポルフィロモチスのペプチダーゼが病原性に関与することを認めた(吉川)。 3) バクテロイデスのノイラミニダーゼ遺伝子関連遺伝子の解析と本菌による溶血性尿毒症の症例を報告した(大西)。 4) A群レンサ球菌のNAD分解酵素遺伝子を単離した(中村)。 5) コレラ菌エルトール溶血毒のレセプターとしてグリコホリンBを同定した(本田)。 6) ボツリヌス毒素の各HA成分が小腸微絨毛にシアル酸依存的および非依存的に結合することを証明した(小熊)。 7) 黄色ブドウ球菌溶血毒の膜ポア形成における複合体構築を解明した(神尾)。 8) 赤痢菌のIpaによる細胞内シグナル伝達とVirGによるアクチン重合の誘導機構を解明した(笹川)。 9) 腸管出血性大腸菌の薬剤耐性が家畜由来である可能性を示した(山本達)。 3. ゲノムに関して以下の結果を得た。 1) 腸炎ビブリオのゲノムが2個の環状染色体より成ることを明らかにした(本田)。 2) サルモネラのビルレンスプラスミド(46kb)の塩基配列を決定した(檀原)。 3) 緑膿菌のφCTXファージの全ゲノム配列を決定し、類縁ファージを明らかにした(寺脇)。

Research Products

(50 results)

All Other

All Publications (50 results)

[Publications] Danbara,H.: "TTG as the Initiation codon of Salmonella slyA,a Gene required for Survival within Macrophages" Microbiology and Immunology. (in press). (1999)
[Publications] Hayashi,H.: "The hsp operon is repressed by the hrc37 of the hsp70 operon in Staphylococcus aureus" Microbiol.Immunol.43. 19-27 (1999)
[Publications] Hayashi,H.: "Chromosome-determined zinc responsible operon czr in Staphylococcus aureus" Microbiol.Immunol.43. 115-125 (1999)
[Publications] Honda,T.: "Physical and genetic map of the genome of Vibrio parahaemolyticus : presence of two chromosomes in Vibrio species." Mol.Microbiol.(in press). (1999)
[Publications] Honda,T.: "Disappearance of glyceraldehyde 3-phosphate dehydrogenase from erythrocyte membrane by hemolysis with thermostable direct hemolysin of vibro parahaemolyticus or V.cholerae El Tor hemolysin." Microbiol.Immunol.(in press). (1999)
[Publications] Honda,T.: "Complete nucleotide sequences of 93-kb and 3.3kb plasmids of an enterohemorrhagic Escherichia coli O157 : H7 derived from Sakai outbreak" DNA Research. 5. 1-9 (1998)
[Publications] Honda,T.: "Diagnosis of bacterial enteric infections in children in Zambia" Acta Paediatrica Japonica. 40. 259-263 (1998)
[Publications] Honda,T.: "The effectiveness of hand-disinfection by a flow water system using electrolytic products of sodium chloride,compared with a conventional method using alcoholic solution in an intensive care unit" Critical Care. 2. 79-80 (1998)
[Publications] Honda,T.: "Nicking sites in A subunit of cholera toxin and Escherichia coli heat-labile enterotoxin for Vibrio cholerae hemagglutinin/protease." Toxicon. 36. 1001-1005 (1998)
[Publications] Honda,T.: "Close proximity of the tdh,trh and ure genes on the chromosome of Vibrio parahaemolyticus." Microbiology. 144. 2517-2523 (1998)
[Publications] Honda,T.: "Profiles of circulating inflammatory-and anti-inflammatory cytokines in patients with hemolytic uremic syndrome due to E.coli O157 infection." Cytokine. 10. 544-548 (1998)
[Publications] Ike,Y.: "First report of the isolation of high-level vancomycin-resistant Enterococcus faecium from a patient in Japan" Antimicrob.Agents Chemother. 42. 2150 (1998)
[Publications] Danbara,H.: "TTG as the Initiation codon of Salmonella slyA,s Gene required for Survival within Macrophages" Microbiology and Immunology. (in press). (1999)
[Publications] Hayashi,H.: "The hsp operon is repressed by the hrc37 of the hsp70 operon in Staphylococcus aureus" Microbiol.Immunol.43. 19-27 (1999)
[Publications] Hayashi,H.: "Chromosome-determined zinc responsible operon czr in Staphylococcus aureus" Microbiol.Immunol.43. 115-125 (1999)
[Publications] Honda,T.: "Physical and genetic map of the genome of Vibrio parahaemolyticus : presence of two chromosomes in Vibrio species." Mol.Microbiol.(in press). (1999)
[Publications] Honda,T.: "Disappearance of glyceraldehyde 3-phosphate dehydrogenase from erythrocyte membrane by hemolysis with thermostable direct hemolysin of Vibrio parahaemolyticus or V.cholerae El T or hemolysin." Microbiol.Immunol.(in press). (1999)
[Publications] Honda,T.: "Complete nucleotide sequences of 93-kb and 3.3kb plasmids of an enterohemorrhagic Escherichia coli O157 : H7 derived from Sakai outbreak" DNA Research. 5. 1-9 (1998)
[Publications] Honda,T.: "Diagnosis of bacterial enteric infections in children in Zambia" Acta Paediatrica Japonica. 40. 259-263 (1998)
[Publications] Honda,T.: "The effectiveness of hand-disinfection by a flow water system using electrolytic products of sodium chloride,compared with a conventional method using alcoholic solution in an intensive care unit" Critical Care. 2. 79-80 (1998)
[Publications] Honda,T.: "Nicking sites in A subunit of cholera toxin and Escherichia coli heat-labile enterotoxin for Vibrio cholerae hemagglutinin/protease." Toxicon. 36. 1001-1005 (1998)
[Publications] Honda,T.: "Close proximity of the tdh,trh and ure genes on the chromosome of Vibrio parahaemolyticus." Microbiology. 144. 2517-2523 (1998)
[Publications] Honda,T.: "Profiles of circulating inflammatory-and anti-inflammatory cytokines in patients with hemolytic uremic syndrome due to E.coli O157 infection." Cytokine. 10. 544-548 (1998)
[Publications] Ike,Y: "First report of the isolation of high-level vancomycin-resistant Enterococcus faecium from a patient in Japan" Antimicrob.Agents Chemother. 42. 2150 (1998)
[Publications] Ike,Y.: "Evidence of nosocomial Infection in Japan caused by high-level gentamicin-resistant Enterococcus faecalis and identification of the pheromone-responsive conjugative plasmid encoding gentamicin resistance" J.Clin.Microbiol.36. 2460-2464 (1998)
[Publications] Ike,Y.: "Efficient transfer of the pheromone-independent Enterococcus faecium plasmid pMG1 (Gm′)(65.1 Kirobases)to Enterococcus strains during broth mating" J.Bacteriol.180. 4886-4892 (1998)
[Publications] Kamio,Y.: "Complete nucleotide sequence and molecular characterization of the temperate stapylococcal bacteriopahge phi-PVL carrying Panton-Valentine leukocidin genes" Gene. 215. 57-67 (1998)
[Publications] Kamio,Y.: "An N-terminal region of LukF of Staphylococcal leukocidin/gamma-hemolysin crucial for the biologica activity of the toxin" Biosci.Biotechnol.Biochem.62(7). 1465-1467 (1998)
[Publications] Kamio,Y.: "Further study on the two pivotal parts of Hlg2 for the full hemolytic activity of staphylococcal gamma-hemolysin" Biosci.Biotechnol.Biochem.62(9). 1745-1750 (1998)
[Publications] Kamio,Y.: "Phosphorylation of LukS by protein kinase A is crucial for the LukS-specific function of the staphylococcal leukocidin on human polymorphonuclear leukocytes" Biosci Biotechnol.Biochem.62(9). 1834-1838 (1998)
[Publications] Kamio,Y.: "Crystal structure of staphylococcal LukF delineates conformational changes accompaning formation of a transmembrane channel" Nature(Structural Biology). 6(2). 6-12 (1998)
[Publications] Nakazawa,T.: "Persistent infection by Helicobacter pylori Down-regulates virus-specific CD8+ cytotoxic T cell response and prolongs viral infection" J Infect Dis. 177. 72-80 (1998)
[Publications] Nakazawa,T.: "Urease plays an important role in the chemotactic motility of Helicobacter pylori in a viscous environment" Infect.Immun.66. 4832-4837 (1998)
[Publications] Nakazawa,T.: "Nucleotide sequence and characterization of cdrA,a cell division-related gene of Helicobacter pylori" J.Bacteriol.180. 5263-5268 (1998)
[Publications] Nakazawa,T.: "Chemotaxis of Helicobacter pylori : a urease-independent response" J.Gastroenterol.33(S). 1-5 (1998)
[Publications] Oguma,K.: "botR/A is a positive regulator of botulinum neurotoxin and associated non-toxin protein genes in Clostridium botulinum A" Mol.Microbiol.29. 1009-1018 (1998)
[Publications] Oguma,K.: "Molecular composition of the 16S toxin produced by a Clostridium botulinum type D strain,1873" Microbiol.Immunol.42. 599-605 (1998)
[Publications] Oguma,K.: "Classification of Clostridium botulinum based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and pulsed-field gel electrophoresis" Anaerobe. 4. (1998)
[Publications] Ohnishi,Y.: "Culture supernatants of Lactobacillus acidophilus and Bifidobacterium adolescentis repress ileal ulcer formation in rats treated with a nonsteroidal antiin flamatory drug by supressing unbalanced growth of aerobic bacteria and lipid peroxidation" Microbiol.Immunol.42. 347-355 (1998)
[Publications] Ohnishi,Y.: "Prostective effects of a culture supernatant of Lactobacillus acidophilus and antioxidants on ileal ulcer formation in rats treated with a nonsteroideal antiinflamatory drug" Microbiol.Immunol.42. 745-753 (1998)
[Publications] Ohnishi,Y.: "Hemolytic uremic syndrome in a patient witth Bacteroides fragilis infection" J.Jap.Assoc.Inf.Dis.72. 162-166 (1998)
[Publications] Okabe,A.: "Gene duplication and multiplicity of collagenases in Clostridium histolyticum" J.Bacteriol.181. 923-933 (1998)
[Publications] Terawaki,Y.: "The complete nucleotide sequence of φCTX,a cytotoxin-converting phage of Pseudomonas aeruginosa : implications for phage evolution and horizontal gene transfer via bacteriophages" Mol.Microbiol.31. 399-419 (1999)
[Publications] Tsuda,M.: "Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme,hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase" Microbiology. 145. 495-501 (1999)
[Publications] Tsuda,M.: "Characterization of the MexC-MexD-OprJ multidrug efflux system in △ mexA-mexB-oprM mutants of Pseudomonas aeruginosa.Antimicrob" Agents Chemother.42. 1938-1943 (1998)
[Publications] Tsuda,M.: "Functional replacement of OprJ by OprM in the MexCD-OprJ multidrug efflux system of Pseudomonas aeruginosa" FEMS Microbiol.Lett.165. 21-27 (1998)
[Publications] Yoshimura,F.: "Capsular polysaccharide-fimbrial conjugate vaccine protects against Porphyromonas gingivalis infection in SCID mice reconstituted with human peripheral blood lymphocytes." Infect.Immun.66. 391-393 (1998)
[Publications] Yoshimura,F.: "Isolation and characteriza-tion of transposon-induced mutants of Porphyromonas gingivalis deficient in fimbriation" Microbial Pathogen.24. 25-23 (1998)
[Publications] Yoshimura,F.: "Active efflux norfloxacin by Bacteroides fragilis." Antimicrob.Agents Chem.42. 2119-2121 (1998)
[Publications] Yoshimura,F.: "Arg-gingipain acts as a major processing enzyme for various cell surface proteins in Porphyromonas gingivalis" J.Biol.Chem.273. 29072-29076 (1998)

1998 Fiscal Year Annual Research Report

細菌の病原性の分子遺伝学的研究

Principal Investigator

中澤 晶子 山口大学, 医学部, 教授 (40053053)

Research Products

[Publications] Danbara,H.: "TTG as the Initiation codon of Salmonella slyA,a Gene required for Survival within Macrophages" Microbiology and Immunology. (in press). (1999)

[Publications] Hayashi,H.: "The hsp operon is repressed by the hrc37 of the hsp70 operon in Staphylococcus aureus" Microbiol.Immunol.43. 19-27 (1999)

[Publications] Hayashi,H.: "Chromosome-determined zinc responsible operon czr in Staphylococcus aureus" Microbiol.Immunol.43. 115-125 (1999)

[Publications] Honda,T.: "Physical and genetic map of the genome of Vibrio parahaemolyticus : presence of two chromosomes in Vibrio species." Mol.Microbiol.(in press). (1999)

[Publications] Honda,T.: "Disappearance of glyceraldehyde 3-phosphate dehydrogenase from erythrocyte membrane by hemolysis with thermostable direct hemolysin of vibro parahaemolyticus or V.cholerae El Tor hemolysin." Microbiol.Immunol.(in press). (1999)

[Publications] Honda,T.: "Complete nucleotide sequences of 93-kb and 3.3kb plasmids of an enterohemorrhagic Escherichia coli O157 : H7 derived from Sakai outbreak" DNA Research. 5. 1-9 (1998)

[Publications] Honda,T.: "Diagnosis of bacterial enteric infections in children in Zambia" Acta Paediatrica Japonica. 40. 259-263 (1998)

[Publications] Honda,T.: "The effectiveness of hand-disinfection by a flow water system using electrolytic products of sodium chloride,compared with a conventional method using alcoholic solution in an intensive care unit" Critical Care. 2. 79-80 (1998)

[Publications] Honda,T.: "Nicking sites in A subunit of cholera toxin and Escherichia coli heat-labile enterotoxin for Vibrio cholerae hemagglutinin/protease." Toxicon. 36. 1001-1005 (1998)

[Publications] Honda,T.: "Close proximity of the tdh,trh and ure genes on the chromosome of Vibrio parahaemolyticus." Microbiology. 144. 2517-2523 (1998)

[Publications] Honda,T.: "Profiles of circulating inflammatory-and anti-inflammatory cytokines in patients with hemolytic uremic syndrome due to E.coli O157 infection." Cytokine. 10. 544-548 (1998)

[Publications] Ike,Y.: "First report of the isolation of high-level vancomycin-resistant Enterococcus faecium from a patient in Japan" Antimicrob.Agents Chemother. 42. 2150 (1998)

[Publications] Danbara,H.: "TTG as the Initiation codon of Salmonella slyA,s Gene required for Survival within Macrophages" Microbiology and Immunology. (in press). (1999)

[Publications] Hayashi,H.: "The hsp operon is repressed by the hrc37 of the hsp70 operon in Staphylococcus aureus" Microbiol.Immunol.43. 19-27 (1999)

[Publications] Hayashi,H.: "Chromosome-determined zinc responsible operon czr in Staphylococcus aureus" Microbiol.Immunol.43. 115-125 (1999)

[Publications] Honda,T.: "Physical and genetic map of the genome of Vibrio parahaemolyticus : presence of two chromosomes in Vibrio species." Mol.Microbiol.(in press). (1999)

[Publications] Honda,T.: "Disappearance of glyceraldehyde 3-phosphate dehydrogenase from erythrocyte membrane by hemolysis with thermostable direct hemolysin of Vibrio parahaemolyticus or V.cholerae El T or hemolysin." Microbiol.Immunol.(in press). (1999)

[Publications] Honda,T.: "Complete nucleotide sequences of 93-kb and 3.3kb plasmids of an enterohemorrhagic Escherichia coli O157 : H7 derived from Sakai outbreak" DNA Research. 5. 1-9 (1998)

[Publications] Honda,T.: "Diagnosis of bacterial enteric infections in children in Zambia" Acta Paediatrica Japonica. 40. 259-263 (1998)

[Publications] Honda,T.: "The effectiveness of hand-disinfection by a flow water system using electrolytic products of sodium chloride,compared with a conventional method using alcoholic solution in an intensive care unit" Critical Care. 2. 79-80 (1998)

[Publications] Honda,T.: "Nicking sites in A subunit of cholera toxin and Escherichia coli heat-labile enterotoxin for Vibrio cholerae hemagglutinin/protease." Toxicon. 36. 1001-1005 (1998)

[Publications] Honda,T.: "Close proximity of the tdh,trh and ure genes on the chromosome of Vibrio parahaemolyticus." Microbiology. 144. 2517-2523 (1998)

[Publications] Honda,T.: "Profiles of circulating inflammatory-and anti-inflammatory cytokines in patients with hemolytic uremic syndrome due to E.coli O157 infection." Cytokine. 10. 544-548 (1998)

[Publications] Ike,Y: "First report of the isolation of high-level vancomycin-resistant Enterococcus faecium from a patient in Japan" Antimicrob.Agents Chemother. 42. 2150 (1998)

[Publications] Ike,Y.: "Evidence of nosocomial Infection in Japan caused by high-level gentamicin-resistant Enterococcus faecalis and identification of the pheromone-responsive conjugative plasmid encoding gentamicin resistance" J.Clin.Microbiol.36. 2460-2464 (1998)

[Publications] Ike,Y.: "Efficient transfer of the pheromone-independent Enterococcus faecium plasmid pMG1 (Gm′)(65.1 Kirobases)to Enterococcus strains during broth mating" J.Bacteriol.180. 4886-4892 (1998)

[Publications] Kamio,Y.: "Complete nucleotide sequence and molecular characterization of the temperate stapylococcal bacteriopahge phi-PVL carrying Panton-Valentine leukocidin genes" Gene. 215. 57-67 (1998)

[Publications] Kamio,Y.: "An N-terminal region of LukF of Staphylococcal leukocidin/gamma-hemolysin crucial for the biologica activity of the toxin" Biosci.Biotechnol.Biochem.62(7). 1465-1467 (1998)

[Publications] Kamio,Y.: "Further study on the two pivotal parts of Hlg2 for the full hemolytic activity of staphylococcal gamma-hemolysin" Biosci.Biotechnol.Biochem.62(9). 1745-1750 (1998)

[Publications] Kamio,Y.: "Phosphorylation of LukS by protein kinase A is crucial for the LukS-specific function of the staphylococcal leukocidin on human polymorphonuclear leukocytes" Biosci Biotechnol.Biochem.62(9). 1834-1838 (1998)

[Publications] Kamio,Y.: "Crystal structure of staphylococcal LukF delineates conformational changes accompaning formation of a transmembrane channel" Nature(Structural Biology). 6(2). 6-12 (1998)

[Publications] Nakazawa,T.: "Persistent infection by Helicobacter pylori Down-regulates virus-specific CD8+ cytotoxic T cell response and prolongs viral infection" J Infect Dis. 177. 72-80 (1998)

[Publications] Nakazawa,T.: "Urease plays an important role in the chemotactic motility of Helicobacter pylori in a viscous environment" Infect.Immun.66. 4832-4837 (1998)

[Publications] Nakazawa,T.: "Nucleotide sequence and characterization of cdrA,a cell division-related gene of Helicobacter pylori" J.Bacteriol.180. 5263-5268 (1998)

[Publications] Nakazawa,T.: "Chemotaxis of Helicobacter pylori : a urease-independent response" J.Gastroenterol.33(S). 1-5 (1998)

[Publications] Oguma,K.: "botR/A is a positive regulator of botulinum neurotoxin and associated non-toxin protein genes in Clostridium botulinum A" Mol.Microbiol.29. 1009-1018 (1998)

[Publications] Oguma,K.: "Molecular composition of the 16S toxin produced by a Clostridium botulinum type D strain,1873" Microbiol.Immunol.42. 599-605 (1998)

[Publications] Oguma,K.: "Classification of Clostridium botulinum based on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and pulsed-field gel electrophoresis" Anaerobe. 4. (1998)

[Publications] Ohnishi,Y.: "Prostective effects of a culture supernatant of Lactobacillus acidophilus and antioxidants on ileal ulcer formation in rats treated with a nonsteroideal antiinflamatory drug" Microbiol.Immunol.42. 745-753 (1998)

[Publications] Ohnishi,Y.: "Hemolytic uremic syndrome in a patient witth Bacteroides fragilis infection" J.Jap.Assoc.Inf.Dis.72. 162-166 (1998)

[Publications] Okabe,A.: "Gene duplication and multiplicity of collagenases in Clostridium histolyticum" J.Bacteriol.181. 923-933 (1998)

[Publications] Terawaki,Y.: "The complete nucleotide sequence of φCTX,a cytotoxin-converting phage of Pseudomonas aeruginosa : implications for phage evolution and horizontal gene transfer via bacteriophages" Mol.Microbiol.31. 399-419 (1999)

[Publications] Tsuda,M.: "Cloning and characterization of the thiD/J gene of Escherichia coli encoding a thiamin-synthesizing bifunctional enzyme,hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase" Microbiology. 145. 495-501 (1999)

[Publications] Tsuda,M.: "Characterization of the MexC-MexD-OprJ multidrug efflux system in △ mexA-mexB-oprM mutants of Pseudomonas aeruginosa.Antimicrob" Agents Chemother.42. 1938-1943 (1998)

[Publications] Tsuda,M.: "Functional replacement of OprJ by OprM in the MexCD-OprJ multidrug efflux system of Pseudomonas aeruginosa" FEMS Microbiol.Lett.165. 21-27 (1998)

[Publications] Yoshimura,F.: "Capsular polysaccharide-fimbrial conjugate vaccine protects against Porphyromonas gingivalis infection in SCID mice reconstituted with human peripheral blood lymphocytes." Infect.Immun.66. 391-393 (1998)

[Publications] Yoshimura,F.: "Isolation and characteriza-tion of transposon-induced mutants of Porphyromonas gingivalis deficient in fimbriation" Microbial Pathogen.24. 25-23 (1998)

[Publications] Yoshimura,F.: "Active efflux norfloxacin by Bacteroides fragilis." Antimicrob.Agents Chem.42. 2119-2121 (1998)

[Publications] Yoshimura,F.: "Arg-gingipain acts as a major processing enzyme for various cell surface proteins in Porphyromonas gingivalis" J.Biol.Chem.273. 29072-29076 (1998)

中澤晶子山口大学, 医学部, 教授 (40053053)