1998 Fiscal Year Annual Research Report

動物発生における形成中心の分子的機能に関する比較発生学的総合研究

Research Project

Project/Area Number	08308039
Research Institution	TOKYO METROPOLITAN UNIVERSITY
Principal Investigator	八杉貞雄東京都立大学, 大学院・理学研究科, 教授 (70011591)
Co-Investigator(Kenkyū-buntansha)	武田洋幸名古屋大学, 理学部, 助教授 (80179647) 西田宏記東京工業大学, 生命理工学部, 助教授 (60192689) 浅島誠東京大学, 大学院・総合文化研究科, 教授 (00090564) 塩川光一郎東京大学, 大学院・理学系研究科, 教授 (20037295) 白井浩子岡山大学, 理学部, 助教授 (50090478)
Keywords	形成中心 / オーガナイザー / 比較発生学 / 分子発生学 / 胚葉形成 / 形態形成 / 脊索動物 / 棘皮動物
Research Abstract	本研究は後口動物の発生においてその体制の構築に中心的役割を果たす形成中心の機能を分子的および進化的側面から解明することを目的としている。本年度は研究の最終年度に当たるため、各研究分担者の研究を収束させ全体としてまとめるために、合宿形式の班会議を開催した。本年度に得られた主要な成果は以下の通りである。(1)ヒトデ卵の原腸形成因子を受け継いだD細胞の細胞表面に誘導物質が存在することを確認した。(2)ホヤ神経索の形成と誘導の関係を明らかにした。(3)ウニ外腸胚形成ペプチド結合蛋白質の機能解析が進んだ。(4)ウニの単離された中割球のLi処理による形成中心の誘導と魚類の卵黄細胞の誘導活性が明瞭に示された。(5)アクチビンリセプターとフォリスタチン遺伝子のプロモーターに結合する転写因子が単離された。(6)マウス原腸胚に発現し、正常胚と突然変異体で発現に差を生じる遺伝子の構造・機能解析が進んだ。(7)以上の研究に基づいて、無脊椎動物から脊椎動物への進化の過程で形成中心の分子カスケードと機能がどのように変化したかを推察することが可能になった。

Research Products

(20 results)

All Other

All Publications (20 results)

[Publications] Fukuda,K.: "CFKBP/SMAP;A novel molecule involved in the regulation of smooth muscle differentiation" Development. 125. 3535-3542 (1998)
[Publications] Ishii,Y.: "Region-specific expression of chicken Sox2 in the developing gut and lung epithelium:Regulation by epithelial-mesenchymal interactions" Dev. Dynamics. 213. 464-475 (1998)
[Publications] Saito,M: "Induction of metamorphosis in the sand dollar peronelia japonica by thyrcid hormones" Dev.Growth & Differ.40. 307-312 (1998)
[Publications] Hibino,T.: "CAB(Centrosome-attracting body):A novel structure closely related to unequal cleavages in the ascidian embryo" Dev.Growth & Differ.40. 85-95 (1998)
[Publications] Kumano,G.: "Maternal and zygotic expression of the endoderm-specific alkaline phosphatase gene in embryos of the ascidian Halocynthia roretzi" Dev. Biol.198. 245-252 (1998)
[Publications] Bates,W.R.: "Developmental roles of nuclear complex factors released during oocyle maturation in the ascidians Halocynthia roretzi and Bortenia villosa" Zool.Sci.15. 69-76 (1998)
[Publications] Kobayashi,M.: "overexpression of the forebrain-specific homeobox gene six3 induces rostral forebrain enlargement in zebrafish" Development. 125. 2973-2982 (1998)
[Publications] Yamanaka,T.: "A novel homeobox gene,dharma,can induce the organaizer in a non-cell autonomous manner" Genes & Dev.12. 2345-2353 (1998)
[Publications] Makita,R: "Zebrafish wnt 11 : pattern and regulation of the expression by the yolk cell and No tail activity" Mech. Dev.71. 165-176
[Publications] Yokota,C: "Pattern of gene expression in the core of Spemann's organizer and activin-treated ectoderm in Cynopus pyrrhogaster" Dev. Growth & Differ. 40. 335-341 (1998)
[Publications] Uochi,T: "XCIP(Xenopus homolog of cold-inducible RNA binding protein) is expressed transiently in developing pronephros and neural tissue" Gene. 211. 245-250 (1998)
[Publications] Shibata,M.: "Overexpression of S-adenosylmethionine docarboxylase (SAMDC) in early Xenopus embryos induces cell dissociation and inhibits transition from the blastula to gostrula" Int. J. Dev. Biol.42. 675-686 (1998)
[Publications] Abe,K.: "A systematic molecular genetics approach to study mammalian germ line development" Int. J. Dev. Biol.42. 1051-1065 (1998)
[Publications] Kawakami,S.: "Tctex 3, related to Drosophila polycomblike,is expressed in male germ cells and mapped to the mouse t-complex" Mammalian Genome. 9. 874-880 (1998)
[Publications] Vemet,C.: "Genetic mapping of 10 macrosatellites in the t complex region of mouse chromosome 17" Mammalian Genome. 9. 412 (1998)
[Publications] Mitsumoto,K.: "Differential expression of fos and jun family members in the developing chicken gastrointestinal tract" Oncogene. 16. 1611-1616 (1998)
[Publications] Narita,T.: "Sonic hedgehog expression in developing chicken digestive organs is regulated by epithelia-mesencluymal interactions" Dev.Growth & Differ.40. 67-74 (1998)
[Publications] 西田宏記: "ホヤの生物学" 東京大学出版会, (1998)
[Publications] 浅島誠: "発生のしくみが見えてきた" 岩波書店, 149 (1998)
[Publications] 駒崎伸二: "分子生物学イラストレイテッド" 羊土社, 334 (1998)

1998 Fiscal Year Annual Research Report

動物発生における形成中心の分子的機能に関する比較発生学的総合研究

Principal Investigator

八杉 貞雄 東京都立大学, 大学院・理学研究科, 教授 (70011591)

Research Products

[Publications] Fukuda,K.: "CFKBP/SMAP;A novel molecule involved in the regulation of smooth muscle differentiation" Development. 125. 3535-3542 (1998)

[Publications] Ishii,Y.: "Region-specific expression of chicken Sox2 in the developing gut and lung epithelium:Regulation by epithelial-mesenchymal interactions" Dev. Dynamics. 213. 464-475 (1998)

[Publications] Saito,M: "Induction of metamorphosis in the sand dollar peronelia japonica by thyrcid hormones" Dev.Growth & Differ.40. 307-312 (1998)

[Publications] Hibino,T.: "CAB(Centrosome-attracting body):A novel structure closely related to unequal cleavages in the ascidian embryo" Dev.Growth & Differ.40. 85-95 (1998)

[Publications] Kumano,G.: "Maternal and zygotic expression of the endoderm-specific alkaline phosphatase gene in embryos of the ascidian Halocynthia roretzi" Dev. Biol.198. 245-252 (1998)

[Publications] Bates,W.R.: "Developmental roles of nuclear complex factors released during oocyle maturation in the ascidians Halocynthia roretzi and Bortenia villosa" Zool.Sci.15. 69-76 (1998)

[Publications] Kobayashi,M.: "overexpression of the forebrain-specific homeobox gene six3 induces rostral forebrain enlargement in zebrafish" Development. 125. 2973-2982 (1998)

[Publications] Yamanaka,T.: "A novel homeobox gene,dharma,can induce the organaizer in a non-cell autonomous manner" Genes & Dev.12. 2345-2353 (1998)

[Publications] Makita,R: "Zebrafish wnt 11 : pattern and regulation of the expression by the yolk cell and No tail activity" Mech. Dev.71. 165-176

[Publications] Yokota,C: "Pattern of gene expression in the core of Spemann's organizer and activin-treated ectoderm in Cynopus pyrrhogaster" Dev. Growth & Differ. 40. 335-341 (1998)

[Publications] Uochi,T: "XCIP(Xenopus homolog of cold-inducible RNA binding protein) is expressed transiently in developing pronephros and neural tissue" Gene. 211. 245-250 (1998)

[Publications] Shibata,M.: "Overexpression of S-adenosylmethionine docarboxylase (SAMDC) in early Xenopus embryos induces cell dissociation and inhibits transition from the blastula to gostrula" Int. J. Dev. Biol.42. 675-686 (1998)

[Publications] Abe,K.: "A systematic molecular genetics approach to study mammalian germ line development" Int. J. Dev. Biol.42. 1051-1065 (1998)

[Publications] Kawakami,S.: "Tctex 3, related to Drosophila polycomblike,is expressed in male germ cells and mapped to the mouse t-complex" Mammalian Genome. 9. 874-880 (1998)

[Publications] Vemet,C.: "Genetic mapping of 10 macrosatellites in the t complex region of mouse chromosome 17" Mammalian Genome. 9. 412 (1998)

[Publications] Mitsumoto,K.: "Differential expression of fos and jun family members in the developing chicken gastrointestinal tract" Oncogene. 16. 1611-1616 (1998)

[Publications] Narita,T.: "Sonic hedgehog expression in developing chicken digestive organs is regulated by epithelia-mesencluymal interactions" Dev.Growth & Differ.40. 67-74 (1998)

[Publications] 西田宏記: "ホヤの生物学" 東京大学出版会, (1998)

[Publications] 浅島誠: "発生のしくみが見えてきた" 岩波書店, 149 (1998)

[Publications] 駒崎伸二: "分子生物学イラストレイテッド" 羊土社, 334 (1998)

八杉貞雄東京都立大学, 大学院・理学研究科, 教授 (70011591)