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1998 Fiscal Year Final Research Report Summary

Comparative embryological study on the molecular function of organizing centers in animal development

Research Project

Project/Area Number 08308039
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionTOKYO METROPOLITAN UNIVERSITY

Principal Investigator

YASUGI Sadao  Graduate School of Science, Tokyo Metropolita University, Professor, 大学院・理学研究科, 教授 (70011591)

Co-Investigator(Kenkyū-buntansha) TAKEDA Hiroyuki  Fac.Sci., Nagoya Univ., Associate Prof., 理学部, 助教授 (80179647)
NISHIDA Hiroki  Fac.Life Sci., Tokyo Inst.Technol., Associate Prof., 生命理工学部, 助教授 (60192689)
ASASHIMA Makoto  Graduate Sch.Sci., Tokyo Univ., Prof, 大学院・総合文化研究科, 教授 (00090564)
SHIOKAWA Koichiro  Graduate Sch.Sci., Tokyo UNiv., Prof., 大学院・理学系研究科, 教授 (20037295)
SHIRAI Hiroko  Fac.Sci., Okayama Univ., Associate Prof., 理学部, 助教授 (50090478)
Project Period (FY) 1996 – 1998
KeywordsOrganizing center / organizer / comparative embryology / molecular embryology / germ layer formation / morphogenesis / chordates / echinoderms
Research Abstract

The aim of this research group is to analyze from the view point of comparative embryology the molecular mechanims of the oraganizing center in deuterostome animals which plays important roles in determination of body axis and construction of body organization. Organizing center of each animal group has specific molecular action but, by the evolutionary constraints, ratains functionally and morphologically homologous domains. the main results obtained during 3 years can be summarized as follows :
1. The method to identify determinants in starfish was established. It will contribute much to the understanding of the molecular nature of organizing center in the echinoderms. Also genetic analysis of binding protein to exogastrulation- inducing peptide and of genes expressed spcifically in the micromeres which work as the organizing center was carried out.
2. As to the ascidinas, almost all aspects of mesoderm differentiation were elucidated. With using the antibody which recognizes the diffe … More rentiated state of the mesenchymal cells, the autonomy and determinants involved in the mesenchymal differentiation were analyzed. The analysis led to the conclusion that the differentiation of mesenchyme was reglutated both by determinants and cell-to-cell interactions. This study clearly demonstrated that there exists organizing center also in the ascidian embryos which develop with mosaic pattern.
3. The notion of organizing center in the fish and amphibians has been rather different so far. However, the study in this research group, especially on fish, showed that many genes are working in common between these two groups of vertebrates. Espcially, the organizing center of zebrafish was located to yolk syncythial cells, by elaborated experimental manipulations.
4. In the birds and mammals, the organizing center is the node. Cells differentiate into layers after going through this part of embryos. This process was precisely analyzed in the bird, while the formation and function of notochord in mammal was studied with T/t complex mutants. These studies made it possible to compare the organizing center of the higher vertebrates and that of other animal groups. Less

  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] H.Nishida: "Vegetal egg cytoplasm promotes gastrulation and is responsible for specification of vegetal Hastomeres in embryos of the ascidian Halognthia roretzi" Development. 122. 1271-1279 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Sakuma: "The protein tyrosine kinases of sea archin Anthecidaris crassipina" Zoological Science. 14. 307-312 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ariizumi,T.: "Activin treated urodele ectoderm:amodel experimental system of cardiogenesis" Int.J.Develop.Biol.40. 715-718 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokota,C.: "Pattern of gene expression in the core of Spemann's organizer and activin-treated ectoderm in Cyropus pyrrhogaster" Develop.Growth & Differ. 40. 335-341 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uochi,T.: "XCIRP(Xenopus homolag of cold-inducible RNA binding Protein)is expressed transiently in developing pronephros and neural tissue" Gene. 211. 245-250 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mizuno,T.: "Mesoderm induction in zebrafish" Nature. 383. 131-132 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nikaido,M.: "In vivo analysis using variants of zebrafish BMPR-IA:range of action and involvement of BMP in ectoderm patterning" Development. 126. 181-190 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,M.: "Specific arreit of spermatogenesis caused by apopotic cell death in transgenic mice" Genes to Cells. 1. 1077-1086 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Abe,K.: "Endoderm-specific gene expression in embryonic stem cells differentiated to embryoide bodies" Exptl.Cell.Res.229. 27-34 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Abe,K.: "A systematic molecular genetic approach to atudy mammalian germ line development" Int.J.Dev.Biol.42. 1051-1065 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shinga,J.: "Maternal and zygotic expression of mRNA for S-adenosyl methionine decarboxylase and its relevance to the unique polyamine composition in xenopus oocyte and enbryos" Biochim,Biophys.Acta. 1308. 31-40 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Urase,K.: "Analysis of mesenchymal influence on the pepsinogen gene expression in the epitheliun of chicken embryonic digestive tract" Roux's Arch.Dev.Biol.205. 382-390 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii,Y.: "Eonly specification of infestinal epitheliun in the chicken embryo:A study on the localization and regulation of Cdx A expression" Dev.Growth & Differ.39. 643-653 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Narita T.: "Sonic hedgeliog expression in the developing chicken digeatire orgams is regulated by epithelial-mesenchymal interacitons" Dev.Growth & Differ. 40. 67-74 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsmoto,K.: "Differential expression of the fos and jun family members in the developing gastrointestinal tract" Oncogene. 16. 1611-1616 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukuda,K.: "CFKBP/SMAP; a novel molecule involved in the regulation of smooth muscle differentiation" Development. 125. 3535-3542 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakamoto,N.: "Analysis of temporal expression pattern and cis-regulatory sequences of chicken pepsinogen A and C" Biochem.Biophys.Res.Commun.250. 420-424 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii,Y.: "Region-specific expression of chicken sox2 in the developing gut and lung epithelium;regulation by epithelial-mesenchymal interactions" Dev.Dyn.213. 464-475 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Nishida: "Vegetal egg cytoplasm promotes gastrulation and is responsible for specification of vegetal blastomers in embryos of the ascidian Halocynthia roretzi" Development. 122. 1271-1279 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Sakuma, H.Onodera, T.Suyemitsu and K.Yamasu: "The protein tyrosine kinases of the sea urchin Anthocidaris crassispina" Zool.Sci.14. 307-312 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ariizumi, T.Komazaki, S., Asashima, M., and G.M.Malacinski.: "Activin treated urodele ectoderm : a model experimental system for cardiogenesis." Int.J.Dev.Biol.40. 715-718 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokota, C., Ariizumi, T.and Asashima, M.: "Pattern of gene expression in the core of Spemann's organizer and activin-treated ectoderm in Cynops pyrrhegaster." Dev.Growth Differ.40. 335-341 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uochi, T.and Asashima, M.: "XCIRP (Xenopus homolog of cold-inducible RNA binding protein) is expressed transiently in developing pronephros and neural tissue." Gene. 211. 245-250 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mizuno, T., Yamaha, E., Wakahara, M., Kuroiwa, A.& Takeda, H.: "Mesoderm induction in zebrafish." Nature. 383. 131-132 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nikaido, M., Tada, M., Takeda, H., Kuroiwa, A.& Ueno, N.: "in vivo analysis using variants of zebrafish BMPR-IA : range of action and involvement of BMP inectoderm patterning." Development. 126. 181-190 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, M., Abe, K., Yoshinaga, K., Obinata, M., Furusawa, M.and abe, K.: "Specific arrest of spermatogenesis caused by apoptotic cell death in transgenic mice." Genes to Cells. 1. 1077-1086 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Abe, K., Niwa, H., Iwase, K., Takiguchi, M., Mori, M., Abe, S.-l., Abe, K.and Yamamura, K.: "Endoderm-specific gene expression in embryonic stem cells differentiated to embryoid bodies." Exptl. Cell Res.229. 27-34 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Abe, K., Ko, M.S.H.and MacGregor, G.R.: "A Systematic Molecular Genetic Approach to Study Mammalian Germ Line Development." Int.J.Dev.Biol.42. 1051-1065 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shinga, J., Kashiwagi, K., Tashiro, K., Igarashi, K., Shiokawa, K.: "Maternal and zygotic expression of mRNA for S-adenosylmethionine decarboxylase and its relevance to the unique polyamine composition in Xenopus oocytes and embryos" Biochim. Biophy. Acta.1308. 31-40 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Urase, K.Fukuda, Y., Ishii, N.Sakamoto and S.Yasugi: "Analysis of mesenchymal influence on the pepsinogen gene expression in the epithelium of chicken embryonic digestive tract. Roux's Arch." Dev.Biol.205. 382-390 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Ishii, K.Fukuda, H.Saiga, S.Matsushita and S.Yasugi: "Early specification of intestinal epithelium in the chicken embryo : A study on the localization and regultion of CdxA expression" Dev.Growth and Differ.39. 643-653 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Narita, Y.Ishii, T.Nohno, S.Noji and S.Yasugi: "Sonic hedgehog expression in the developing chicken digestive organs is regulated by epithelial-mesenchymal interactions." Dev.Growth and Differ. 40. 67-74 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Matsumoto, K.Saitoh, C.Koike, T.Narita, S.Yasugi H.and Iba: "Differential expression of the fos and jun family members in the developing chicken gastrointestinal tract." Oncogene. 16. 1611-1616 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Fukuda, Y.Tanigawa, G.Fujii, S.Yasugi and S.Hirohashi: "cFKBP/SMAP ; a novel molecule involved in the regulation of smooth muscle differentiation." Development. 125. 3535-3542 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Sakamoto, H.Saiga and S.Yasugi: "Analysis of temporal expression pattern and cisregulatory sequences of chicken pepsinogen a and C.Biochem. Biopys" Res. Commun.250. 420-424 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Ishii, M.Rex, P.J.Scotting and S.Yasugi, S.: "Region-spcific expression of chicken Sox2 in the developing gut and lung epithelium : regulation by epithelial-mesenchymal interactions" Dev.Dyn.213. 464-475 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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