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1998 Fiscal Year Final Research Report Summary

Studies on structure-function relationship of P450-multi-gene family from yeast and regulatory mechanism of their expression

Research Project

Project/Area Number 08406009
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionThe University of Tokyo

Principal Investigator

TAKAGI Masamichi  The University of Tokyo. Department of Biotechnology. Professor, 大学院・農学生命科学研究科, 教授 (50018339)

Project Period (FY) 1996 – 1998
KeywordsYeast / Candida maltosa / cytochrome P450 / CYP52 subfamily / substrate specificity / phylogenetic tree / n-alkane / fatty acid / hydroxylation
Research Abstract

Some yeast species can assimilate n-alkane as a sole source of carbon and energy. We have been using Candida maltosa (an industrial microbe for dicarboxylic acid production) and Yarrowia lipolytica (a haploid-type yeast) to investigate the metabolism of n-alkane, regulation of gene expression and the mechanism of membrane proliferation in the cells when they are grown on n-alkane. We had already found that cytochromes P450, which are the primary oxigenases for n-alkane metabolism, are inducibly synthesized together with the proliferation of intracellular membrane where cytochromes P450 locate in the presence of n-alkane and that there are more than two genes for P450 in the genome of Candida maltosa. In the present investigation, we have analyzed the mechanisms of regulation of gene expression and of membrane proliferation in Candida maltosa and the gene structures for cytochromes P450 and the regulation of gene expression in Yarrowia lipolytica to understand the response of these cells at the molecular level to the most hydrophobic substrate, n-alkane. The results of our experiments consist of the following 6 chapters.
1. Isolation and characterization of several cytochromes P450 genes, and the substrate specificity of the encoded enzymes in Candida maltosa.
2. Analysis of the cis-elements involved in the regulation of expression of cytochromes P450 genes in Candida maltosa.
3. The system we have constructed to isolate genes for the trans-factors required for cytochrome P450 gene expression in Candida maltosa.
4. The mechanisms of intracellular membrane proliferation induced by n-alkane in Candida maltosa.
5. Isolation and characterization of several cytochromes P450 genes in Yarrowia lipolytica.
6. Some related experiments.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] T.Zimmer et al.: "Relation between evolutionary distance and enzymatic properties among the members of the CYP52A sybfamily of Candida maltosa"Biochem.Biophys.Res.Commun. 251. 244-247 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Zimmer et al.: "Mutual conversion of fatty acid substrate specificity by a single amino acid exchange at position 527 in P450 Cm2 and P450Alk3 A"Europ.J.Biochem. 256. 398-403 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Iida et al.: "Cloning and characterization of an n-alkane-inducible cytochrome P450 gene essential for n-denane assimilation by Yarrowia lipolytica"Yeast. 14. 1387-1397 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Takewaka et al.: "Null mutation in IRE1 gene inhibits overproduction of microsomal cytochrome P450Alk1(CYP52A3)and proliteration of the endoplasmic reticulum in S.cerevisiae"J.Biochem(Tokyo). (in press). (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T. Zimmer, T. Iida, W.-H. Schunk, Y. Yoshida, and M. Takagi: "Relation between evolutionary distance and enzymatic properties among the member of the CYP52A subfamily of Candida maltosa."Biochem. Biophys. Res. Commun.. 251. 244-247 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Zimmer, U. Scheller, M. Takagi, and W.-H. Schunk: "Mutual conversion of fatty acid substrate specificity by a single amino acid exchange at position 527 in P450Cm2 and P450Alk3A."Europ. J. Biochem. 256. 398-403 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Iida, A. Ohta, and M. Takagi: "Cloning and characterization of an n-alkane-inducible cytochrome P450 gene essential for n-decane assimilation by Yarrowia lipolytica."Yeast.. 14. 1387-1397 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Takewaka, A. Ohta, and M. Takagi: "Null mutation in IREI gene inhibits overproduction of microsomal cytochrome P450Alk1 (CYP52A3) and proliferation of the endoplasmic reticulum in Saccharomyces cerevisiae."J. Biochem. (Tokyo). (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2001-10-23  

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