1996 Fiscal Year Annual Research Report

細胞内物質輸送を担う新しい分子モーター群KIFsの分子細胞生物学的研究

Research Project

Project/Area Number	08407001
Research Institution	The University of Tokyo
Principal Investigator	廣川信隆東京大学, 医学部, 教授 (20010085)
Co-Investigator(Kenkyū-buntansha)	岡田康志東京大学, 医学部, 助手 (50272430) 竹田扇東京大学, 医学部, 助手 (20272429) 原田彰宏東京大学, 医学部, 講師 (40251441) 金井克光東京大学, 医学部, 助教授 (80214427) 中田隆夫東京大学, 医学部, 助教授 (50218004)
Keywords	物理輸送 / キネシンスーパーファミリー / KIFs / 微小管 / 細胞
Research Abstract	1)新しいモーター分子群KIFsの同定とその分子細胞生物学的解析。 a)分子生物学的アプローチによりKIF1C,KIF6,7,8,9,10,11,12,13A,13B,14,15,16A,16B,17の15の新しいKIF遺伝子を発見しそのクローニングと組織別発現を解析した(Nakagawa et al.in preparation)。 b)KIF3A/3Bヘテロダイマーに結合する関連蛋白KAP3(Kinesin superfamily associated protein 3)を同定しその遺伝子のクローニング、一次構造の決定を行いさらにKAP3を単独あるいはKIF3A/3Bと同時に発現させその構造を電子顕微鏡で観察、KAP3がKIF3A/3Bヘテロダイマーの尾部に結合し球状の蛋白であることを明らかにした(Yamazaki et al.PNAS 1996)。 c)細胞骨格蛋白の遅い輸送の分子機構を明らかにするためcaged fluorescein tubulinの培養神経細胞内微量注入その後紫外線による蛍光活性化を行い抗蛍光抗体免疫細胞化学で解析した。又ニューロフィラメントM蛋白遺伝子にmycの標識をつけ生きたマウスの後根知覚神経細胞にアデノウィルスを用いて導入しその運命を免疫細胞化学的に解析しチュブリン、ニューロフィラメント蛋白ともポリマーとしてではなく小さなオリゴマーとして微小管にそって輸送されていることが分った。

Research Products
(11 results)

All Other

All Publications (11 results)

[Publications] Takemura,R.,et al.: "mRNA expression of KIF1A,KIF1B,KIF2,KIF3A,KIF3B,KIF4,KIF5 and cytoplasmic dynein during axonal regeneration." Journal of Neuroscience. 16. 31-35 (1996)
[Publications] Hirokawa,N.et al.: "Selective stabilization of tau in axons and MAP2C in cell bodies and dendrites contributes to polarized localization of cytoskeletal proteins in mature neurons." Journal of Cell Biology. 132. 667-679 (1996)
[Publications] Hirokawa,N.: "Organelle transport along microtubules-the role of KIFs(Kinesin superfamily proteins)." Trends in Cell Biology. 6. 135-141 (1996)
[Publications] Beech,P.L.et al.: "Localization of kinesin superfamily proteins to the connecting cilium of fish photoreceptors." Journal of Cell Science. 109. 889-897 (1996)
[Publications] Funakoshi,T.et al.: "Active transport of photoactivated tubulin molecules in growing axons revealed by a new electron microscopic analysis." Journal of Cell Biology. 133. 1347-1353 (1996)
[Publications] Terada,S.et al.: "Visualization of slow axonal transport in vivo." Science.273. 784-788 (1996)
[Publications] Yamazaki,H.et al.: "Cloning and characterization of KAP3:a novel kinesin superfamily associated protein of KIF3A/3B." Proceedings of the National Academy of Science of the USA. 93. 8443-8448 (1996)
[Publications] Grayer Wolf,S.et al.: "Interpreting a medium-resolution model of tubulin:comparison of Zinc-sheet and microtubule structure." Journal of Molecular Biology. 262. 485-501 (1996)
[Publications] Sato-Harada,R.et al.: "Microtubule-associated proteins regulate microtubule function as the track for intracellular membrane organelle transports." Cell Structure and Function. 21. 283-295 (1996)
[Publications] Hirokawa,N.: "The molecular mechanisms of organelle transport along microtubules:the identification and characterization of KIFs." Cell Structure and Function. 21. 357-367 (1996)
[Publications] Saito,N.et al.: "KIFC2,a novel C-terminal motor domaln type kinesin superfamily,is a neuron specific motor for the transport of multivesicular body-like organelles in dendrites." Neuron. (in press). (1997)

1996 Fiscal Year Annual Research Report

細胞内物質輸送を担う新しい分子モーター群KIFsの分子細胞生物学的研究

Principal Investigator

廣川 信隆 東京大学, 医学部, 教授 (20010085)

Research Products

[Publications] Takemura,R.,et al.: "mRNA expression of KIF1A,KIF1B,KIF2,KIF3A,KIF3B,KIF4,KIF5 and cytoplasmic dynein during axonal regeneration." Journal of Neuroscience. 16. 31-35 (1996)

[Publications] Hirokawa,N.et al.: "Selective stabilization of tau in axons and MAP2C in cell bodies and dendrites contributes to polarized localization of cytoskeletal proteins in mature neurons." Journal of Cell Biology. 132. 667-679 (1996)

[Publications] Hirokawa,N.: "Organelle transport along microtubules-the role of KIFs(Kinesin superfamily proteins)." Trends in Cell Biology. 6. 135-141 (1996)

[Publications] Beech,P.L.et al.: "Localization of kinesin superfamily proteins to the connecting cilium of fish photoreceptors." Journal of Cell Science. 109. 889-897 (1996)

[Publications] Funakoshi,T.et al.: "Active transport of photoactivated tubulin molecules in growing axons revealed by a new electron microscopic analysis." Journal of Cell Biology. 133. 1347-1353 (1996)

[Publications] Terada,S.et al.: "Visualization of slow axonal transport in vivo." Science.273. 784-788 (1996)

[Publications] Yamazaki,H.et al.: "Cloning and characterization of KAP3:a novel kinesin superfamily associated protein of KIF3A/3B." Proceedings of the National Academy of Science of the USA. 93. 8443-8448 (1996)

[Publications] Grayer Wolf,S.et al.: "Interpreting a medium-resolution model of tubulin:comparison of Zinc-sheet and microtubule structure." Journal of Molecular Biology. 262. 485-501 (1996)

[Publications] Sato-Harada,R.et al.: "Microtubule-associated proteins regulate microtubule function as the track for intracellular membrane organelle transports." Cell Structure and Function. 21. 283-295 (1996)

[Publications] Hirokawa,N.: "The molecular mechanisms of organelle transport along microtubules:the identification and characterization of KIFs." Cell Structure and Function. 21. 357-367 (1996)

[Publications] Saito,N.et al.: "KIFC2,a novel C-terminal motor domaln type kinesin superfamily,is a neuron specific motor for the transport of multivesicular body-like organelles in dendrites." Neuron. (in press). (1997)

廣川信隆東京大学, 医学部, 教授 (20010085)