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1998 Fiscal Year Final Research Report Summary

Elucidation of the mechanisms of the impairment of functional neuron networks and establishment of the therapeutic strategies.

Research Project

Project/Area Number 08407007
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionKagawa Medical University

Principal Investigator

NAGAO Seigo  Kagawa Medical University, Faculty of Medicine, Professor, 医学部, 教授 (60100947)

Co-Investigator(Kenkyū-buntansha) TOKUDA Masaaki  Kagawa Medical University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (10163974)
KOBAYASHI Ryoji  Kagawa Medical University, Faculty of Medicine, Professor, 医学部, 教授 (00020917)
HIRASHIMA Mitsuomi  Kagawa Medical University, Faculty of Medicine, Professor, 医学部, 教授 (70109700)
SAKAMOTO Haruhiko  Kagawa Medical University, Faculty of Medicine, Professor, 医学部, 教授 (60106549)
TAKEUCHI Yoshiki  Kagawa Medical University, Faculty of Medicine, Professor, 医学部, 教授 (20116619)
Project Period (FY) 1996 – 1998
Keywordscalcium / Cdk5 / p35 / neuronal cell / calbrain / kindlling / ischemic neuronal death / ecalectin / calmodulin-dependent enzyme
Research Abstract

1. Anti-allergic drugs such as amlexanox and cromolyn bound to S-100, visin-like protein, and 14-3-3, and inhibited their functions.
2. During the developmental processes of rat brain, the expression level of cyclin-dependent kinase 5 (Cdk5) did not alter, and its activaot, p35, did.
3. Calmodulin-dependent protein kinases (CaMK) I and IV were involved in the formation of long-term potentiation using rat hippocampal slices. A novel calcium-binding protein, calbrain, inhibited CaMK II.
4. Using a calcineurin-specific inhibitor, FK506, calcineurin was shown to be involved in the epileptogenesis of the kindled rats.
5. By the study using SAM (Senescence-Accelerated Mouse), the impairment of blood-brain barrier was shown to occur during the aging processes.
6. The hypothermic treatment worked effectively to protect the delayed neuronal death after the brain ischemia. It was important to raise the body temperature to the normal level as slow as possible after this therapy.
7. Neuronal transplantation of fetal hippocampal cells induced the recovery of the memory and learning impaired rats.
8. Transcription and translation of growth hormone was enhanced by the inhibition of calcineurin using immunosuppressive drugs (FK506 and cyclosporin A).

  • Research Products

    (44 results)

All Other

All Publications (44 results)

  • [Publications] T.Masaki: "Enhanced expression of the protein kinase substrate annexin I in human hepatocellular carcinoma" Hepatology. 24・1. 72-81 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Tokuda: "Direct evidence of the production of IgA by tonsillar lymphocytes and the binding of IgA to the nephropathy patients" Acta Otolaryngologica. Supple.523. 182-184 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Yan: "Cloning and functional expression of cDNAs encoding human and rat pancreatic polypeptide receptors." Proceedings of the National Academic of Sciences of the USA. 93. 4661-4665 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] B.Y.Ahmed: "FA chronological study of the expression of glial fibrillary acidic protein and calbindin-D28K by reactive astrocytes in the electrically lesioned rat brain." Neuroscience Research. 26・3. 271-278 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Moriwaki: "Immunosuppressant FK506 prevents mossy fiber sprouting induced by kindling stimulation." Neuroscience Research. 25. 191-194 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Morita: "Fos induction in rat brain neurons following stimulation of the hepatoportal Na-sensitive mechanism." American Journal of Physiology. 272. R913-R923 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Ueno: "Age related changes in the brain transfer of blood-borne horseradish peroxidase in the hippocampus of senescence-accelerated mouse." Acta Neuropatholgy. 93. 233-240 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Ueno: "Age-related changes in barrier function in mouse brain II. Accumulation of serum albumin in the olfactory bulb of SAM mice increased with aging" Archives of Gerontology and Geriatrics. 25. 321-331 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Nagao: "Treatment of acute in tracranial hypertension with RU51599, a selective kappa opioidagonist." Acta Neurochir.Suppl.70. 198-201 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] I Bemana: "OPC-21268, an orally effective, nonpeptide arginine vasopressin Vl receptor antagonist reduces vasogenic brain edema." Acta Neurochir. Suppl.70. 194-197 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Seki: "Induction of eosinophil chemotactic factor production from human peripheral blood mononuclear cells by solubilized BALL-1, a B cell-lymphoma line." Int.Arch.Allergy Immunology. 114・S-1. 2-5 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Masada: "Embryonic transplantation and ischemic memory deficit" Neuroscience Research. 27. 249-255 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Tokuda: "In volvement of calmodulin-dependent protein kinases I and IV in long-term potentiation." Brain Research. 755. 162-166 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Oyama: "Two distinct anti-allergic drugs, amlexanox and cromolyn, bind to the same kinds of calcium binding proeins, except calmodulin, in bovine lung extract." Biochemical and Biophysical Ressearch Communications. 240. 341-347 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Niimi: "Effect of continuous intravenous injection of interleukin-6 and pretreatment with cyclooxygenase inhibitor on brain c-fos expression in the rat" Neuroendocrinology. 66・10. 47-53 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Nakamura: "The effect of post-ishemic hypothermia on neuronal survival after l-month in gerbils" Advances in Neurotrauma Research. 9. 6-9 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.Matsumoto: "Human ecalectin, a variant of human galectin-9, is a novel eosinophil chemoattractant produced by Tlymphocytes" J.Bri.Chem.(in.press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.Yokote: "Molecular cloning and gene expression of growth hormone-releasing peptide receptor in rat tissues." Peptides. 19・1. 15-20 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Yamagami: "Suppression of hyperemia after brain ischemia by L-threo-3,4-dihydroxyphenylserine." NeuroReport. 9. 2939-2943 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Q.Xie: "Evaluation of pulmonary afferent fibers in the nucleus tractus solitarius : a horseradish peroxidase and c-fos like immunohistochemical study in the rat" J.Auton.Nerv.Sys.74. 120-125 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Yamaguchi: "The reversible change of GluR2 RNA editing in gerbil hippocampus in course of ischemic tolerance." J.Cerebral Blood Flow and Metabolism. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Yamaguchi: "Calbrain, a novel two EF-hand calcium-binding protein that suppresses Ca^<2+>/calmodulin-dependent protein kinase II activity in the brain." J.Biol.Chem.274・6. 3610-3616 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Masaki et al.: "Enhanced expression of the protein kinase substrate annexin I in human hepatocellular carcinoma." Hepatology. 24 (1). 72-81 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Tokuda et al.: "Direct evidence of the production of IgA by tonsillar lymphocytes and the binding of IgA to the nephropathy patients." Acta Otolaryngologica. Supple.523. 182-184 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Yan.: "Cloning and functional expression of cDNAs encoding human and rat pancreatic polypeptide receptors." Proceedings of the National Academic of Sciences of the U.S.A.93. 4661-4665 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] B.Y.Ahmed et al.: "A chronological study of the expression of glial fibrillary acidic protein and calbindin-D28K by reactive astrocytes in the electrically lesioned rat brain." Neuroscience Research. 26 (3). 271-278 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Moriwaki et al.: "Immunosuppressant FK506 prevents mossy fiber sprouting induced by kindling stimulation." Neuroscience Research. 25. 191-194 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Morita et al.: "Fos induction in rat brain neurons following stimulation of the hepatoportal Na-sensitive mechanism." American Journal of Physiology. 272. R913-R923 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Ueno et al.: "Age related changes in the brain transfer of blood-borne horseradish peroxidase in the hippocampus of senescence-accelerated mouse." Acta Neuropathol. 93. 233-240 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Ueno et al.: "Age-related changes in barrier function in mouse brain II.Accumulation ofserum albumin in the olfactory bulb of SAM mice increased with aging." Archives of Gerontology and Geriatrics. 25. 321-331 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Nagao et al.: "Treatment of acute intracranial hypertension with RU51599, a selective kappa opioidagonist." Acta Neurochir. (Suppl.), 70. 198-201 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] I.Bemana et al.: "OPC-21268, an orally effective, nonpeptide arginine vasopressin Vl receptorantagonist reduces vasogenic brain edema." Acta Neurochir. (Suppl.), 70. 194-197 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Seki et al.: "Induction of eosinophil chemotactic facto production from human peripheral blood mononuclear cells by solubilized BALL-1, a B cell lymphoma line." Int.Arch.Allergy Immunol. 114 (S-1). 2-5 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Masada et al.: "Embryonic transplantation and ischemic memory deficit." Neuroscience Res.27. 249-255 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Tokuda et al.: "Involvement of calmodulin-dependent protein kinases I and IV in long-term potentiation." Brain Res. 755. 162-166 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Oyama et al.: "Two distinct anti-allergic drugs, amlexanox and cromolyn, bind to the same kinds of calcium binding proeins, except calmodulin, in bovine lung extract" Biochem.Biophys.Res.Commun. 240. 341-347 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Niimi et al.: "Effect of continuous intravenous injection of interleukin-6 and pretreatment with cyclooxygenase inhibitor on brain c-fos expression in the rat." Neuroendocrinology. 66 (10). 47-53 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Nakamura et al.: "The effect of post-ishemic hypothermia on neuronal survival after 1-month in gerbils." Advances in NeurotraumaResearch.9. 6-9 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R.Matsumoto et al.: "Human ecalectin, a variant of human galectin-9, is a novel eosinophil chemoattractant produced by T lymphocytes." J.Br.Chem。. (in pres). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R.Yokote et al.: "Molecular cloning and gene expression of growth hormone-releasing peptide receptor in rat tissues." Peptides. 19 (1). 15-20 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Yamagami et al.: "uppression of hyperemia after brain ischemia by L-threo-3,4-dihydroxyphenylserine" NeuroReport. 9. 2939-2943 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Q.Xie et al.: "Evaluation of pulmonary afferent fibers in the nucleus tractus solitarius : a horseradish peroxidase and c-fos like immunohistochemical study in the rat." J.Auton, Nerv. System. 74. 120-125 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamaguchi et al.: "The reversible change of GluR2 RNA editing in gerbil hippocampus in course of ischemic tolerance." J.Cerebral Blood Flow and Metabolism. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamaguchi, et al.: "Calbrain, a novel two EF-hand calcium-binding protein that suppresses Ca^<2+>/calmodulin-dependent protein kinase II activity in the brain" Journal of Biological Chemistry. 274 (6). 3610-3616 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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