1998 Fiscal Year Final Research Report Summary
The regulation of cell differentiation and morphogenesis on mamalian early development
| Project/Area Number |
08408033
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Osaka University |
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Principal Investigator |
HAMADA Hiroshi Institute for Molecular and Cellular Biology, Osaka University, 細胞生体工学センター, 教授 (00208589)
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| Co-Investigator(Kenkyū-buntansha) |
SAIJHO Yukio Institute for Molecular and Cellular Biology, Osaka University, 細胞生体工学センター, 助手 (20260333)
FUJII Hideta Institute for Molecular and Cellular Biology, Osaka University, 細胞生体工学センター, 助手 (10273723)
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| Project Period (FY) |
1996 – 1998
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| Keywords | Left-right asymmetry / Lefty / transcriptional regulation |
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| Research Abstract |
Both lefty-1 and lefty-2 genes are expressed on the left side of developing mouse embryos and are implicated in left-right (L-R) axis formation. With the use of transgenic analysis, the transcriptional regulatory regions of these genes responsible for their L-R asymmetric expression have now been investigated. The 9.5-kb upstream region of lefty-1 and the 5.5-kb upstream region of lefty-2 reproduced the expression pattern of the corresponding gene. Examination of deletion constructs revealed the presence of a left side-specific enhancer (ASE) that is essential and sufficient for lefty-2 asymmetric expression. In contrast, the asymmetric expression of lefty-1 was shown to be determined by a combination of bilateral enhancers and a right side-specific silencer (RSS). The 9.5-kb region of lefty-1 and the 5.5-kb region of lefty-2 responded to iv and inv, upstream genes of lefty-1 and lefty-2. The regulation of lefty-2 by iv and inv was mediated by ASE. These results suggest that, in spite of the similarities between lefty-1 and lefty-2, different regulatory mechanisms underlie their asymmetric expression.
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[Publications] Mitsuya, K., Meguro, M., Sui, H., Schulz, T.C., Kugoh, H., Hamada H., and Oshimura, M: "Epigeneric reprogramming of the human H19 gene in mouse embryonic cells does not erase the primary parental imprinting"Genes Cells. 3. 245-255 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Meno, C., Shimono, A., Saijoh, Y., Yashiro, K., Mochida, K., Ohishi, S., Noji, S., Kondoh, H. and Hamada, H: "lefty-1 is required for left-right determination as a regulator of lefty-2 and nodal"Cell. 94. 287-297 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Yoshioka, H., Meno C., Koshiba, K., Sugihara, M., Itoh, H., Ishimaru, Y., Inoue, T., Ohuchi, H., Semina, E.V., Murray, J.C., Hamada, H., and Noji, S.: "Ptx2, a bicoid homeobox gene, is involved in a Lefty-signaling pathway in determination of left-right asymmetry"Cell. 94. 299-305 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Mochizuki, T., Saijoh, Y.,Tsuchiya, K., Shirayoshi, Y., Takai. S., Taya, C., Yonekawa, H., Yamada, K., Nihei, H., Nakatsuji, N., Overbeek, P., Hamada,_H., and Yokoyama, T.: "Cloning of inv, a gene that controls left/right asymmetry and kidney development"Nature. 395. 177-181 (1998)
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「研究成果報告書概要(欧文)」より
