• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1998 Fiscal Year Final Research Report Summary

Characterization of fatty acid conversion enzymes of soybean and their application for improvement of soybean oil quality

Research Project

Project/Area Number 08456005
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionSaga University

Principal Investigator

TAKAGI Yutaka  Saga Univ., Fac.Agriculture, Professor, 農学部, 教授 (30039341)

Co-Investigator(Kenkyū-buntansha) TSUKAMOTO Takuji  Saga Univ., Fac.Agriculture, Professor, 農学部, 教授 (70136597)
Project Period (FY) 1996 – 1998
Keywordssoybean / fatty acid / desaturation enzymes
Research Abstract

Previously, we have reported to establish many fatty acid-mutants from X-ray irradiated soybean progenies.
In this research, we analyzed their mutants using genetical and molecular biological approach, and then identified the gene family that is involved in fatty acid synthesis pathway. These results lead to conclusion that sop] and sop2 genes control the high palmitic acid character, st1 and st2 genes control the high stearic acid character, ol gene controls high oleic acid character, and fan and fanx genes control low linolenic acid character, respectively.
We could isolate some cDNA clones encoding stearoyl-ACP desaturase, microsomal omega-6 fatty acid desaturase and microsomal omega-3 fatty acid desaturase from soybean seed cDNA library. Three of them were identified as corresponding clones to stl, ol and fan genes, respectively.
Furthermore, we analyzed the expression of these genes under phytohormone treatments, and found that mRNA accumulations of both plastidal and microsomal omega-6 desaturase genes coordinately decreased, in contrast, that of omega-3 desaturase genes coordinately increased.

  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Y.Takagi and S.M.Rahman: "Inheritance of high oleic acid content in the seed oil of soybean M23" Theor.Appl.Genet.92. 179-182 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.M.Rahman,Y.Takagi and T.Kumamaru: "Low linolenate sources at the Fan locus in soybean lines M-5 and IL-8" Breed.Sci.46. 155-158 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.M.Rahman,Y.Takagi and T.Kinoshita: "Genetic analysis of palmitic acid contents using two soybeanmutants,J3 and J10" Breed.Sci.46. 343-347 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.M.Rahman,Y.Takagi and T.Kinoshita: "Genetic control of high oleic acid content in the seed oil of two soybeanmutants" Crop Sci.36. 1225-1128 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.M.Rahman,Y.Takagi: "Inheritance of reduced linolenic acid content in soybean seed oil" Theor.Appl.Genet.299-302 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.M.Rahman,Y.Takagi and T.Kinoshita: "Genetic control of high stearic acid content in the seed oil of two soybeanmutants" Theor.Appl.Genet.95. 772-776 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.K.Wasala,T.Kinoshita S.Arima and Y.Takagi: "Agronomic performances of low linolenic acid soybean mutant lines developed from cultivar Bay" Bull.Fac.Agr.,Saga Univ.82. 29-36 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.M.Rahman,T.Kinoshita,T.Anai,S.Arima and Y.Takagi: "Genetic relationships of soybean mutants for different linolenic acid contents" Crop Sci.38. (702-706)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kinoshita,S.M.Rahman,T.Anai and Y.Takagi: "Inter-locus relationship between genes controlling palmitic acid contents in soybean" Breed.Sci.48. 377-381 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Yamashita,T.Kinoshita,S.M.Rahman,K.Okada,T.Anai and Y.Takagi: "Genetic analysis of restriction fragment length polymorphism on fatty acid synthesis on soybean mutants and their progenies:I.Low linolenic acid mutants with a microsomal ω-3 fatty acid desaturase cDNA as a probe" Bull.Fac.Agr.,Saga Univ.83. 31-36 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Kinoshita,S.M.Rahman,T.Anai and Y.Takagi: "Genetic analysis of restriction fragment length polymorphism on fatty acid synthesis on soybean mutants and their progenies : II.High oleic acid mutants with a microsomal ω-6 fatty acid desaturase cDNAs as probe" Bull.Fac.Agr.,Saga Univ.83. 37-42 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.M.Rahman,T.Kinoshita,T.Anai and Y.Takagi: "Genetic analysis of restriction fragment length polymorphism on fatty acid synthesis on soybean mutants and their progenies:III.High stearic acid mutants with a stearoyl-acyl carrier protein desaturase cDNA as a probe" Bull.Fac.Agr.,Saga Univ.83. 43-48 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Takagi,S.M.Rahman,T.Anai,S.K.Wasala T.Kinoshita and M.Khalekuzzaman: "Development of a reduced linolenate soy mutant by re-irradiation and its genetic analysis" Breed.Sci.49. 1-6 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.M.Rahman,T.Kinoshita,T.Anai and Y.Takagi: "Genetic relationships between loci for palmitate contents in soybean mutants" J.Hered.(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Takagi and S.M.Rahman: "Inheritance of high oleic acid content in the seed oil of soybean M23" Theor.Appl.Genet.92. 179-182 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.M.Rahman, Y.Takagi and T.Kumamaru: "Low linolenate sources at the Fan locus in soybean lines M-5 and IL-8" Breed.Sci.46. 155-158 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.M.Rahman, Y.Takagi and T.Kinoshita: "Genetic analysis of palmitic acid contents using two soybeanmutants" J3 and J10. Breed.Sci.46. 343-347 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.M.Rahman, Y.Takagi and T.Kinoshita: "Genetic control of high oleic acid content in the seed oil of two soybean mutants" Crop Sci.36. 1125-1128 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.M.Rahman, Y.Takagi: "Inheritance of reduced linolenic acid content in soybean seed oil" Theor.Appl.Genet.94. 299-302 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.M.Rahman, Y.Takagi and T.Kinoshita: "Genetic control of high stearic acid content in the seed oil of two soybean mutants" Theor.Appl.Genet.95. 772-776 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.K.Wasala, T.Kinoshita, S.Arima, and Y.Takagi: "Agronomic performances of low linolenic acid soybean mutant lines develpoed from cultivar Bay" Bull.Fac.Agr.Saga Univ.82. 29-36 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.M.Rahman, T.Kinoshita, T.Anai, S.Arima and Y.Takagi: "Genetic relationships of soybean mutants for differet linolenic acid contents" Crop Sci.38. 702-706 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kinoshita, S.M.Rahman, T.Anai and Y.Takagi: "Inter-locus relationship between genes controlling palmitic acid contents in soybean" Breed.Sci.48. 377-381 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Yamashita, T.Kinoshita, S.M.Rahman, K.Okada, T.Anai and Y.Takagi: "Genetic analysis of restriction fragment length polymorphism on fatty acid synthesis on soybean mutants and their progenies : I.Low linolenic acid mutants with a microsomal omega-3 fatty acid desaturase cDNA as a probe" Bull.Fac.Agr., Saga Univ.83. 31-36 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Kinoshita, S.M.Rahman, T.Anai and Y.Takagi: "Genetic analysis of restriction fragment length polymorphism on fatty acid synthesis on soybean mutants and their progenies : II.High oleic acid mutants with two microsomal omega-6 fatty acid desaturase cDNAs as probes" Bull.Fac.Agr.Saga Univ.83. 37-42 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.M.Rahman, T.Kinoshita, T.Anai and Y.Takagi: "Genetic analysis of restriction fragment length polymorphism on fatty acid synthesis on soybean mutants and their progenies : III.High stearic acid mutants with a stearoyl-acyl carrier protein desaturase cDNA as a probe" Bull.Fac.Agr.Saga Univ.83. 43-48 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Takagi, S.M.Rahman, T.Anai, S.K.Wasala, T.Kinoshita and M.Khalekuzzaman: "Development of a reduced linolenate soy mutant by re-irradiation and its genetic analysis" Breed.Sci.49. 1-6 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.M.Rahman, T.Kinoshita, T.Anai and Y.Takagi: "Genetic relationships between loci for palmitate contents in soybean mutants" J.Hered. (in press). (1999)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-12-08  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi