1997 Fiscal Year Final Research Report Summary
Improvement of petal pigmentation and flowering, and regulation of senescence in cut flowers through introducing fructan synthesis genes
| Project/Area Number |
08456017
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
園芸・造園学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KAWABATA Saneyuki The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assistant Professor, 大学院・農学生命科学研究科, 助手 (10234113)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Anthocyanin / Fructan / Eustoma grandiflorum / Transgenic / Fructosyltransferase |
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| Research Abstract |
(1) We investigated the influence of low light intensity on petal pigmentation in lisianthus. Shading whole plants or the stem and leaf parts significantly reduced the intensity of petal color, whereas shading flowers had no effects on it. When detached flowers with peduncles were placed in sucrose solutions, their petal color became more intense as the sucrose concentration increased, concomitantly with increased levels of chalcone synthase, chalcone isomerase, and dihydroflavonol 4-reductase transcripts. These results suggest that reduced supply of photosynthates is responsible for pale petal color under low light intensity.
(2) Leaf section produced 8 to 18 shoots per culture on MS media containing 0.05 mg/l IBA, 1 mg/l BA, and 0.1 mg/l GA3. These shoots were placed on 1/2 strength MS media containing IBA after they were incubated on MS media containing IAA.They rooted within 40 to 50 days.
(3) We attempted to introduce 6-sucrose : fructan fructosyltransferase (6-SFT) gene from barley and SacB gene from Bacilus subtilis into lisianthus plants using pB121 and Agrobacterium tumefacience LBA4404. SacB gene was fused to vacuolar targeting sequence of sweet potato sporamin. The transformants were screened by kanamycin resistance and PCR analysis. We could not obtain any transformats for 6-SFT However, for SacB gene, 32 explants were kanamycin resistant and 100 % out of 15 kanamycin-resistant plants contained SacB gene according to PCR analysis.
(4) The rooting and subsequent growth of the transformants were poor, and they did not reach the flowering stage. Reexamination of culture condition after rooting and acclimation condition will be needed before evaluating the effect of introducing fructan synthesis genes on flower pigmentaion.
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