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1997 Fiscal Year Final Research Report Summary

Analysis of the molecular mechanisms of host range determination of nucleopolyhedovirus

Research Project

Project/Area Number 08456035
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 蚕糸・昆虫利用学
Research InstitutionNagoya University

Principal Investigator

KOBAYASHI Michihiro  Nagoya University, Fac.of Ag.Professor, 農学部, 教授 (60111837)

Co-Investigator(Kenkyū-buntansha) IKEDA Motoko  Nagoya University, Fac.of Agr.Assist.Prof., 農学部, 助手 (20262892)
Project Period (FY) 1996 – 1997
Keywordsnucleopolyhedrovirus / host range / viral DNA / cytopathic effect / budded virion / polyhedrin
Research Abstract

Autographa californica (Ac), Bombyx mori (Bm), Hyphantria cunea (Hc) and Spodoptera exigua (Se) nucleopolyhedroviruses (NPVs) were each inoculated to SF21cells, BmN-4 cells, SpIm cells and Se301 cells, and cytopathic effect (CPE) manifestation, viral DNA production, badded virion yield into the culture medium, viral structural protein production, polyhedrin (the matrix protein of polyhedra) production, and polyhedron formation in the inoculated cells were comparatively examined. The results showed that AcNPV were able to multiply and yield budded virions in all of four cell lines examined, but production of polyhedrin and polyhedra occurred only SF21cells, SpIm cells and Se301 cells, and not in BmN-4 cells.
BmNPV multiplied to a high titre in BmN-4 cells and to a low titre in SpIm cells in which only a few number of cells manifested CPE.No clear CPE was observed in SF21 and Se301 cells inoculated with BmNPV.HcNPV multiplied unequivocally and produced polyhedra in SpIm and Se301 cells. In SF21 cells, HcNPV yielded budded virions only to a low titre and produced neither polyhedrin nor polyhedra. In HcNPV-inoculated BmN-4 cells, significant amount of viral DNA accumulated, but accumulation of viral structural protein and polyhedrin and budded virion yield were never detected. SeNPV multiplied clearly only in Se301 cells, and appeared to induce apoptotic form of cell death in many cells of SpIm culture.
These results indicate that NPVs exhibit complex feature in the cells depending upon both the species of NPV and cultured cells being inoculated, and establish important basis for the analysis of molecular mechanisms of host range determination in NPVs.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Satoshiro, H., Ikeda, M and Kobayashi, M.: "Comparative analysis of Bombyx moru (Lepidsprera,Bombycidee) deusovirus type2 polypeptides synthesized in vivo and in vitro" Appl.Entomol.Zool. 31. 154-159 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoshiro, H., Ikeda, M.and Kobayashi, M.: "Identificetson of a novel virus-spectic in vitro frauslatoon prodicts in the midgut of the silkuoorm,Bombyx mori,imtected with B mori denst virus type2" J.Sericul.Sci.Jpn. 66. 38-47 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chaeychomsri, S., Ikeda, M.and Kobayashi, M.: "Temporal regulation of Bom byx mori (Lepicloptera : Bombycidee) nucleopolyhedro virus DNA polymerase geme expression" Appl.Entomol.Zool. 32. 418-421 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sato, K., Komata, M., Sato, T., Enei, H.et al.: "Baculovirus-mediated expression of a gene for trehalase of the mealxoorm,Teuebrio molitor,in insect cells SF-9,and larroe of tae..." Insect Biochem.Mol.Biol. 27. 1007-1016 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikeda, M.and Yan ashita, O: "Structure of the gene eucoding vitellin-degrading protease of the silkeoorm,Bonbyx mori" J.Sericult Sci.,Jpn. 66. 346-350 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sotoshiro, H., Ikeda, M., and Kobayashi, M.: "Comparative analysis of Bombyx mori (Lepidoptera : Bombycidae) densovirus type 2 polypeptides synthesized in vivo and in vitro." Appl.Entomol.Zool.31. 154-159 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sotoshiro, H., Ikeda, M., and Kobayashi, M.: "Identification of a novel virus-specific in vitro translation product in the midgut of the silkworm, Bombyx mori, infected with B.mori densovirus type 2." J.Sericult.Sci.Jpn. 66. 38-47 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chaeychomsri, S., Ikeda, M., and Kobayashi, M.: "Temporal regulation of Bombyx mori (Lepidoptera : Bombycidae) nucleopolyhedrovirus DNA polymerase gene expression." Appl.Entomol.Zool.32. 418-421 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, K., Komata, M., Sato, T., Enei, H., Kobayashi, M., and Yaginuma, T.: "Baculovirus-mediated expression of a gene for trehalase of the mealworm, Tenebrio molitor, in insect cells, SF-9, and larvae of the cabbage armyworm, Mamestra brassicae." Insect Biochem.Molec.Biol.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikeda, M., and Yamashita, O.: "Structure of the gene encoding vitellin-degrading protease of the silkworm, Bombyx mori." J.Sericult.Sci.Jpn. 66. 346-350 (1997)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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