1997 Fiscal Year Final Research Report Summary
Studies on Genetic Resources of the silkworm, Bombyx mori
| Project/Area Number |
08456037
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
蚕糸・昆虫利用学
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
FUJII Hiroshi KYUSHU UNIVERSITY,AGRICULTURE,PROFESSOR, 農学部, 教授 (10038268)
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| Co-Investigator(Kenkyū-buntansha) |
BANNO Yutakai KYUSHU UNIVERSITY,AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (50192711)
KAWAGUCHI Yutaka KYUSHU UNIVERSITY,AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (80038306)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Bombyx mori / nucleotide sequence / chymotrypsin inhibitor / rouge mutant / new non-molting mutant / kidney shaped egg / in situ hybridization / sol (soft and limp) gene |
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| Research Abstract |
New mutants, rouge and non-molting were found. The ro gene was located on the sex chromosome by linkage analysis. Nucleotide sequence of chymotrypsin inhibitor 13 (CI-13) was determined.
The adult having homozygous ki gene lays kidney-shaped eggs which can not develop complete embryo. These results suggest that maternal mRNAs of the ki egg differ from maternal mRNAs of the normal egg. Using the mRNAs extracted from unfertilized eggs of a normal and ki mutant, RT-PCR and differential display methods was performed to elucidate the difference between them. The results indicated that maternal mRNA from ki mutant are not different from that of a normal..
The sol gene for a recessive mutant named "soft and limp" was shown to be linked with E gene mapped to the end of the 6th linkage group. A three-point test involving the E^<ca> and the F gene located on the same linkage group indicated that the arrangement of three gene loci is in the order of sol-E^<ca>-F The locus of the sol gene was determined to be -21.1 cM of the 6th linkage group. In oeder to clarify this result, in situ hybridization with a RNA probe derived from the Bombyx mori Antennapedia gene was carried out, the fluorescence signal was detected at an internal region of a chromosome, not at its distal end.
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