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1997 Fiscal Year Final Research Report Summary

Molecular biological analysis on protein secretion of koji mold

Research Project

Project/Area Number 08456045
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionThe University of Tokyo

Principal Investigator

KITAMOTO Katsuhiko  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Professor, 大学院・農学生命科学研究科, 教授 (20272437)

Project Period (FY) 1996 – 1997
KeywordsKoji mold / protein secretion / molecular biology / protein disulfide isomerase / alpha-glucosidase / Aspergillus
Research Abstract

Koji mold (Aspergillus oryzae) is one of the most important fungi, which has been used for sake, soy sauce, soy bean paste. It has an ability of a large amount of several useful enzymes. This resesarch project was fucused on the production of useful protein.
1. A gene encoding protein disulfide isomerase, which Plays an important role in secreted protein foldind in ER,was cloned and the structure of the gene was analyzed.
2. In order to examine the mechanism of induction of amylase genes, a comparison among alpha-glucosidase gene (agdA), alpha-amylase gene (amyB), and glucoamylase gene(glaA), construction of deletion mutants of the promoter sequence were done. Region III was identified as an important region for amylase induction and novel strong promoter
with tandem rpeats of the region III was developed.
3. Actin related protein gene (arpA), which is involved in nuclear division, was cloned and determined its nucleotide sequence. The arpA disruptant showed defect in nucler division as well as an abnormal morphorogy of hyper-branched hyphae. This disruptant forming many hyphae tips secretes a large amount of alpha-amylase campared with its wlid type strain.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] B.R.Lee: "Cloning Characterization and Overexpression of a Gene(pdi A) Encoding Protein Disulfide Isomerase of Aspergillus oryzae" J.Ferment.Bioeng.82. 538-543 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Minetoki: "Deletion Analysis of Promoter Elementsof the Aspergillus oryzae agdA Gene Encoding α-glucosidase" Curr.Genet.30. 432-438 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 北本 勝ひこ: "麹菌(A.oryzae)の分子生物学的解析の最近の進展" 日本農芸化学会誌. 71. 27-27 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 峰時 俊貴: "清酒醸造における麹の働きと遺伝子解析" 日本農芸化学会誌. 71. 34-39 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] B.R.Lee, O.Yamada, K.Kitamoto, and K.Takahashi: "Cloning, characterization and overexpression of a gene (pdiA) encoding protein disulfide isomerase of Aspergillus oryzae" J.Ferment.Bioeng.82. 538-543 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Minetoki, Y.Nunokawa, K.Gomi, K.Kitamoto, C.Kumagai, and G.Tamura: "Deletion analysis of promoter elements of the Aspergillus oryzae agdA gene encoding alpha-glucosidase" Curr.Genet. 30. 432-438 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Kitamoto: "Recent progress on molecular biological analysis of koji mold (A.oryzae)" Nippon Nogeikagaku kaishi. 71. 27-28 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Minetoki, and K.Kitamoto: "Gene analysis and role of rice koji in sake brewing" Nippon Nogeikagaku kaishi. 71. 34-39 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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