1997 Fiscal Year Final Research Report Summary
Screening, functional analysis and application of polyunsaturated fatty acid-desaturase inhibitors
| Project/Area Number |
08456050
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SHIMIZU Sakayu KYOTO UNIVERSITY,Graduate School of Agriculture, Professor, 農学研究科, 教授 (70093250)
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| Co-Investigator(Kenkyū-buntansha) |
OGAWA Jun KYOTO UNIVERSITY,Graduate School of Agriculture, Instructor, 農学研究科, 助手 (70281102)
KATAOKA Michihiko KYOTO UNIVERSITY,Graduate School of Agriculture, Instructor, 農学研究科, 助手 (90252494)
KOBAYASHI Michihiko KYOTO UNIVERSITY,Graduate School of Agriculture, Lecturer, 農学研究科, 講師 (70221976)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Desaturase / Inhibitor / Propyl gallate / Curcumin / Ca channel blocker / polyunsaturated fatty acid / Furan fatty acid / Docosahexaenoic acid |
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| Research Abstract |
(A)Four groups of polyunsaturated fatty acid-desaturase inhibitors were found.
(1)Lignan compounds ; both (+) and (-) isomers are DELTA5-desaturase noncompetitive inhibitors. (+)-Sesamin and (-)-asarinin in sesame seeds specifically inhibit DELTA5-desaturase.
(2)Alkyl gallate : noncompetitive inhibitors of DELTA5 and DELTA6-desaturases. Propyl gallate inhibits DELTA5 and DELTA6-desaturases. Esterification of carboxylate and 3-hydroxy group in its molecule are essential for the inhibitory activity.
(3)Curcumin : noncompetitive inhibitors of DELTA5 and DELTA6-desaturases. The half part of its symmetrical structure is important for the inhibitory activity. Aromatic structure conjugated with double bond, 4-hydroxy and 3-methoxy group in its molecule is essential.
(4)Dihydropyridine Ca channel blocker : uncompetitive inhibitors of DELTA5 or DELTA6-desaturases. Nicardipine and nifedipine specifically inhibit DELTA5 and DELTA6-desaturases, respectively.
(B)The effects of antioxidants and related compounds, which affects on polyunsaturated fatty acid biosynthesis, on the furan fatty acid (F-acid) biosynthesis were investigated. p-Nitrobenzoic acid was found to inhibit F-acid biosynthesis.
(C)The effects of antioxidants and related compounds, which affects on polyunsaturated fatty acid biosynthesis, on docosahexanoic acid (DHA) biosynthesis were investigated. p-Toluic acid affected on DHA biosynthesis. It increased 22 : 5 and decreased 20 : 5.
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[Publications] Kawashima, H.Kamada, N., Sakuradani, E., Jareonkitmonkol, S., Akimoto, K.& Shimizu, S.: "Production of 8,11,14,17-cis-eicosatetraenoic acid a DELTA5 desaturase-defective mutants of an arachidonic acid-producing fungus, Mortierella alpina." J.Am.Oil Chem.Soc.74. 455-459 (1997)
Description
「研究成果報告書概要(欧文)」より
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