1997 Fiscal Year Final Research Report Summary
MORECULAR GENETICAL INVESTIGATION OF DOMESTIC ANIMALS AND POULTRY USING HIGHLY POLYMORPHIC MICROSATELLITE DNA
| Project/Area Number |
08456152
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
KUNIEDA Tetsuo OKAYAMA UNIVERSITY,FACULTY OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (80178011)
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| Co-Investigator(Kenkyū-buntansha) |
MORITOMO Yasuo KYUSYU TOKAI UNIVERSITY,FACULTY OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (70148972)
OIKAWA Takuro OKAYAMA UNIVERSITY,FACULTY OF AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (90160407)
SATO Katsunori OKAYAMA UNIVERSITY,FACULTY OF AGRICULTURE,PROFESSOR, 農学部, 教授 (10033325)
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| Project Period (FY) |
1996 – 1997
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| Keywords | MICROSATELLITE / DOMESTIC ANIMAL / POULTRY / QUAIL / CATTLE / CHONDRODYSPLASIA / MUTATION / MAPPING |
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| Research Abstract |
The aims of the present study are to construct genetic linkage maps of the Japanese quail, to identify gene responsible for chondrodysplasia of Japanese brown cattle, and to characterize genetic feature of the particular population of Japanese brown cattle.
To isolate microsatellite DNA of Japanese quail, microsatellite enriched genomic DNA library was screened with dinucliotide repeats. Consequently, we isolate clones containing CA dinucleotide repeats, but the dinucleotide repeats of most of these clone are interrupted by another sequences. These interrupted dinucleotide repeats are not likely to be polymorphic and thus could not be used as marker loci.
In a particular population of Japanese brown cattle, a chondrodysplastic dwarfism is inherited as an autosomal recessive trait. By using 2200 bovine microsatellite markers and a paternal half-sib pedigree including 48 affected and 28 normal animals, we investigated linkage between the locus responsible for the bovine chondrodysplasi and microsatellite loci. Consequently, we mapped the locus on a particular region of bovine chromosome 6. The region of the bovine chromosome 6 is homologous to regions of human and mouse choromosomes on which FGFR3 gene is localized. Since this gene is known to be responsible for human chondrodysplasia, the FGFR3 gene is a candidate gene for the bovine chondrodysplasia.
Genome wide scanning of the 5 cattle obtained from a particular population of Japanese brown cattle were performed by using microsatellite markers. About 50% of the microsatellite markers, which are polymorphic in the population, were homozygous condition in the cattle. The significant portion of these homozygous loci were revealed to localize on particular region of chromosomes. These findings suggest that the microsatellite loci could be a useful markers for investigating the genetic feature of populations of domestic animals.
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[Publications] Kunieda, T., Kumagai, T., Tsuji, T., Ozaki, T., Karaki, H., and Ikadai, H.: "A mutation in endothelin-B receptor gene causes myenteric aganglionosis and coat color spotting in rat." DNA Res.3. 101-105 (1996)
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[Publications] Karaki, H., Mitui-Saito, M., Takimoto M., Oda, K., Okada, T., Ozaki, T., and Kunieda, T.: "Lack of endothelin ETB receptor binding and function in the rat with a mutant ETB receptor gene." Biochem.Biophys.Res.Commun.222. 139-143 (1996)
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[Publications] Simonaro, C.M., Haskins, M.E., Kunieda, T., Evans, S.M., Visser, J.W., Schuchman, E.H.: "Bone marrow transplantation in newborn rats with mucopolysaccharidosis type VI : biochemical, pathological, and clinical findings." Transplantation. 63. 1386-1393 (1997)
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[Publications] Tsuji, T., Watanabe, Y., Katoh, H., Sato, K., Kunieda, T.: "Cloning and mapping of the mouse Gpx2 gene encoding gastrointestinal glutathione peroxidase." J.Vet Med, Sci.(in press.).
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