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1997 Fiscal Year Final Research Report Summary

Histological studies on differntiation and systematization of vitamin A-storing cells in vertebrates.

Research Project

Project/Area Number 08457002
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionTokyo Medical and Dental University

Principal Investigator

WAKE Kenjiro  Tokyo Medical and Dental University, School of Medicine, Professor, 医学部, 教授 (00046963)

Co-Investigator(Kenkyū-buntansha) FUJIEDA Hiroki  Tokyo Medical and Dental University, School of Medicine, Assistant, 医学部, 助手 (70280972)
EKATAKSIN Wichai  Tokyo Medical and Dental University, School of Medicine, Assistant, 医学部, 助手 (50223441)
SATO Tetsuji  Tokyo Medical and Dental University, School of Medicine, Associate Professor, 医学部, 助教授 (10162447)
Project Period (FY) 1996 – 1997
KeywordsVitamin A / Vitamin A-strong cell / Hepatic stellate cell / Collagen / Intralobular heterogeneity / kidney / Lamprey / Mesangial cell
Research Abstract

In order to know the heterogeneity of stored vitamin A in the hepatic stellate cells, we examined pig liver by the use of gold chloride method. Vitamin A-lipid droplet morphometry was performed under oil immersion and the calculated volumes were plotted on computerized maps. The hepatic lobule was sub divided into 5-zone/5-region comparmentalization. Distribution of values exhibited a district zonal gradient, higher at peripheral zones 1 and 2, dcreased over intermediate zone 3 toward centrilobular zones 4 and 5. Within a single zone, variations were obvious, forming a regional gradient ; higher at periportal than mideptal regions. Along the periphery, inlet venules of portal veins were quantified, revealing an occurrence rate of 60% at periportal, and 5% at midesptal regions.
Abundant vitamin A was found in the mesangial cells in the lamprey kidney. We discussed similarities of the this cell type with the hepatic stellate cell. From our both present and previous findings on the lamprey, we concluded that vitamin A is stored in various splanchnic organs but not in the somatic organs. In the mammalian species, vitamin A is stored only in the liver, however, after the administration of excess vitamin A the fibroblastic cells in the splanchnic organs incorporated and stored it in the cytopalsm. Vitamin A-storing cell system is a system of mesenchymal cell system existing only in the splanchnic organs of vertebrates and vitamin A-storing cells reveal heterogeneity not only among splanchnic organs but also within a single organ.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Bauer, P.: "Mesangial cells of lamprey,Lampetra japonica,store vitaminA" Arch HistoL CytoL. 59. 71-78 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 和気 健二郎: "器官-その新しい視点。肝臓:組織" 生体の科学. 47. 363-367 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 和気 健二郎: "肝臓の構造" Human Science. 7. 13-17 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 和気 健二郎: "肝類洞壁細胞研究の百年" 解剖誌. 72. 407-423 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawai, Y.: "Uptake of lithium carmine by sinusoidal endotheliaL and Kupfter cells of the ratliver,newinsights into the classicol vital staining and the RES." Cell Tissue Res. 292(2). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zou, Z.: "Zonal and regionaL differences identified from precision mapping of vitaminA-storinglipid droplets of the hepatic stellate cells in pigliver" Hepatology. (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wake, K.: "Functional Heterogeneity of Liver Tissue" Vidal-Vanoclocha,F,(ed,) Spinger;New York., 242 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ekataksin: "Progress in Liver Diseases" Saunders,Boy er JL,Ockner RK(eds), 1-30 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Bauer P,Wake K: "Mesangial cells of lamprey, Lampetra japonica, store vitamin A." Arch Histol Cytol. 59. 71-78 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wake K,Kawai Y: "Phagocytosis of collagen fibrils by vitamin A-storing cells in the lamprey, L.japonica." Acta Anat Nippon. 71. s402 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wake K: "Structure of the liver" Human Science. 7. 13-17 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wake K: "One hundred years of sinusoidal cells in the liver" Acta Anat Nippon. 72. 402-423 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Wake K: "Sinusoidal structure and dynamics In ; Vidal-Vanaclocha F.ed.Functional Hetero-geneity of Liver Tissue : From Cell Lineage diversity to Sublobular Component-specific Pathogenesis" Springer, New York. 57-67 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ekataksin W,Wake K: "New concepts in biliary and vascular anatomy of the liver. In : Boyer JL,Ockner RK.Eds Progress in Liver Disease Vol XV" Saunders, Philadelphia. 1-30 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawai Y,Wake K: "Uptake of lithium carmine by sinusoidal endothelial and Kupffer cells of the rat liver : new insights into the classical vital staining and the reticulo-endothelial system." Cell Tissue Res. 292 (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Zou Z,Ekataksin W,Wake K: "Zonal and regional differences identification from precision mapping of vitamin A-storing lipid droplets of the hepatic stellate cells in pig liver : a novel concept of addressing the intralobular areas of heterogeneity." Hepatology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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