1997 Fiscal Year Final Research Report Summary
Morphological and molecular cell biological study of apoptosis in mammalian morphogenesis
| Project/Area Number |
08457005
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SHIOTA Kohei Kyoto Univ.Graduate School of Medicine, Dept.of Anat.& Develop.Biol., Professor, 医学研究科, 教授 (80109529)
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| Co-Investigator(Kenkyū-buntansha) |
TAKIGAWA Toshiya Kyoto Univ.Graduate School of Medicine, Dept.of Anat.& Develop.Biol., Instructor, 医学研究科, 助手 (90263095)
ISHIBASHI Makoto Kyoto Univ.Graduate School of Medicine, Dept.of Anat.& Develop.Biol., Instructor, 医学研究科, 助手 (30232341)
MORI Chisato Kyoto Univ.Graduate School of Medicine, Dept.of Anat.& Develop.Biol., Associate, 医学研究科, 助教授 (90174375)
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| Project Period (FY) |
1996 – 1997
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| Keywords | apoptosis / programd cell death / organogenesis / developmental abnormality / myocarditis / testis / heat shock protein |
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| Research Abstract |
1. Apoptotic cell death in the developing limb of mouse fetuses was examined sequentially on days 11-15 of gestation by means of Nile blue sulfate staining. It was found that during digit formation, programd cell death (PCD) begins at the proximal portion of the interdigital mesenchyme and subsequently expands distally. Therefore, the initial PCD that occurs in the interdigital zones may determine the proximal ends of digital separation and also contribute to the demarcation between the palm (sole) and digits (toes). During digit separation, the areas of PCD in the interdigital zones were found to become larger and expand distally on day 13, which may be necessary for the separtion of digits and for determining the the interdigital area to disappear.
In addition, intense PCD was observed in the radila (tibial) and ulnar (fibular) margins of the hand and foot plates for 4-5 days. Such PCD at marginal areas seems to prevent the formation of supernumerary digits (pre-and poistaxial polydac
… More
tyly) and other digital malformations.
2. HSP105 (105 k-Da heat shock protein) was detected scarcelyt in mouse embryos at gestational day (GD) 8, but its level increased markedly from GD9 to 11 and then decreased gradually by GD14. HSP105 was found to be localized in condensed cells and condensed bodies showing the typical characteristics of apoptosis at the interdigital region of the limb.. These findings suggest that HSP105 plays important roles during mouse embryo development.
3. Mouse spermatogenic cell apoptosis was examined in mature male mice after administration of mitomycin C (MMC) using DNA nick end labeling, transmission electron microscopy (TEM) and electrophoresis. It was found tha in the testis of MMC-treated mice, (1) apoptotic cell treated with DNA feagmentation is induced by MMC dose-dependently, (2) apoptotic cell death was found to occur most commonly in spermatogonia and less frequently in spermatocytes, and (3) apoptotis cell death induced by MMC was not specific for the seminiferous stage of the tubules. It is suggested that spermatogenic cell apoptosis induced by MMC might be involved in its testicular toxicity. Less
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[Publications] Mori, C., Nakamura, N., Dix, D.J., Fujioka, M., Nakagawa, S., Shiota, K.and Eddy, E.M.: "Morphological analysis of germ cell apoptosis during postnatal testis development in normal and Hsp70-2 knockout mice." Develop.Dynamics. 208. 125-136 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Doi, R., Wada, M., Hosotani, R., Lee, J.-U., Koshiba, T., Fujimoto, K., Mori, C., Nakamura, N., Shiota, K.and Imamura, M.: "Role of apoptosis in duct obstruction-induced pacreatic involution in rats." Pancreas. 14. 39-46 (1997)
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[Publications] Li, Z.-L., Chisaka, O., Koseki, H., Akasaka, T., Ishibashi, M.and Shiota, K.: "Heat shock-induced homeotic transformations of the axial skeleton and associated shifts of Hox gene expression domains in mouse embryos." Reprod.Toxicol.11(6). 761-770 (1997)
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