• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1997 Fiscal Year Final Research Report Summary

Regulatory mechonisms for pxocytosis

Research Project

Project/Area Number 08457011
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

OHMORI Harunori  Kyoto-Univ.Faculty of Med.Dop.of Physiology Professor, 医学研究科, 教授 (30126015)

Project Period (FY) 1996 – 1997
KeywordsSynapse / Ca ion / GABA / MNTB / purkinje neuron
Research Abstract

(1) Potentiation of GABAergic postsynaptic currents in the Purkinje cells. Spontaneous inhibitory postsynaptic currents (IPSCs) and evoked IPSCs were recorded from cultured Purkinje cells of the rat. Size of these GABAergic IPSCs were increased after membrane depolarization of Purkinje cells. The increase was due to the increased GABA response in the postsynaptic membrane rather than the increased release of GABA presynapitcally, and was likely coupled with the activation of PLC through the increased intracellular Ca concentration and the IP3 induced Ca release.
(2) Postnatal development of phasic transmission in the clayx type synapse in the auditory relay nucleus of rat. Synaptic transmission in the medial nucleus of the trapezoid body (MNTB) of rats was analyzed in postnatal days of 4-13 (P4-P13) by applying the whole cell patch recording technique to brain slices. In P4-P6 animals, evoked EPSCs fluctuated extensively in amplitude and occurred in marked asynchrony, follwed by spontaneous EPSCs. With development of animals, the evoked EPSCs increased in amplitude, and the rise time became faster. In addition the synaptic transmission became phase-locked. The coefficient of variation (CV) of EPSC amplitude decreased with development (0.32(]SY.+-。[)0.03 for P4-P5 and 0.05(]SY.+-。[)0.01 for P9-P11). The amplitude of mEPSCs did not change throughout the postnatal days investigated (-30.2(]SY.+-。[)0.3 pA,at -70 mV). The CV was dependent on extracellular Ca2+ concentration ([Ca2+]o) and was reduced with the increase of [Ca2+]o, and this [Ca2+]o dependence was shifted towards lower [Ca2+]o with development. Direct patch recording of the presynaptic terminals demonstrated an increase in Ca2+ currents during these postnatal days. The phase-locked high-fidelity transmission in this synapse is achieved with development likely through the increase of Ca2+ currents and Ca2+ sensitivity of transmitter release mechanisms in the presynaptic terminal.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Ishii et al: "Hypotoric stiumulation induced Ca^<2+> release from IP_3 sensitive stores in a queen monkey kidney cell line." Journal of Physiology. 493.2. 371-384 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kataoka.Y.& Ohmori.H: "Of known neurotronsmittess,glutamate is the most lihaly to be released from chick cochlear nait cells" Journal of Neuro Physiology. 76(3). 1870-1880 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hashimoto.J.et al: "Release of Ca^<2+> is the crucial step for the potentiation of IPSCs in the cultured cerebellar Purkinje cells of rat." Journal of Physiology. 497.3. 611-627 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kataoka, Y.et al: "A postsynoptic excitatory amino acid from sporter with chloride conductance functionally regulated by neuronal activity in cerebellar Purkinje cells" Journal of Neuroscience. 17(18). 7017-7024 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chuhman.& Ohmori A.: "Yostnatal development of phare-locked high-fidlity synagtic transmission in the medial nueleus of the trapezdid body of the rat." Journal of Neuroscience. 18(1). 512-520 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishii, T., Hashimoto, T.& Ohmori, H.: "Hypotonic stimulation induced Ca2+ release from IP3 sensitive internal stores in a green monkey kidney cell line." Journal of Physiology. 493.2. 371-384 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kataoka, Y & Ohmori, H.: "Of known neurotransmitters, glutamate is the most likely to be released from chick cochlear hair cells." J.Neurophysiology. 76(3). 1870-1880 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hashimoto, T., Ishii, T.& Ohmori, H.: "Release of Ca2+is the crucial step for the potentiation of IPSCs in the cultured cerebellar Purkinje cells of rat." Journal of Physio logy. 497.3. 611-627 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kataoka, Y., Morii, H., Watanabe, Y.and Ohmori, H.: "A postsynaptic excitatory amino acid transporter with chloride conductance functionally regulated by neuronal activity in cerebellar Purkinje cells." Journal of Neurosciences. 17(18). 7017-7024 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chuhma, Nao & Ohmori, H.: "Postnatal development of phaselocked high fidelity synaptic transmission in the medial nucleus of the trapezoid body of the rat." Journal of Neuroscience. 18(1). 512-520 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1999-03-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi