1997 Fiscal Year Final Research Report Summary
Roles of proteases on cell-to-cell adhesion and deadhesion
| Project/Area Number |
08457013
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
SHIOSAKA Sadao NAIST,STRUCT.CELL BIOL., PROFESSOR, バイオサイエンス研究科, 教授 (90127233)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Nobuaki OSAKA MEDICAL CENTER FOR ATERNAL AND CHILD HEALTH,DIRECTOR, 部長 (10250341)
YOSHIDA Shigetaka NAIST,STRUCT.CELL BIOL., ASS.PROFESSOR, バイオサイエンス研究科, 助教授 (20230740)
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| Project Period (FY) |
1996 – 1997
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| Keywords | NEUROPSIN / MIGRATION / CELL-TO-CELL ADHESION / CELL-TO-CELL DEADHEASION / FIBRONECTIN / SERINE PROTEASE / NEURON / KERATINOCYTE |
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| Research Abstract |
Some proteases have been suggested to be related to cell dynamics in such processes as cell death, cell migration, cell-to-cell adhesion and deadhesion, and neural process elongation, pathfinding and axonal rearrangement, we postulated that (a) novel protease (s) might be released from neurons and relate to various cell dynamics described above.
Neuropsin was cloned from the mouse brain and was shown to be localized in mouse hippocampal pyramidal neurons and also epidermal keratinocytes. Recombinant neuropsin effectively cleaved fibronectin which is a major ECM protein expressed in variety of tissues. The specific cleavage by recombinant neuropsin as shown in the present study might directly affect fibronectin's functions as a cell adhesion molecule, because the N-terminal 5 FnI domains contain the main fibrin-binding site. Cell migration and cell atachment activity were analyzed with model system using CHO cell which expressing alpha5beta1 integlin. Recombinant neuropsin effectively decreased these activity when fibronectin was used as cell adhesion molecule.
Neuropsin knock-out mouse was made and histological, bichemical, physiological and behavioral analysis is now in progress.
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[Publications] Inoue, N., Kuwae, K., Ishida-Yamamoto, A., Iizuka, H., Shibata, M., Yoshida, S., Kato, K.and Shiosaka, S.: "Expression of neuropsin in the keratinizing epithelial tissue-immunohistochemical analysis of wild-type and nude mice." J.Investigative Dermatology. (in press.).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Momota, Y., Yoshida, M., Ito, J., Shibata, M., Kato, K., Sakurai, K., Matsumoto, K., and Shiosaka, S.: "Blockade of neuropsin, a serine protease, ameliorates kindling epilepsy." Eur.J.Neurosci.10. 760-764 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Shimizu, C., Yoshida, S., Shibata, M., Kato, K., Momota, Y., Matsumoto, K., Shiosaka, T., Midorikawa, R., Kamachi, T., Kawabe, A.and Shiosaka, S.: "Characterization of recombinant and brain neuropsin, a plasticity-related serine protease." J.Biol.Chem.(in press.).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Chen, Z-L., Momota, Y., Kato, K., Taniguchi, M., Inoue, N., Shiosaka, S., and Yoshida, S.: "Expression of neuropsin mRNA in the mouse embryo and the pregnant uterus." J.Histochem.Cytochem. (in press.).
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[Publications] Okabe, A., Momota, S., Yoshida, S., Hirata, A., Ito, J., Nishino, S., Shiosaka, S.: "Kindling induces neuropsin mRNA in the mouse brain" Brain Res.729. 116-120 (1996)
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[Publications] Suzuki, J., Yoshida, S., Chen, Z-L., Momota, Y., Kato, K., Hirata, A.and Sadao Shiosaka: "Ontogeny of neuropsin mRNA expression in the mouse brain." Neurosci.Res.23. 345-351 (1995)
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「研究成果報告書概要(欧文)」より
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[Publications] Chen, Z-L., Yoshida, S., Kato, K., Momota, Y., Suzuki, J., Tanaka, T., Ito, J., Nishino, H., Aimoto, S., Kiyama, H.and Shiosaka, S.: "Expression and activity-dependent changes of a novel limbic-serine protease gene in the hippocampus." J.Neurosci.15. 5088-5097 (1995)
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「研究成果報告書概要(欧文)」より
