1997 Fiscal Year Final Research Report Summary
Bilirubin as a biodefense factor against oxidative stress and its clinical application
| Project/Area Number |
08457045
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Tokyo medical and dental university, Medical Research Institute |
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Principal Investigator |
YAMAGUCHI Tokio Tokyo Med.& Dent.Univ., Medical.Res.Inst., Associate Prof., 難治疾患研究所・遺伝疾患(遺伝生化), 助教授 (30134745)
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| Co-Investigator(Kenkyū-buntansha) |
SUGIMOTO Akiko Tokyo Med.& Dent.Univ., Inst.Med.& Dent.Engineerig, Res.Assistant, 医用器材研究所, 助手 (60143608)
SAITO Fumiko Tokyo Med.& Dent.Univ., Medical.Res.Inst., Res.Assistant, 難治疾患研究所, 助手 (10158917)
HORIO Fumihiko Nagoya Univ., Dept.of Agriculture, Associate Prof., 農学部, 助教授 (20165591)
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| Project Period (FY) |
1996 – 1997
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| Keywords | oxidative stress / antioxidant / bilirubin |
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| Research Abstract |
We examined the possibility that bilirubin physiologically acts as an antioxidant by using survy-prone ODS-od/od rats treated woth endotoxin (lipopolysaccharide : LPS). Recently, billirubin oxidative metabolites were isolated from human urine, and named biotropyrrin-a and biotripyrrin-b. The LPS injection markedly increased bilirubin oxidative metabolites in urine of rats fed an ascorbic acid-free diet. This increase was suppressed by feeding an enough amount of ascorbic acid, a physiological antioxidant. The concentrations of biotripyrrin-a and -b in urine collected 6.5-10h after the LPS injection were lower in rats fed an ascorbic acid-supplemented diet than in rats fed an ascorbic acid-free diet. Moreover, feeding an ascorbic acid suppressed the elevation of hepatic mRNA level of heme oxygenase-1, the rate-limiting enzyme of bilirubin biosynthesis, in rats injected with LPS.These findings suggest that bilirubin is oxidized in rats treated with LPS and acts as a physiological antioxidant synergistically with ascorbic acid in vivo.
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Research Products
(11 results)
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[Publications] Yamaguchi, T., Hashizume, T., Tanaka, M., Nakayama, M., Sugimoto, A., Ikeda, S., Nakajima, H., and Horio, F.: "Bilirubin oxidation provoked by endotoxin treatment is suppressed by feeding ascorbic acid in a rat mutant unable to synthesize ascorbic acid." Eur.J.Biochem. 245. 233-240 (1997)
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[Publications] Sano, T., Shimomi, M., Wakabayashi, Y., Shinoda, Y., Goda, N., Yamaguchi, T., Nimura, Y., Ishimura, Y., and Suematsu, M.: "Endogeneous carbon monoxide supression stimulates bile acid-dependent biliary transport in perfused rat liver." Am.J.Physiol.G1268-G1275 (1997)
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