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1997 Fiscal Year Final Research Report Summary

Regulation and Analysis of Neurovascular Network and Circulatory Status by Site-Directed Superoxide Dismutase

Research Project

Project/Area Number 08457054
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionOSAKA CITY UNIVERSITY

Principal Investigator

INOUE Masayasu  Osaka City Univ. (Department of Biochemistry, ) Medical School Professor, 医学部, 教授 (80040278)

Co-Investigator(Kenkyū-buntansha) SATO Eisuke  Osaka City Univ. (Department of Biochemistry, ) Medical School Asistant Professo, 医学部, 助手 (60211942)
Project Period (FY) 1996 – 1997
KeywordsActive oxygen / superoxide / nitric oxide / oxygen stress / blood pressure / hypertension / brain / mannose-receptor
Research Abstract

Kinetic analysis using long acting and endothelially targeted superoxide dismutase (SOD) revealed that superoxide and related reactive oxygen species play important role in the pathogenesis of vasogenic tissue injury. Hence, selective dismutation of superoxide radicals is of critical importance in preventing and/or treating patients with circulatory disturbance. Targeting SOD to mannose-receptor-containing cells resulted inn rapid decrease in blood pressure, suggesting that the receprot-containing cells are responsible for the maintenance of blood pressure. Biochemical analysis using radiolabeled mannose-linked SOD revealed that more than 90% of SOD was localized in hepatic Kupffer cells and splenic macrophages. However, splenolectomy and 75% hepatectomy did not affect the depressor action of SOD,suggesting that other tissue (s) than liver and spleen might be responsible for the maintenance of the circulatory status. Kinetic analysis using various anesthetics, atropine and guanethidine suggested that the mannose receptor containing cells might localize in central nervous system. Specific antibody against mannose-linked SOD and mannose-linked xanthine oxidase might permit studies on the determination of the receptor-containing cells in the brain and on the mechanism by which circulatory status is regulated by cross-talk of reactive oxygen species.

  • Research Products

    (67 results)

All Other

All Publications (67 results)

  • [Publications] Minamiyama, Y.;Takemura, S.;Kawada, N.;Inoue, M.: "Role of nitric oxide in extracellular nucleotide-induced contractile status of assorted vessels including parts of portal vasculature" J.Hepatology. 28. 314-319 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishikawa, M.;Sato, EF.;Kuroki, T.;Utsumi, K.;Inoue, M.: "Role of glutathione in energy metabolism of tumor cells" Hepatology. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakanishi, K.;Nishi, Y.;Sato, EF.;Ishii, M.;Hamada, T, ;Inoue, M.: "Termal injury induces thymoyte apoptosis in the rat" J.Trauma. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakahara, H.;Sato, EF.;Ishihara, R.;Yabuki, M, ;Inoue, M.;Utsumi, K.: "Biochemical properties of human neutrophils" Free Radic.Res.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishikawa, M.;Takeda, K.;Sato, EF.;Kuroki.T.;Inoue, M.: "Nitric oxide regulates energy metabolism and bcl-2 expression in rat interstional epithelial cells" Am.J.Physiol.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morishita, S.;Sato, EF.;Takahashi, YP.;Manabe, M., Inoue, M.: "Hypoglycemia-induced apoptosis in rat thymocytes in vivo" Dyabetes Res.Clin.Prac.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagata, K.;Yu, H.;Sato, EF.;Kasahiba, M.;Tamura, T.;Inoue, M.: "Irreversible inhibition of respiration of Helicobacter pylori by nitric oxide" J.Biol.Chem.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kawada, N.;Seki, S.;Inoue, M.;Kuroki, T.: "Effect of antioxidants,resveratol,quercetin and N-acetyl cysteine on the function of hepatic stellate cells and Kupffer cells" Hepatology. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Dunlap, WC.;Yamamoto, Y.;Inoue, M.;Kashiba, M.;Yamaguchi, M.;Tomita, K.: "Uric acid pjoto-oxidation assay : in vitro comparison of sunscreening agents" Int.I.Cosmetic Sci.20. 1-8 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] I.Shiraishi.;E.F.Sato.;M.Nishikawa.;F.Sendo.;M.Inoue: "Mechanism of hypotensive shock induced by anti-neutrophil monocional antibody" Pathopysiology. 4. 197-204 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Yu.;E.F.Sato.;Arakawa.;K.Kobayashi, .;M.Inoue: "Protective action of lipopolysaccharide against stress-induced gastric mucosal injury" Digestion. 58. 311-318 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirai, A.;Minamiyam, Y.;Hamada, T.;Ishii, M.;Inoue, M.: "Dynamic aspects of glutathione metabolism in mice with hapten-induced allergic and irritant contact" J.Invest.Dermatol.109. 314-318 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minamiyam, Y.;Takemura, S.;Inoue, M.: "Effect of thiol status on nitric oxide metabolism in the circulation" Arch.Biochem.Biophys.341. 186-192 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yabuki, M.;Inai, Y.;Yoshioka, T.;Hamazaki, K.;Yasuda, T.;Inoue, M.;Utsumi, K.: "Oxygen-dependent fragmentation of cellular DNA by nitric oxide" Free Rad.Res.26. 245-255 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uno, H.;Arakawa, T.;Fukuda, T.;Yu, H.;Fujiwara, Y.;Higuchi, K.;Inoue, M.;Kobavashi.K.: "Nitric oxide stimulates prostaglandin synthesis in cultured rabbit gastric cells" Prostaglandins. 53. 153-162 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yu, H.;Sato, EF.;Nagata, K.;Nishikawa, M.;Kashiba, M.;Arakawa, T.;Kobavashi.K.;Inoue.M.: "Oxygen-dependent regulation of the respiration and growth of Escherichia coli by nitric oxide" FEBS Letters. 409. 161-165 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamasu, S.;Sato, EF.;Ogita, S.;Inoue, M.: "Role of glutathione metabolism and apoptosis in the regression of liver hemopoiesis" Free Radical Biol.& Med.23. 100-109 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kashiba, M.;Miyamoto, M.;Inoue.M.: "Effect of nitric oxide on the ligand binding activity of albumin" Arch.Biochem.Biophys.345. 237-242 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morishita, S.;Nishi, Y.;Sato, EF.;Yamaoka, K.;Manabe, M.;Inoue.M.: "Cold-stress induces thymocyte apoptosis in the rat" Pathophysiology. 4. 213-219 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hanada, H.;Kashiwagi, A.;Takehara, Y.;Kanno, T.;Yabuki, M.;Sasaki, J.;Inoue.M.;Utsumi.K.: "Do reactive oxygen species underlie the mechanism of apoptosis in the tadpole tail?" Free Radical Biol.& Med.23. 294-301 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishikawa, M.;Sato, EF.;Kuroki, T.;Inoue.M.: "Role glutathione in the energy metabolism of mitochondria" FEBS Letters. 415. 341-345 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kashiba, M.;Kitoh, K.;Yu, H.;Kasahara, E.;Nishikawa, M.;Matsuo, M.;Inoue.M.: "Ascorbic Acid and reducing agents regulates the fates and functions of S-nitrospthiols" J.Biochem.122. 1208-1214 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minamiyama, Y.;Takemura, S.;Imaoka, S.;Funae, Y.;Tanimoto, Y.;Inoue.M.: "Irreversible inhibition of cytochrome P450 by nitric oxide" J.Phaemacol.Exp.Ther.283. 1479-1485 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada, T.;Yukioka, H.;Asada, A.;Inoue.M.: "Effect of inhaled nitric oxide on platelet-activating factor induced pulmonary hypertension in dogs" Acta Anesthesiol.Scand.41. (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kashiba-Iwatsuki.M., Kitoh, K.Nishikawa, M., Sato, FE, Inoue, M: "Oxygen Homeostasis and its dynamics" Springer-Verlag,Tokyo(Ishimura,Y.,Suematsu.M.,Shimada,H), 7 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Utsumi, K., Takchara, Y., Inai.Y., Yabuki, M., Kanno, T., Nishikawa, M., Sato, FE., Inoue, M.: "Mitochondria,Oxidants,and Aging" Academic Press (L.Packer Cadenas)(in press), (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fujino, Y. ; Ozaki, K. ; Yamamasu, S. ; Ito, F. ; Matsuoka, I. ; Hayashi, E. ; Nakamura, H. ; Ogita, S. ; Sato, E. ; Inoue, M.: "DNA fragmentation of oocytes in aged mice" Human Reproduction. 11(7). 1480-3 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iha, S. ; Orita, K. ; Manno, T. ; Utsumi, T. ; Sato, E.F. ; Inoue, M. ; Utsumi, K.: "Oxygen-dependent inhibition of neutrophil respiratory burst by nitric oxide" Free-Radic-Res. 25(6). 489-98 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inai, Y. ; Takehara, Y. ; Yabuki, M. ; Sato, E.F. ; Akiyama, J. ; Yasuda, T. ; Inoue, M. ; Horton, A.A. ; Utsumi, K.: "Oxygen-dependent-regulation of Ehrlich ascites tumor cell respiration by nitric oxide" Cell Structure & Function. 21(2). 151-7 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inoue, M. ; Minamiyama, Y. ; Takemura, S.: "Dynamic aspects of nitric oxide metabolism in the circulation and tissues" Methods in Enzymology. 269. 474-9 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Inoue, M. ; Inoue, K.: "Role of free radicals in and around vascular endothelial cells in the mechanism of aging" Annals of the New York Academy of Sciences. 786. 224-32 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kashiba Iwatsuki, M. ; Yamaguchi, M. ; Inoue, M.: "Role of ascorbic acid in the metabolism of S-nitroso-glutathione" FEBS-Lett. 389(2). 149-52 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawada, N. ; Kuroki, T. ; Uoya, M. ; Inoue, M. ; Kobayashi, K.: "Smooth muscle alpha-actin expression in rat hepatic stellate cell is regulated by nitric oxide and cGMP production" Biochemical & Biophysical Research Communication. 229(1). 238-42 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawada, N. ; Kuroki, T. ; Kobayashi, K. ; Inoue, M. ; Kaneda, K.: "Inhibition of myofibroblastic transformation of cultured rat hepatic stellate cells by methylxanthines and dibutyryl cAMP" Digestive Diseases & Sciences. 41(5). 1022-9 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawada, N. ; Kuroki, T. ; Kobayashi, K. ; Inoue, M. ; Nakatani, K. ; Kaneda, K. ; Nagata, K.: "Expression of heat-shock protein 47 in mouse liver" Cell & Tissue Research. 284(2). 341-6 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minamiyama, Y. ; Takemura, S. ; Koyama, K. ; Yu, H. ; Miyamoto, M. ; Inoue, M.: "Dynamic aspects of glutathione and nitric oxide metabolism in endotoxemic rats" American Journal of Physiology. 271(4Pt1). G575-81 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minamiyama, Y. ; Takemura, S. ; Inoue, M.: "Albumin is an important vascular tonus regulator as a reservoir of nitric oxide" Biochemical & Biophysical Research Communication. 225(1). 112-5 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishikawa, M. ; Sato, E.F. ; Utsumi, K. ; Inoue, M.: "Oxygen-dependent regulation of energy metabolism in ascites tumor cells by nitric oxide" Cancer Research. 56(19). 4535-40 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nomura, T. ; Sasaki, J. ; Mori, H. ; Sato, E.F. ; Watanabe, S. ; Kanda, S. ; Matsuura, J. ; Watanabe, H. ; Inoue, M.: "Expression of manganese superoxide dismutase mRNA in reproductive organs during the ovulatory process and the estrous cycle of the rat" Histochemistry & Cell Biology. 105(1). 1-6 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Okada, S. ; Takehara, Y. ; Yabuki, M. ; Yashioka, T. ; Yasuda, T. ; Inoue, M. ; Utsumi, K.: "Nitric oxide, a physiological modulator of mitochondrial function" Physiological Chemistry & Physics & Medical NMR. 28(2). 69-82 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Radak, Z. ; Asano, K. ; Inoue, M. ; Kizaki, T. ; Oh-Ishi, S. ; Suzuki, K. ; Taniguchi, N. ; Ohno, H.: "Superoxide dismutase derivative prevents oxidative damage in liver and kidney of rats induced by exhausting exercise" European Journal of Applied Physiology & Occupatio Physiology. 72(3). 189-94 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, E.F. ; Utsumi, K. ; Inoue, M.: "Human oral neutrophils : isolation and characterization" Methods in Enzymology. 268. 503-9 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sato, E.F. ; Yamaoka, K. ; Yu, H. ; Morishita, S. ; Inoue, M.: "Low dose X-ray irradiation induces stress proteins but does not prevent gastric mucosal lesion" Physiological Chemistry & Physics & Medical NMR. 28(2). 103-9 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] I.Shiraishi. ; E.F.Sato. ; M.Nishikawa. ; F.Sedo. ; M.Inoue.: "Mechanism of hypotensive shock induced by anti-neutrophil monoclonal antibody" Pathopysiology. 4. 197-204 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Yu. ; E.F.Sato. ; T.Arakawa. ; K.Kobayashi, . ; M.Inoue.: "Protective action of lipopolysaccharide against stress-induced gastric mucosal injury" Digestion. 58. 311-318 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirai, A. ; Minamiyama, Y. ; Hamada, T. ; Ishii, M. ; Inoue, M.: "Dynamic aspects of glutathione metabolism in mice with hapten-induced allergic and irritant contact" J.Invest.Dermatol. 109. 314-318 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minamiyama, Y. ; Takemura, S. ; Inoue, M.: "Effect of thiol status on nitric oxide metabolism in the circulation" Arch.Biochem.Biophys.341. 186-192 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yabuki, M. ; Inai, Y. ; Yoshioka, T. ; Hamazaki, K. ; Yasuda, T. ; Inoue, M. ; Utsumi, K.: "Oxygen-dependent fragmentation of cellular DNA by nitric oxide" Free Rad.Res.26. 245-255 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uno, H. ; Arakawa, T. ; Fukuda, T. ; Yu, H. ; Fujiwara, Y. ; Higuchi, K. ; Inoue, M. ; Kobayashi, K.: "Nitric oxide stimulates prostaglandin synthesis in cultured rabbit gastric cells" Prostaglandins. 53. 153-162 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yu, H. ; Sato, EF. ; Nagata, K. ; Nishikawa, M. ; Kashiba, M. ; Arakawa, T. ; Kobayashi, K. ; Inoue, M.: "Oxygen-dependent regulation of the respirarion and growth of Escherichia coli by nitric oxide" FEBS Letters. 409. 161-165 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamasu, S. ; Sato, EF. ; Ogita, S. ; Inoue, M.: "Role of glutathione metabolism and apoptosis in the regression of liver hemopoiesis" Free Radical Biol. & Med.23. 100-109 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kashiba, M. ; Miyamoto, M. ; Inoue, M.: "Effect of nitric oxide on the ligand binding activity of albumin" Arch.Biochem.Biophys.345. 237-242 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morishita, S. ; Nishi, Y. ; Sato, EF. ; Yamaoka, K. ; Manabe, M. ; Inoue, M.: "Cold-stress induces thymocyte apoptosis in the rat" Pathophysiology. 4. 213-219 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hanada, H. ; Kashiwagi, A. ; Takehara, Y. ; Kanno, T. ; Yabuki, M. ; Sasaki, J. ; Inoue, M. ; Utsumi, K.: "Do reactive oxygen species underlie the mechanism of apoptosis in the tadpole tail?" Free Radical Biol.& Med.23. 294-301 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishikawa, M. ; Sato, EF. ; Kuroki, T. ; Inoue, M.: "Role glutathione in the energy metabolism of mitochondria" FEBS Letters. 415. 341-345 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kashiba, M. ; Kitoh, K. ; Yu, H. ; Kasahara, E. ; Nishikawa, M. ; Matsuo, M. ; inoue, M.: "Ascorbic Acid and reducing agents regulates the fates and functions of S-nitrospthiols" J.Biochem.122. 1208-1214 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minamiyama, Y. ; Yakemura, S. ; Imaoka, S. ; Funae, Y. ; Tanimoto, Y. ; Inoue, M.: "Irreversible inhibition of cytochrome P450 by nitric oxide" J.Phaemacol.Exp.Ther.283. 1479-1485 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, T. ; Yukioka, H. ; Asada, A. ; Inoue, M.: "Effect of inhaled nitric oxide on platelet-activating factor induced pulmonary hypertension in dogs" Acta Anesthesiol.Scand.41. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minamiyama, Y. ; Takemura, S. ; Kawada, N. ; Inoue, M.: "Role of nitric oxide in extracellular nucleotide-induced contractile status of assorted vessels including parts of portal vasculature" J.Hepatology. 28. 314-319 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishikawa, M. ; Sato, EF. ; Kuroki, T. ; Utsumi, K. ; Inoue, M.: "Role of glutathione in energy metabolism of tumor cells" Hepatology. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakanishi, K. ; Nishi, Y. ; Sato, EF. ; Ishii, M. ; Hamada, T, ; Inoue, M.: "Termal injury induces thymoyte apoptosis in the rat" J.Trauma. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakahara, H. ; Sato, EF. ; Ishihara, R. ; Yabuki, M. ; Inoue, M. ; Utsumi, K.: "Biochemical properties of human neutrophils" Free Radic.Res.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishikawa, M. ; Takeda, K. ; Sato, EF. ; Kuroki.T. ; Inoue, M.: "Nitric oxide regulates energy metabolism and bcl-2 expression in rat intestional epithelial cells" Am.J.Physiol.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morishita, S. ; Sato, EF. ; Takahashi, YP. ; Manabe, M. ; Inoue, M.: "Hypoglycemia-induced apoptosis in rat thymocytes in vivo" Dyabetes Res.Clin.Prac.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagata, K. ; Yu, H. ; Sato, EF. ; Kasahiba, M. ; Tamura, T. ; Inoue, M.: "Trreversible inhibition of respiration of Helicobacter phlori by nitric oxide" J.Biol.Chem.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kawada, N. ; Seki, S. ; Inoue, M. ; Kuroki, T.: "Effect of antioxidants, resveratol, quercetin and N-acetyl cysteine on the function of hepatic stellate cells and Kupffer cells" Hepatology. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Dunlap, WC. ; Yamamoto, Y. ; Inoue, M. ; Kashiba, M. ; Yamaguchi, M. ; Tomita, K.: "Uric acid photo-oxidation assay : in vitro comparison of sunscreening agents" Int.I.Cosmetic Sci.20. 1-8 (1998)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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