1996 Fiscal Year Annual Research Report

前立腺がんの発生・進展と癌関連遺伝子異常

Research Project

Project/Area Number	08457060
Research Category	Grant-in-Aid for Scientific Research (B)
Research Institution	Mie University
Principal Investigator	矢谷隆一三重大学, 医学部, 教授 (80024636)
Co-Investigator(Kenkyū-buntansha)	渡辺昌俊三重大学, 医学部, 助手 (90273383) 白石泰三三重大学, 医学部・附属病院, 助教授 (30162762)
Keywords	前立腺がん / 多段階発がん / がん関連遺伝子
Research Abstract	本講座に保管されている前立腺がん凍結標本及びホルマリン固定パラフィン包埋標本を用いて、潜在癌、各臨床病期の前立腺がんのがん関連遺伝子変異及び遺伝子不安定性について分子生物学的手法を用いて、臨床病理学的検討を加え、前立腺がんの進展について解析中である。23個のマイクロサテライトマーカーを用いて、遺伝子不安定性について解析した。潜在癌は浸潤型を中心に8%の頻度で、臨床がんは14%の頻度で確認された。潜在癌或いは臨床病期早期から遺伝子不安定性が認められた。がん関連遺伝子に関して、p53及びAPC遺伝子変異について、PCR-SSCP法にて解析した。p53遭伝子変異に関して、臨床病期Dを中心に、12%の頻度で認められた。また、その変異様式は、欧米の前立腺がんと異なり、transversionが55%、transitionは36%の頻度で確認され、前立腺がん発がん、進展に関して影響を与える因子が異なるのではないかと推察された。APC遺伝子に関して、同遺伝子の存在する5q21のLOHが進行がんに多いと報告され、其の関与が疑われていた。しかし、臨床病期Bがん一例(3%)に、またLOHは16%に認められただけで、其の関与は少ないものと考えられた。アンドロゲンレセプター遺伝子の異常に関して、全coding regionに関してPCR-SSCP法を用いて36症例について解析した。5症例(14%)の変異を認め、臨床病期Dがん2症例がミスセンス変異、臨床病期A,B,D各1症例はexon Aに存在するCAGリピート数の変化を認めた。アンドロゲン不応性の獲得にレセプター遺伝子変異は一部に関与するものの、大部分は違うメカニズムによるものと推察された。現在、アンドロゲンレセプター遺伝子のプロモタ-領域の変異、BRCA2遺伝子変異及びLOHについて解析中である。

Research Products

(16 results)

All Other

All Publications (16 results)

[Publications] Tetsuya Murata: "Primary Non-Hodgkinn Malignant lymphoma of the male breast." Jpn.J.Clin.Oncol.26. 243-247 (1996)
[Publications] Hiroshi Matsuura: "Interphase cytogenetics of prostate cancer:Fluorescence in situ hybridization(FISH)analysis of Japanese cases." British J.Cancer. 74. 1699-1704 (1996)
[Publications] Masatoshi Watanabe: "Microsatellite instability in latent prostate cancers." Int.J.Cancer. 69. 394-397 (1996)
[Publications] Takeshi Ueda: "Expression of the KAII protein in benign prostatic hyperplasia and prostate cancer." Am.J.Pathol.149・5. 1434-1440 (1996)
[Publications] Hiroyoshi Suzuki: "Three distinct regions of chromosome and 16q in human primary and metastatic prostatic cancers." Genes,Chromosomes & Cancer. 17. 225-233 (1996)
[Publications] Akira Komiya: "Allelic losses at loci on chromosome 10 are associated with metastasis and progression of human prostate cancer." Genes,Chromosomes & Cancer. 17. 245-253 (1996)
[Publications] Noboru Konishi: "Detection of RB,p16/CDKN2 and p15^<INK4B> gene alterations with immunohistochemical studies in human prostate carcinomas." Int.J.Oncol.8. 107-112 (1996)
[Publications] Pradeep Vaidya: "Combined analysis of expression of c-erbB-2,Ki-67 antigen,and tenascin provides a better prognostic indicator of carcinoma of the papilla of vater." Pancreas. 12・2. 196-201 (1996)
[Publications] Masako Ochiai: "DNA adduct formation,cell proliferation and aberrant crypt induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine(PhIP)in rat colon relecance to carcinogenesis." Carcinogenesis. 17・1. 95-98 (1996)
[Publications] Masatoshi Watanabe: "APC gene mutation in human prostate cancer." Jpn.J.Clin.Oncol.26・2. 77-81 (1996)
[Publications] Takemasa Ohki: "Changes in histologic grade and argyrophilic nucleolar organizer regions during progression of prostate cancer." Jpn.J.Clin.Oncol.26・2. 82-87 (1996)
[Publications] Pradeep Vaidya: "Overexpression of different members of the type 1 growth factor receptor family and their association with cell proliferation in periampullary carcinoma." J.Pathol.
[Publications] Hideo Takeda: "Androgen receptor content of prostate carcinoma cells estimated by immunohisto-chemistry is related to prognosis of patients with stage D2 prostate carcinoma." Cancer. 77・5. 934-940 (1996)
[Publications] Minoru Toyota: "Genetic alterations in Rat colon tumors induced by heterocyclic amines." Cancer. 77・8. 1592-1597 (1996)
[Publications] Hiroyoshi Suzuki: "Detection of human papillomavirus DNA and p53 gene mutations in human prostate cancer." Prostate. 28. 318-324 (1996)
[Publications] Masaya Tsujii: "Active migration of fibroblasts derived from xeroderma pigmentosum in 3-dimensional collagen gel." Photomed.Photobiol.18. 129-130 (1996)

1996 Fiscal Year Annual Research Report

前立腺がんの発生・進展と癌関連遺伝子異常

Principal Investigator

矢谷 隆一 三重大学, 医学部, 教授 (80024636)

Research Products

[Publications] Tetsuya Murata: "Primary Non-Hodgkinn Malignant lymphoma of the male breast." Jpn.J.Clin.Oncol.26. 243-247 (1996)

[Publications] Hiroshi Matsuura: "Interphase cytogenetics of prostate cancer:Fluorescence in situ hybridization(FISH)analysis of Japanese cases." British J.Cancer. 74. 1699-1704 (1996)

[Publications] Masatoshi Watanabe: "Microsatellite instability in latent prostate cancers." Int.J.Cancer. 69. 394-397 (1996)

[Publications] Takeshi Ueda: "Expression of the KAII protein in benign prostatic hyperplasia and prostate cancer." Am.J.Pathol.149・5. 1434-1440 (1996)

[Publications] Hiroyoshi Suzuki: "Three distinct regions of chromosome and 16q in human primary and metastatic prostatic cancers." Genes,Chromosomes & Cancer. 17. 225-233 (1996)

[Publications] Akira Komiya: "Allelic losses at loci on chromosome 10 are associated with metastasis and progression of human prostate cancer." Genes,Chromosomes & Cancer. 17. 245-253 (1996)

[Publications] Noboru Konishi: "Detection of RB,p16/CDKN2 and p15^<INK4B> gene alterations with immunohistochemical studies in human prostate carcinomas." Int.J.Oncol.8. 107-112 (1996)

[Publications] Pradeep Vaidya: "Combined analysis of expression of c-erbB-2,Ki-67 antigen,and tenascin provides a better prognostic indicator of carcinoma of the papilla of vater." Pancreas. 12・2. 196-201 (1996)

[Publications] Masako Ochiai: "DNA adduct formation,cell proliferation and aberrant crypt induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine(PhIP)in rat colon relecance to carcinogenesis." Carcinogenesis. 17・1. 95-98 (1996)

[Publications] Masatoshi Watanabe: "APC gene mutation in human prostate cancer." Jpn.J.Clin.Oncol.26・2. 77-81 (1996)

[Publications] Takemasa Ohki: "Changes in histologic grade and argyrophilic nucleolar organizer regions during progression of prostate cancer." Jpn.J.Clin.Oncol.26・2. 82-87 (1996)

[Publications] Pradeep Vaidya: "Overexpression of different members of the type 1 growth factor receptor family and their association with cell proliferation in periampullary carcinoma." J.Pathol.

[Publications] Hideo Takeda: "Androgen receptor content of prostate carcinoma cells estimated by immunohisto-chemistry is related to prognosis of patients with stage D2 prostate carcinoma." Cancer. 77・5. 934-940 (1996)

[Publications] Minoru Toyota: "Genetic alterations in Rat colon tumors induced by heterocyclic amines." Cancer. 77・8. 1592-1597 (1996)

[Publications] Hiroyoshi Suzuki: "Detection of human papillomavirus DNA and p53 gene mutations in human prostate cancer." Prostate. 28. 318-324 (1996)

[Publications] Masaya Tsujii: "Active migration of fibroblasts derived from xeroderma pigmentosum in 3-dimensional collagen gel." Photomed.Photobiol.18. 129-130 (1996)

矢谷隆一三重大学, 医学部, 教授 (80024636)