1998 Fiscal Year Final Research Report Summary
Study on Anti-Cerebral Mararia Substances
| Project/Area Number |
08457083
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Tokai University |
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Principal Investigator |
AIKAWA Masamichi Tokai Univ.Res.Inst.of Med.Sci, Professor, 総合医学研究所, 教授 (90271593)
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| Co-Investigator(Kenkyū-buntansha) |
NAGAKURA Kouichi Tokai Univ.Med.Sch., Lecturer, 医学部, 講師 (80142454)
TORII Motomi Ehime Univ.Med.Sch., Professor, 医学部, 教授 (20164072)
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| Project Period (FY) |
1996 – 1998
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| Keywords | cerebral malaria / Plasmodium falciparum / invasive protozoan / atomic force microscopy / surface charge / artesunate / deferoxamine / pentoxiflyine |
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| Research Abstract |
1) Parasites infected erythrocytes (iRBCs) were examined by atomic farce microscopy (ARM). ARM revealed knobs, surface protrusions of Plasmodium falciparum or Babesia bovis-RBCs, have a positive charge and that the rest were negatively charged..
These results suggested that the positive charge of the knob plays an important role on the adhesion process of these iRBCs to negatively charged epithelluim cells of capillaries and erythiocytes. An iRBC adheres to several RBCs, reselting in rosette formation. The rosette formation process was inhibited by N-acetyl neuramic acid, a constitute of sialic acid.
2) Suramin and heparin inhibited adhesion of P.faalciparizm-iRBCs to C32 cells. ARM observations of P.falcipa.rum-iIlBCs treated with these reagents suggest that the inhibition was ascribed to neutrarization of the positive charge of the knob by the poly-anionic reagents.
3) AFM revealed that invasive forms of protozoan parasites (P.falciparum, Toxoplasma gondii, Leishmania amazonesis, Trypanosoma cruzz) have positively charged contact sit.. to their hostcells.
4) Combination of artesunate and deferoxamine or pentoxifylline were used for the treatment of patients with severe malaria at Bangkok Hospital for Tropical Desieases in Thailand. No antagonism between artesunate and deferoxamine was found and co-administration of these reagents hastened recovery from hepatocellular dysfunction. Effects of combination of artrsunate and pentoxifyiline were not different from these of artesuanate.
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[Publications] Aikawa, M., Kanamura, K., Shiraishi, S., Matsumoto, Y., Arwati, H., Torii, M., Ito, Y., Takeuchi, T.and Tandler: "B.Membrane knobs of unfixed Plasmodium falciparum-infected erythrocytes : New findings as revealed by atomic force microscopy and surface potential spectoscopy." Experimtl.Parasitol.84. 334-343 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Aikawa, M., Kawazu, S., Kamio, T., Matsumoto, Y., Naya, T., Torii, M., Ito, Y., Tandler, B., Nakano, Y., Shiraishi, S.and Kanamura, K.: "Membrane knobs of unfixed Babesia bovis-infected erythrocytes : new findings as revealed by atomic force microsocpy and surface potential spectroscopy, microscope and the surface potential microscope." Parasitol.International. 44. 241-246 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ahmed, K., Nakagawa, T., Nakano, Y., Martinez, G., Ichinose, A., Can, H.Z., Akaki, M., Aikawa, M.: "Attachment of Moraxella catarrhalis occursto positively charged domain of phyaryngeal epithelial cells." Infection and Immunity. (submitted for publication).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Akaki, M., Nakagawa, T., Nakano, Y., Takeuchi, T., Asai, T., Kobayashi, S., Nagakura, K., Katakura, K., Kawai, S., Matsumoto, Y., Horii, T.and Aikawa, M.: "Invasive protozoan parasites have a positive charge on their contact sites to the post cells." Nature Midicine. (submitted for publication).
Description
「研究成果報告書概要(欧文)」より
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[Publications] Looareesywan, S., Wilaritana, P., Pootrakul, P., Asawamhasakda, W., Yuthavong, Y., Fujioka, H., Gordeuk, V.R., McLaren, C.E., Meshnik, S.R., Aikawa, M., Brittenham, G.M.: "Randomized, double-blind prospective study of deferoxamine and artesunate in the treatment of severe malaria." NIH/ICIDR report. (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Looareesuwan, S., Wilairatana, P., Vannaphan, S., Wanaratana, V., Webisch, C., Aikawa, M., Brittenham, G., Graninger, W.and Wernsdorfer, W.H.: "Pentoxifylline as an ancillary treatment for severe falciparum malaria in Thailand." Am.J.Trop.Med.Hyg.58. 348-353 (1998)
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「研究成果報告書概要(欧文)」より
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[Publications] Akaki, M., Nakagawa, T., Nakano, Y., Takeuchi, T., Asai, T., Kobayashi, S., Nagakura, K., Katakura, K., Kawai, S., Matsumoto, Y., Horii, T.and Aikawa, M.: "Invasive protozoan parasites have a positive charge on their contact sites to the post cells." 68th Ann.Meeting of Jpn.Parasitol., April. (1999)
Description
「研究成果報告書概要(欧文)」より
