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1998 Fiscal Year Final Research Report Summary

Acquisition of Borna disease virus virulence by in vivo evolution

Research Project

Project/Area Number 08457091
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionHokkaido University

Principal Investigator

IKUTA Kazuyoshi  Institute of Immunological Science, Hokkaido University, Professor, 免疫科学研究所, 教授 (60127181)

Co-Investigator(Kenkyū-buntansha) IKEDA Kazuhiko  Tokyo Institute of Psychiatry, Head of Department, 精神医学総合研究所, 副参事研究員 (30124663)
Project Period (FY) 1996 – 1998
KeywordsBorna disease virus / schizophrenia / Parkinson disease / autopsy brain / gerbil / hippocampus / cerebellum / substantia nigra
Research Abstract

Borna disease virus (BDV) is neurotropic envelope virus with nonsegmented, single-stranded and minus-stranded RNA as a genome. We have reported that BDV is distributed in Japanese animals such as horses, sheep, cattle and cats. Further, we have reported that BDV prevalence was significantly higher in patients with schizophrenia and chronic fatigue syndrome, compared with blood donors. In this study, we have worked on the possible identification of a virulent BDV in human brains and obtained results as follows :
1) We detected BDV RNA in hippocampus, pons and cerebellum in one among four autopsied brains derived from schizophrenia patients. In contrast, there are no positive cases in several brain regions examined which were derived from 2 autopsied healthy brains.
2) We isolated human BDV from BDV RNA-positive hippocampus and cerebellum by inoculation of these brain regions to newborn gerbils followed by co-culture of the gerbil brain with human oligodendroglioma-derived cell line. 3) Full-length nucleotide sequence of BDV derived from the brain of a patient with schizophrenia was determined and compared with that of horse brain-derived BDV.The homology of human BDV with horse BDV at nucleotide sequence level was about 98%. Especially, the phosphoprotein (p24) and glycoprotein (gp 56) had many ammo acid substitutions. 4) We have examined the possible detection of BDV RNA in substantia nigra from patients with Parkinson's disease. Three among four from A hospital and three among 5 from B hospital were positive for BDV RNA BDV RNA was also positive in newborn gerbils intracranially inoculated with these BDV RNA-positive substantia nigras. However, we could not detect BDV replication in human oligodendroglioma-derived cell line, although we examined under the same conditions as in BDV isolation from the brain of a patient with schizophrenia.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Kobayashi, T., et al.: "Nuclear targeting activity associated with the amino terminal region of the Borna disease virus nucleoprotein." Virology. 245. 53-64 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwahashi, K., et al.: "Positive and negative syndromes, and Borna disease virus(BDV) infection in schizophrenia." Neuropsychobiology. 37. 59-64 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shoya, Y., et al.: "Two proline-rich nuclear localization signals in the amino- and carboxyl-terminal regions of Borna disease virus phosphoprotein" J.Virol.72. 9755-9762 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kohno, T., et al.: "Fine structure and morphogenesis of Borna disease virus(BDV)." J.Virol.in press. (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 生田和良: "ボルナ病" 人と動物の共通感染症(丸山務、倉田毅、森田千春 編). 印刷中.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中村百合恵、他: "ボルナ病ウイルス" 医学のあゆみ(新興再興感染症). 185・5. 345-348 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高橋宏和他: "精神疾患との関連性がみられるボルナ病ウイルス" 臨床検査. 42・6. 694-695 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 庄谷 祐子、他: "ボルナ病ウイルス" 化学療法の領域. 32・1. 1-7 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 朝長 啓造他: "ヒトの神経を冒す新しいウイルス感染症-ボルナ病ウイルス感染症-" 遺伝. 53・2. 1-5 (1999)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 生田 和良: "新しいウイルス感染症-中枢神経系疾患と関連するボルナ病ウイルス-" LABEAM. in press.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kobayashi, T., et al.: "Nuclear targeting activity associated with the amino terminal region of the Borna disease virus nucleoprotein." Virology. 245. 53-64 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwahashi, K., et al.: "Positive and negative syndromes, and Borna disease virus (BDV) infection in schizophrenia." Neuropsychobiology. 37. 59-64 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shoya, Y., et al.: "Two proline-rich nuclear localization signals in the amino- and carboxyl-terminal regions of Borna disease virus phosphoprotein." J.Virol.72. 9755-9762 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kohno, T., et al.: "Fine structure and morphogenesis of Borna disease virus (BDV)." J.Virol.73. 1,760-766 (1999)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-12-08  

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