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1997 Fiscal Year Final Research Report Summary

Molecular Mechanism of Virus-Induced Cell damage and its regulation

Research Project

Project/Area Number 08457096
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionMie University

Principal Investigator

ITO Yasuhiko  Mie university Faculty of Medicine Professor, 医学部, 教授 (00022872)

Co-Investigator(Kenkyū-buntansha) NISHIO Machiko  Mie university Faculty of Medicine Assistant Professor, 医学部, 助手 (70156040)
KAWANO Mitsuo  Mie university Faculty of Medicine Lecture, 医学部, 講師 (00234097)
TSURUDOME Masato  Mie university Faculty of Medicine Associate Professor, 医学部, 助教授 (50159042)
Project Period (FY) 1996 – 1997
KeywordsCELL FUSION / PARAMYXOVIRUS / FRP-1 / HIV
Research Abstract

We analyzed the mechanisms of cell fusion, multinucleated giant cell formation and virus entry using paramyxoviruses and HIV.We found an interesting cell fusion induction system, in which fusion (F) protein of paramyxovirus alone can induce multinucleated giant cells. We also found that there were two kinds of F protein of SV 5, that is, one F protein of SV 5 has an ability for induction cell fusion without HN protein and other F protein can induce cell fusion by an assistance of HN protein. There are three amino acid differences between these F proteins, and we identified amino acid responsible for inducibility of cell fusion by F protein alone.
We previously found that virus-induced cell fusion is regulated by some host cell factors, Fusion Regulatory Factors (FRPs)-1 and -2. We isolated 8 monoclonal antibodies directed against FRP-1 molecules and clarified their abilities. Anti-FRP-1 antibodies can be classified into three groups, namely, cell fusion enhancing antibodies, cell fusion suppressing antibodies and antibody showing no activity. Anti-FRP-1 antibody, both cell fusion enhancing and suppressing antibodies, can stimulate intracellular signaling. However, we could not find the difference between these antidodies-induced signaling. We also isolate 19 monoclonal antibodies directed against murine FRP-1. Murine FRP-1 was found to be alloantigens and to be identical to Ly 10 alloantigens.

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Kousuke Okamoto: "An anti-Fusion Regulatory Protein-1(FRP-1)Monoclonal Antibody Suppresses Human Parainflienza Type2Virus-Induced Cell Fusion" Journal of General Virology. VOL.78. 83-89 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kousuke Okamoto: "Paramyxovirus-induced Syncytium Cell Formation is Suppressed by a Dominant Negative Fusion Regulatory Protein-1(FRP-1)/CD98 Mutated Construct an Important Role of FRP-1 in Virus-induced Cell Fusion" Journal of General Virology. VOL.78. 775-783 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeru Suga: "Human Immunodeficiency Virus Type-1 Envelope Glycoprotein Gp120 Induce Expression of Fusion Regulatory Protein(HRP-1)/CD98 onCD4+ T Cells:Apossible Regulatory Mechanism of HIV-Induced Syncytium Formation" Medical Microbiology and Immunology. VOL.185. 237-243 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Machiko Nishio: "Mapping of Epitopes Recognized by Monoclonal Antibodies Directed Against the Phosphoprotein of Human Parainfluenza Virus Type2 and the Location of Its Multimerization" Journal of General Virology. VOL.78. 1303-1308 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nobutada Tabata: "Involvement of Various Protein Kinases in Anti-FRP-1/CD98/4F2mAb-Induced Cell Fusion Mediated by HIV gp 160:Protain Tyrosine Kinase Activation Provides an Early an Obligatory Signalin FRP-1-Induced Cell Fusion" Medical Microbiology and Immunology. VOL.186. 115-123 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kousuke Okamoto: "Enhancement of Human Parainfluenza Virus-induced Cell Fusion by Pradimicin Alow Molecular Weightmannose-Binding Antibiotic" Journal of General Virology. VOL.186. 101-108 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morihiro Ito: "Role of Single Amino Acid at the Amino Teminus of the Simian Virus 5F2 Subumit Syncytium Formation" Journal of Virology. VOL.71. 9855-9858 (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigeomi Higuchi: "Induction of Human Osteoclast-like Cell by Treatment of Blood Monocytes with Anti-fution Regulatory Protein-1/CD98 Monoclinal Antibodies" Journal of Bone and Mineral Research. VOL.23. 44-49 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masato Turutomo: "Identification of Regions on the Fution Protein of Human Parainfluenza Type2 Virus which are Required for Haemagglutinin-Neuraminidase Proteins to Promote Cell Fution" Journal of General Virology. VOL.79. 279-289 (1998)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasuhiko Ito: "A Functional Antigenomic Promoter for the Paramyxovirus Simain Virus 5requires Proper Spacing between an Essential Internal Segment and the 3'Teminus" Journal of Virology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideki Tsumura: "Mouse albantigen,Ly10,is Identical to Murine Fusion Regulatory Protein-1(mFRP-1)/4F2/CD98:Aberrant Expression of mFRP-1/Ly10 Allotypes in Cell Derived form CDF1 Mice Due to the Gene Deletion" Cellular Immunology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Okamoto, M.Tsurudome, S.Ohgimoto, M.Kawano, M.Nishio, H.Komada, M.Ito, Y.Sakakura, and Y.Ito: "An Anti-Fusion Regulatory Protein-1 (FRP-1) Monoclonal Antibody Suppresses Human Parainfluenza Type2 Virus-Induced Cell Fusion" J.General virology. 78. 83-89 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Okamoto, S.Ohgimoto, M.Nishio, M.Tsurudome, M.Kawano, H.Komada, M.Ito, Y.Sakakura, and Y.Ito: "Paramyxovirus-induced syncytium cell formation is suppressed by a dominant negative fusion regulatory protein-1 (FRP-1)/CD98 mutated construct an important role of FRP-1 in virus-induced cell fusion" J.General virology. 78. 775-783 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Suga, M.Tsurudome, M.Ito, S.Ohgimoto, N.Tabata, M.Nishio, M.Kawano, H.Komada, M.Ito, M.Sakurai and Y.Ito: "Human Immunodeficiency Virus Type-1 Envelope Glycoprotein gp 120 Induce Expression of Fusion Regulatory Protein (FRP)-1/CD98 on CD4+T Cells : A Possible Regulatory Mechanism of HIV-Induced Syncytium Formation" Medical Microbiology and Immunology. 185. 237-243 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Nishio, M.Tsurudome, M.Ito, N.Watanabe, M.Kawano, H.Komada and Y.Ito: "Mapping of Epitopes Recognized by Monoclonal Antibodies Directed Against the Phosphoprotein of Human Parainfluenza Virus Type 2 and the Location of Its Multimerization" J.General virology. 78. 1303-1308 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Tabata, M.Ido, S.Suga, S.Ohgimoto, M.Tsurudome, M.Kawano, M.Nishio, N.Komada and Y.Ito: "Involvement of Various Protein Kinases in Anti-FRP-1/CD98/4F2 mAb- Induced Cell Fusion Mediated by HIV gp160 : Protein Tyrosine Kinase Activation Provides an Eady an Obligatory Signal in FRP-1-Induced Cell Fusion" Medical Microbiology and Immunology. 186. 115-123 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Okamoto, T.Oki, Y.Igarashi, M.Tsurudome, M.Nishio, M.Kawano, H.Komada, M.Ito, Y.Sakakura, and Y.Ito: "Enhancement of human parainfluenza virus-induced cell fusion by pradimicin, alow molecular weight mannose-binding antibiotic" Medical Microbiology and Immunology. 186. 101-108 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Ito, M.Nishio, M.Kawano, S.Kusagawa, H.Komada, Y.Ito and M.Tsurudome: "Role of a single amino acid at the amino terminus of the simian virus 5F2 subunit in syncytium formation" J.Virology. 71. 9855-9858 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Higuchi, N.Tabata, M.Tajima, M.Ito, M.Tsurudome, A.Sudo, A.Uchida and Y.Ito: "Induction of Human Osteoclast-like Cells by Treatment of Blood Monocytes with Anti-fusion Regulatory Protein-1/CD98 Monoclonal Antibodies" J.Bone and Mineral Research. 23. 44-49 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Tsurudome, M.Ito, M.Nishio, M.Kawano, S.Kusagawa, H.Komada, and Y.Ito: "Identification of regions on the fusion protein of human parainfluenza type2 virus which are required for haemagglutinin-neuraminidase proteins to promote cell fusion" J.General virology. 79. 279-289 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murphy, Y.Ito, and G.D.Parks: "A functional antigenomic promoter for the paramyxovirus simian virus 5 requires proper spacing between an essential intemal segment and the 3'teminus" J.Virology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Tsumura, S.Kimura, M.Kawano, M.Tsurudome, K.Shimura and Y.Ito: "Mouse alloantigen, Ly10, Is Identical to Murine Fusion Regulatory Protein-1 (mFRP-1)/4F2/CD98 : Aberrant Expression of mFRP-1/Ly10 Allotypes in Cell Derived from CDF1 Mice due to the Gene Deletion." Cellular Immunology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-16  

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