1998 Fiscal Year Final Research Report Summary
Analysis of flavivirus-cross-reactive immune responses which contribute to the pathogenesis of dengue hemorrhagic fever
| Project/Area Number |
08457100
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | National Institute of Infectious Diseases (1998)
Kinki University (1996-1997) |
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Principal Investigator |
KURANE Ichiro National Institute of Infectious Diseases・Department of Virology I, Director, ウイルス第一部, 部長 (90278656)
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| Co-Investigator(Kenkyū-buntansha) |
TAKASAKI Tomohiko National Institute of Infectious Diseases・Department of virology I, Senior Scien, ウイルス第一部, 主任研究官 (20221351)
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| Project Period (FY) |
1996 – 1998
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| Keywords | Dengue virus / Japanese encephalitis virus / T lymphocyte / Dengue hemorrhagic fever |
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| Research Abstract |
The aim of the present researh is to elucidate the role of flavivirus-cross-reactive immune responses in the pathogenesis of dengue hemorrhagic fever. We analyzed T lymphocytes which were cross-reactive for dengue and Japanese encephalitis viruses (JEV), using murine and human lymphocytes. T lymphocytes obtained from JEV-immune donors responded to JEV, but also responded to dengue viruses to lower levels. JEV-reactive CD4+ T cell clones established from the same donors did not respond to dengue viruses ; however, some T cell clones responded to West Nile virus (WNV). This may partly due to higher levels of amino acid homology between JEV and WNV.These results also suggest that T lymphocytes cross-reactive for JEV and dengue viruses exist only at the low frequency. These T cell clones did not possess unique T cell receptor motifs.
We also analyzed murine T cell responses to JEV, in order to understand T cell responses cross-reactive for dengue and JEV.We analyzed induction of JEV-specific CD8+ cytotoxic T lymphocytes (CTL) in Balb/c mice, and attempted to determine epitope (s) recognized by specific CTL.JEV-specific CD8+CTL were induced by infection with JEV.There is one epitope on the envelope protein recognized by these CTL.The epitope is located between amino acids 60-68 on the envelope protein, and the recognition of this epitope is restricted by H-2Kd. We are analyzing flavivirus-cross-reactivity of these CTL, and determining other epitopes recognized by the CD8+CTL.
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