1997 Fiscal Year Final Research Report Summary
Regulatory mechanism of antigen-stimulated lymphocyte migration in intestinal mucosa
| Project/Area Number |
08457174
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Keio University |
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Principal Investigator |
MIURA Soichiro Keio University, Dept.of Medicine, Associate Professor, 医学部・内科, 助教授 (50138012)
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| Co-Investigator(Kenkyū-buntansha) |
SHIGEMATSU Takeharu Keio University, Department of Medicine, 医学部・内科, 助手 (10245483)
SERIZAWA Hiroshi Kitasato Institute Hospital, Department of Medicine, 付属病院・内科, 医員(研究職) (60187870)
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| Project Period (FY) |
1996 – 1997
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| Keywords | Peyer's patch / Tlymphocyte migration / Postcapillary venule / Endotoxin / Concanavalin A / PG-PS / alpha4-integrin / intestinal inflammation |
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| Research Abstract |
Lymphocytes from gut associated lymphoid tissue (GALT) continuously recirculate throughout systemic circulation and play an important role for intestinal mucosal defense. In this study, we have established an observatory system of lymphocyte migration in rat Peyer's patches using intravital fluorescence microscopy and investigated the altered migration pattem of stimulated T cells as compared with naive T cells. Con A-stimulation of T cells showed the loss of selective adherent properties in Peyer's patches as shown in naive T cells. On the other hand, the adherence of T cells to hepatic sinusoidal endothelium was significantly increased after Con A activation. The enhanced rolling and adherence of lymphocytes was observed in Peyer's patches and submucosal venules of villi when rats were treated with endotoxin, and these changes were mediated largely by alpha4- integrins.Intestinal inflammation was also induced by an intramural injection of peptidoglycan-polysaccharide (PG-PS) from group A streptococci into rat ileal Peyer's patches. We also demonstrated that alpha4-integrin molecule plays a role in the early stages of PG-PS induced T lymphocyte migration to intestinal villus mucosa and Peyer's patches. By this study, we were able to demonstrate the chatnges in T lymphocyte-endothelial interaction after lymphocyte activation and obtained informations about their regulatory mechanisms.
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Research Products
(20 results)
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[Publications] Miura, S., Tsuzuki, Y., Kurose, I., Suematsu, M., Shigematsu, T., Kimura, H., Higuchi, H., Serizawa, H., Yagita, H., Okumura, K., Granger, D.N.and Ishii, H.: "Endotoxin stimulates lymphocyte-endothelial interactions in rat intestinal Peyer's patches and villus mucosa." Am.J.Physiol.271 (Gastrointest.Liver Physiol.34). G282-G292 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Serizawa, H., Miura, S., Imaeda, H., Tanaka, S., Kimura, H., Tsuzuki, Y., Han, J-Y., Toda, K., Hamada, Y., Tsuchiya, M., and Ishii, H.: "Reversal of altered intestinal mucosal immunity in rats fed elemental diet by supplementation of oleic acid." J.Gastroenterol.Hepatol.11. 811-818 (1996)
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「研究成果報告書概要(欧文)」より
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[Publications] Fukumura, D., Miura, S., Kurose, I., Higuchi, H., Suzuki, H., Ebinuma, H., Han, J-Y., Watanabe, N., Wakabayashi, G., Kitajima, M.and Ishii, H.: "IL-1 is an important mediator for micricirculatory changes in endotoxin-induced intestinal mucosal damage." Dig.Dis Sci.41. 2482-2492 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Miura, S., Serizawa, H., Tsuzuki, Y., Kurose, I., Suematsu, M., Higichi, H., Shigematsu, T., Hokari, R., Hirokawa, M., Kimura, H.and Ishii, H.: "Vasoactive intestinal peptide modulates T lymphocyte migration in Peyer's patches of rat small intestine." Am.J.Physiol.(Gastrointest.Liver Physiol.35). G92-G99 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tanaka, S., Miura, S., Kimura, H., Ohkubo, N., Tsuzuki, Y., Fukumura, D., Serizawa, H., Kurose, I., Mori, M.and Ishii, H.: "Amelioration of chronic inflammation by injestion of elemental diet in a rat model of granulomatous enteritis." Dig.Dis Sci.42. 408-419 (1997)
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「研究成果報告書概要(欧文)」より
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[Publications] Tsuzuki, Y., Miura, S., Kurose, I., Suematsu, M., Higuchi, H., Shigematsu, T., Kimura, H., Serizawa, H., Hokari, R., Akiba, Y., Yagita, H., Okumura, K., Tso, P., Granger, D.N.and Ishii, H.: "Enhanced lymphocye interaction in postcapillary venules of Peyer's patches during fot absorption in rats." Gastroenterology. 112. 813-825 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kimura, H., Miura, S., Shigematsu, T., Ohkubo, N., Tsuzuki, Y., Kurose, I., Higuchi, H., Akiba, Y., Hokari, R., Hirokawa, M., Serizawa, H.and Ishii, H.: "Increased nitric oxide production and inducible nitric oxide synthase activity in colonic mucosa of patients with active ulcerative colitis and Crohn's disease." Dig.Dis.Sci.42. 1047-1054 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Hirokawa, M., Miura, S., Shigematus, T., Yoshida, H., Hokari, R., Higuchi, H., Kurose, I., Kimura, H., Saito, H., Nakaki, T.and Ishii, H.: "Pressure stimulates proliferation and DNA synthesis in rat intestinal epithelial cells." Life Sci.61. 667-672 (1997)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Kimura, H., Hokari, R., Miura, S., Shigematsu, T., Hirokawa, M., Akiba, Y., Kurose, I., Hiuchi, H., Fujimori, H., Tsuzuki, Y., Serizawa H.and Ishii, H.: "Increased expression of an inducible isoform of nitric oxide synthase and the formation of peroxynitrite in colonic mucosa of patients with active" Gut. 42. 180-187 (1998)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Miura, S., Tsuzuki, Y., Hokari, R., Serizawa, H., Kurose, I., Hirokawa, M., Higuchi, H., Shigematsu, T., Kimura, H., Suematsu, M., Tsuchiya, M.and Ishii, H.: Organ Microcirculation : Bridging between Basic and Clinical Sciences., eds.by Tsuchiya, M., Katori, M., Suematsu, M."Molecular mechanism of lymphocyte trafficking in Peyer's patches.". Excerpta Medica (Tokyo), 29-36 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Miura, S., Hokari, R., Tsuzuki, Y., Hirokawa, M., Kurose, I., Kimura, H., Serizawa, H., Suematsu, M., Yagita, H., Granger, D.N., Tsuchiya, M.and Ishii, H.: Sixth World Congress for Microcirculation, eds.by Messmer, K., Kubler, W.M., "Role of nitric oxide in lymphocyte-endothelium interaction in Peyer's patches and villus mucosa of rat small intestine.". Monduzzi Editore (Bologna, Italy), 637-641 (1996)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Fujimori, H., Miura, S., Hokari, R., Hirokawa, M., Kimura, H., Takeuchi, T., Yoshida, H., Higuchi, H., Kurose, I., Suzuki, H., Tsuzuki, Y., Serizawa, H., Suematsu, M.and Ishii, H.: Microcirculation annual 1997, eds.by Tsuchiya, M., Asano, M., Sato, N."Migration of activated T lymphocytes to hepatic sinusoid.". Nihon Igakukan (Tokyo), 165-166 (1997)
Description
「研究成果報告書概要(欧文)」より
