1996 Fiscal Year Annual Research Report

高血圧ラットの高血圧発症、維持における脳内NOの役割-アンチセンスによる脳内NO合成酵素発現抑制を用いての検討-

Research Project

Project/Area Number	08457217
Research Category	Grant-in-Aid for Scientific Research (B)
Research Institution	Kurume University
Principal Investigator	今泉勉久留米大学, 医学部, 教授 (60148947)
Co-Investigator(Kenkyū-buntansha)	鈴木哲九州大学, 医学部, 医員松岡秀洋久留米大学, 医学部, 助手 (80248393) 岩見元照久留米大学, 医学部, 助手 (90203405)
Keywords	中枢 / NO / ラット / L-NMMA / テレメトリー
Research Abstract	中枢神経系は血圧調節に重要な役割を果たす。一酸化窒素(NO)合成酵素は血管のみならず、血圧調節に重要な役割を果たす弧束核(NTS)や延髄腹側外野(VLM)に存在するので、中枢性NOは血圧調節に何らかの役割を担っている可能性がある。事実、NTSニューロンはNOの基質であるL-アルジニンに反応し、その活動性を増す。又NO合成阻害剤のL-NMMAの脳糟内やVLMへの急性投与は交感神経活動亢進、血圧上昇を来す。しかしこれらの実験は急性で、しかも麻酔下動物での実験であるので、中枢性NOが本当に慢性的に血圧調節、高血圧の発症に関与するかは不明である。そこで本年度は無麻酔、無拘束の条件下で中枢性NOの阻害が血圧に変化を来すかを検討した。(方法)正常血圧ウィスターラットの脳糟内にカニューレを挿入した。血圧及び心拍数は腹部大動脈内に挿入したカテーテルにより又テレメトリーシステムを用い記録した。脳糟内にL-NMMA又はD-NMMAを2.2nmol/Kg/min 1週間投与した。また別のグレープに3倍量のL-NMMAを投与した。(結果)L-NMMA又はD-NMMAの投与は脳のNOの量(NOx)を有意に低下させたにも関わらず、血圧及び心拍数に影響しなかった。3倍量のL-NMMAにおいても同様の結果であった。これらの事は中枢性NOが血圧調節に関与する可能性は無いか、あっても非常に小さいと考えられる。

Research Products
(14 results)

All Other

All Publications (14 results)

[Publications] Hidehiro Matsuoka: "Asymmetric dimethylarginine,an endogenous nitric oxide synthase inhibitor,in experimental hypertension." Hypertention. 29. 242-247 (1997)
[Publications] Hidehiro Matsuoka: "Chronic L-Arginine Administraction Attenuates Cardiac Hypertrophy in Spontaneously Hypertensive Rats." Hypertention. 27. 14-18 (1996)
[Publications] Osamu Tamai: "Single LDL-apheresis improves endothelium-dependent vasodilatation in hypercholesterolemic humans." Circulation. 95. 76-82 (1997)
[Publications] Hideo Yasukawa: "Inhibition of intimal hyperplasia after balloon injury by antibodies to lCAM-1 and LFA-1." Circulation. (in press).
[Publications] Keisuke Kohno: "Renal depressor mechanism of exercise therapy in patients with essential hypertension." Am J Hypertens. (in press).
[Publications] Akihiko Nakashima: "Renal denervation prevents intraglomerular platelet aggregation and glomerular injury induced by chronic inhibition of nitric oxide synthesis." Nephron. 73. 34-40 (1996)
[Publications] Tatsuya Hashino: "Aminophylline resuces cardiac ishemic pain during percutaneous transluminal angioplasty." J Am Coll Cardiol. 28. 1725-1731 (1996)
[Publications] Kazuya Ichiki: "Long-term smoking impairs platelet-derived nitric oxide release." Circulation. 94. 3109-3114 (1996)
[Publications] Takeshi Koyanagi: "DNA typing of HLA class II genes in Japanese patients with rheumatic heart disease." J Mol Cell Cardil. 28. 1349-1353 (1996)
[Publications] Makoto Tsuruta: "Hyperinsulinaemia as a predictor of hypertension : An 11-year follow-up study in Japan." J Hypertension. 14. 483-488 (1996)
[Publications] Toshihiko Kubo: "Transfer function analysis of central arc of aortic baroreceptor reflex in rabbits." Am J Physiol. 40. 1054-1062 (1996)
[Publications] Kazunori Kuwano: "Xanthine oxidase mediates cycle flow vareations in a canine model of coronary arterial thrombosis." Am J Physiol. 270. 1993-1999 (1996)
[Publications] 今泉勉: "医療の最前線" 的場恒孝.野中共平, 142 (1996)
[Publications] 松岡秀洋: "医学のあゆみ-NOのすべて" 平田結喜緒, 286 (1996)

1996 Fiscal Year Annual Research Report

高血圧ラットの高血圧発症、維持における脳内NOの役割-アンチセンスによる脳内NO合成酵素発現抑制を用いての検討-

Principal Investigator

今泉 勉 久留米大学, 医学部, 教授 (60148947)

Research Products

[Publications] Hidehiro Matsuoka: "Asymmetric dimethylarginine,an endogenous nitric oxide synthase inhibitor,in experimental hypertension." Hypertention. 29. 242-247 (1997)

[Publications] Hidehiro Matsuoka: "Chronic L-Arginine Administraction Attenuates Cardiac Hypertrophy in Spontaneously Hypertensive Rats." Hypertention. 27. 14-18 (1996)

[Publications] Osamu Tamai: "Single LDL-apheresis improves endothelium-dependent vasodilatation in hypercholesterolemic humans." Circulation. 95. 76-82 (1997)

[Publications] Hideo Yasukawa: "Inhibition of intimal hyperplasia after balloon injury by antibodies to lCAM-1 and LFA-1." Circulation. (in press).

[Publications] Keisuke Kohno: "Renal depressor mechanism of exercise therapy in patients with essential hypertension." Am J Hypertens. (in press).

[Publications] Akihiko Nakashima: "Renal denervation prevents intraglomerular platelet aggregation and glomerular injury induced by chronic inhibition of nitric oxide synthesis." Nephron. 73. 34-40 (1996)

[Publications] Tatsuya Hashino: "Aminophylline resuces cardiac ishemic pain during percutaneous transluminal angioplasty." J Am Coll Cardiol. 28. 1725-1731 (1996)

[Publications] Kazuya Ichiki: "Long-term smoking impairs platelet-derived nitric oxide release." Circulation. 94. 3109-3114 (1996)

[Publications] Takeshi Koyanagi: "DNA typing of HLA class II genes in Japanese patients with rheumatic heart disease." J Mol Cell Cardil. 28. 1349-1353 (1996)

[Publications] Makoto Tsuruta: "Hyperinsulinaemia as a predictor of hypertension : An 11-year follow-up study in Japan." J Hypertension. 14. 483-488 (1996)

[Publications] Toshihiko Kubo: "Transfer function analysis of central arc of aortic baroreceptor reflex in rabbits." Am J Physiol. 40. 1054-1062 (1996)

[Publications] Kazunori Kuwano: "Xanthine oxidase mediates cycle flow vareations in a canine model of coronary arterial thrombosis." Am J Physiol. 270. 1993-1999 (1996)

[Publications] 今泉 勉: "医療の最前線" 的場恒孝.野中共平, 142 (1996)

[Publications] 松岡秀洋: "医学のあゆみ-NOのすべて" 平田結喜緒, 286 (1996)

今泉勉久留米大学, 医学部, 教授 (60148947)

[Publications] 今泉勉: "医療の最前線" 的場恒孝.野中共平, 142 (1996)