1997 Fiscal Year Final Research Report Summary
Regulation of protein and platelet adsorption on artificial materials with electrical field
| Project/Area Number |
08457296
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | University of Tokyo |
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Principal Investigator |
CHINZEI Tsuneo University of Tokyo, Graduate School of Medicine, Instructor, 大学院・医学系研究科, 助手 (20197643)
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| Co-Investigator(Kenkyū-buntansha) |
MABUCHI Kunihiko University of Tokyo, Center for Collaborative Research, Professor, 産学共同研究センター, 教授 (50192349)
IMACHI Kou University of Tokyo, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (10010076)
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| Project Period (FY) |
1996 – 1997
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| Keywords | protein adsorption / evanescent light / electric field |
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| Research Abstract |
In this study, the effect of electric field to regulate protein and platelet adsorption on artificial material was investigated. Protein and platelet adsorption was measured with evanescent light quantitatively.
1. Protein and platelet adsorption
Protein and platelet adsorption on polyurethane was measured. Comparative assay was performed among albumin (Alb), gammer-globulin (Glb), mixture of them and whole blood (Wbl). In Alb-PBS,multi-layr adsorption was observed in situ, mono-layr remained after washout. In Glb-PBS,mono-layr adsorption was observed and the thickness after washout was almost same as before. In Alb-Glb-PBS,multi-layr in situ, no change after washout. In Wbl, thick adsorption was observed and was decreased partialy after washout.
2. Adsorption regulation with electric field
Electrical potential was applied on polyurethane surface which was spin-coated on gold electrode at thickness of 2 micrometer. Protein adsorption was observed with evanescent light as above. Initially, Electrical potential suppressed albumin adsorption. After adsorption, however, Electrical potential could not remove adsorbed albumin, totally.
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